Paternal age effect of YY aneuploidy in human sperm, as assessed by fluorescence in situ hybridization

1997 ◽  
Vol 78 (2) ◽  
pp. 116-119 ◽  
Author(s):  
B. Kinakin ◽  
A. Rademaker ◽  
R. Martin
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Human Sperm ◽  
Age Effect ◽  
Paternal Age ◽  
Paternal Age Effect

Three-probe fluorescence in situ hybridization to assess chromosome X, Y, and 8 aneuploidy in sperm of 14 men from two healthy groups: evidence for a paternal age effect on sperm aneuploidy

1995 ◽  
Vol 7 (4) ◽  
pp. 799 ◽  
Author(s):  
WA Robbins ◽  
JE Baulch ◽  
D Moore ◽  
HU Weier ◽  
D Blakey ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Preliminary Finding ◽  
Human Sperm ◽  
Age Effect ◽  
Chromosome 8 ◽  
Paternal Age ◽  
Sperm Aneuploidy ◽  
Paternal Age Effect

The method of simultaneous three-chromosome fluorescence in situ hybridization (FISH) was developed using repetitive DNA sequence probes for chromosomes 8, X and Y and applied to semen of 14 men from two healthy groups who differed in their average ages (46.8 +/- 3.1 years, n = 4 v. 28.9 +/- 5.0 years, n = 10). The frequencies of disomic sperm determined by FISH compared well with frequencies obtained using the hamster-egg technique for human-sperm cytogenetics and with the frequencies of disomic and diploid sperm reported in previous FISH studies in this laboratory. The two groups of men did not differ in their baseline frequencies of sperm disomic for chromosome 8 (approximately 6.5 per 10(4) sperm), sperm with XY8 aneuploidy (approximately 9.5 per 10(4) sperm), or sperm with autodiploidy XX88 or YY88 (approximately 2 per 10(4) sperm). However, the older group had statistically higher frequencies of sperm carrying sex chromosomal disomy than the younger group (5.1 v. 2.2 per 10(4) sperm for XX8; 5.9 v. 2.0 per 10(4) sperm for YY8; P < 0.005). A recent report from this laboratory of sex-chromosomal aneuploidy in sperm of aged mice provides inter-species corroborating evidence for this preliminary finding of a paternal age effect on sperm aneuploidy in human males.

Non-disjunction in human sperm: results of fluorescence in situ hybridization studies using two and three probes

1993 ◽  
Vol 2 (11) ◽  
pp. 1929-1936 ◽  
Author(s):  
Briana J. Williams ◽  
Cynthia A. Ballenger ◽  
Henry E. Malter ◽  
Felicia Bishop ◽  
Michael Tucker ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Human Sperm

Estimation of aneuploidy levels for 8, 15, 18, X and Y chromosomes in 97 human sperm samples using fluorescence in situ hybridization

1997 ◽  
Vol 67 (6) ◽  
pp. 1134-1139 ◽  
Author(s):  
Frédéric Morel ◽  
Sylvie Mercier ◽  
Christophe Roux ◽  
Marie Claire Clavequin ◽  
Jean-Luc Bresson
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Human Sperm ◽  
Y Chromosomes

Fluorescence in Situ Hybridization Studies on the Sex Chromosome Constitution of Human Sperm

1997 ◽  
Vol 39 (3) ◽  
pp. 217-222 ◽  
Author(s):  
T. Dineen ◽  
A. Nolan ◽  
J. Harrington ◽  
A. Greer ◽  
R. Kennedy ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Sex Chromosome ◽  
Human Sperm ◽  
Chromosome Constitution

Evaluating sex chromosome content of sorted human sperm samples with use of dual-color fluorescence in situ hybridization

1997 ◽  
Vol 176 (6) ◽  
pp. 1172-1180 ◽  
Author(s):  
W.Edward Richards ◽  
Sheila M. Dobin ◽  
Vicki Malone ◽  
Alfred B. Knight ◽  
Thomas J. Kuehl
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Sex Chromosome ◽  
Human Sperm ◽  
Dual Color
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