FISH localization of human cytoplasmic actin genes ACTB to 7p22 and ACTG1 to 17q25 and characterization of related pseudogenes

1996 ◽  
Vol 74 (3) ◽  
pp. 221-224 ◽  
Author(s):  
H. Ueyama ◽  
J. Inazawa ◽  
H. Nishino ◽  
I. Ohkubo ◽  
T. Miwa
Keyword(s):  
Actin Genes ◽  
Cytoplasmic Actin

Characterization of Human Retinal Fascin Gene (FSCN2) at 17q25: Close Physical Linkage of Fascin and Cytoplasmic Actin Genes

Genomics ◽  
2000 ◽  
Vol 65 (2) ◽  
pp. 146-156 ◽  
Author(s):  
Benjamin E. Tubb ◽  
Soraya Bardien-Kruger ◽  
Catherine D. Kashork ◽  
Lisa G. Shaffer ◽  
Louis S. Ramagli ◽  
...  
Keyword(s):  
Actin Genes ◽  
Cytoplasmic Actin ◽  
Physical Linkage

scholarly journals Isolation and characterization of rat skeletal muscle and cytoplasmic actin genes.

1982 ◽  
Vol 79 (9) ◽  
pp. 2763-2767 ◽  
Author(s):  
U. Nudel ◽  
D. Katcoff ◽  
R. Zakut ◽  
M. Shani ◽  
Y. Carmon ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Rat Skeletal Muscle ◽  
Actin Genes ◽  
Cytoplasmic Actin ◽  
Isolation And Characterization

Molecular cloning and characterization of actin genes from Echinococcus granulosus

1993 ◽  
Vol 60 (2) ◽  
pp. 209-219 ◽  
Author(s):  
Cláudia M.D. da Silva ◽  
Henrique B. Ferreira ◽  
Marina Picón ◽  
Nicole Gorfinkiel ◽  
Ricardo Ehrlich ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Echinococcus Granulosus ◽  
Actin Genes

scholarly journals Isolation and characterization of human actin genes.

1981 ◽  
Vol 78 (8) ◽  
pp. 4674-4678 ◽  
Author(s):  
J. N. Engel ◽  
P. W. Gunning ◽  
L. Kedes
Keyword(s):  
Actin Genes ◽  
Isolation And Characterization

Molecular cloning and characterization of mutant and wild-type human beta-actin genes

1984 ◽  
Vol 4 (10) ◽  
pp. 1961-1969
Author(s):  
J Leavitt ◽  
P Gunning ◽  
P Porreca ◽  
S Y Ng ◽  
C S Lin ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Human Fibroblasts ◽  
Actin Gene ◽  
Actin Genes ◽  
Beta Actin ◽  
Gene Libraries ◽  
Actin Mrna ◽  
Dna Fragment ◽  
Specific Sequences

There are more than 20 beta-actin-specific sequences in the human genome, many of which are pseudogenes. To facilitate the isolation of potentially functional beta-actin genes, we used the new method of B. Seed (Nucleic Acids Res. 11:2427-2446, 1983) for selecting genomic clones by homologous recombination. A derivative of the pi VX miniplasmid, pi AN7 beta 1, was constructed by insertion of the 600-base-pair 3' untranslated region of the beta-actin mRNA expressed in human fibroblasts. Five clones containing beta-actin sequences were selected from an amplified human fetal gene library by homologous recombination between library phage and the miniplasmid. One of these clones contained a complete beta-actin gene with a coding sequence identical to that determined for the mRNA of human fibroblasts. A DNA fragment consisting of mostly intervening sequences from this gene was then used to identify 13 independent recombinant copies of the analogous gene from two specially constructed gene libraries, each containing one of the two types of mutant beta-actin genes found in a line of neoplastic human fibroblasts. The amino acid and nucleotide sequences encoded by the unmutated gene predict that a guanine-to-adenine transition is responsible for the glycine-to-aspartic acid mutation at codon 244 and would also result in the loss of a HaeIII site. Detection of this HaeIII polymorphism among the fibroblast-derived clones verified the identity of the beta-actin gene expressed in human fibroblasts.

scholarly journals Characterization of actin genes in Bonamia ostreae and their application to phylogeny of the Haplosporidia

Parasitology ◽  
2007 ◽  
Vol 134 (14) ◽  
pp. 1941-1948 ◽  
Author(s):  
I. LÓPEZ-FLORES ◽  
V. N. SUÁREZ-SANTIAGO ◽  
D. LONGET ◽  
D. SAULNIER ◽  
B. CHOLLET ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Protozoan Parasite ◽  
Ostrea Edulis ◽  
Coding Region ◽  
Actin Genes ◽  
Bonamia Ostreae ◽  
Flat Oyster ◽  
Primer Sets ◽  
Systemic Infections

SUMMARYBonamia ostreae is a protozoan parasite that infects the European flat oyster Ostrea edulis, causing systemic infections and resulting in massive mortalities in populations of this valuable bivalve species. In this work, we have characterized B. ostreae actin genes and used their sequences for a phylogenetic analysis. Design of different primer sets was necessary to amplify the central coding region of actin genes of B. ostreae. Characterization of the sequences and their amplification in different samples demonstrated the presence of 2 intragenomic actin genes in B. ostreae, without any intron. The phylogenetic analysis placed B. ostreae in a clade with Minchinia tapetis, Minchinia teredinis and Haplosporidium costale as its closest relatives, and demonstrated that the paralogous actin genes found in Bonamia resulted from a duplication of the original actin gene after the Bonamia origin.

scholarly journals Molecular cloning and characterization of mutant and wild-type human beta-actin genes.

1984 ◽  
Vol 4 (10) ◽  
pp. 1961-1969 ◽  
Author(s):  
J Leavitt ◽  
P Gunning ◽  
P Porreca ◽  
S Y Ng ◽  
C S Lin ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Human Fibroblasts ◽  
Actin Gene ◽  
Actin Genes ◽  
Beta Actin ◽  
Gene Libraries ◽  
Actin Mrna ◽  
Dna Fragment ◽  
Specific Sequences

There are more than 20 beta-actin-specific sequences in the human genome, many of which are pseudogenes. To facilitate the isolation of potentially functional beta-actin genes, we used the new method of B. Seed (Nucleic Acids Res. 11:2427-2446, 1983) for selecting genomic clones by homologous recombination. A derivative of the pi VX miniplasmid, pi AN7 beta 1, was constructed by insertion of the 600-base-pair 3' untranslated region of the beta-actin mRNA expressed in human fibroblasts. Five clones containing beta-actin sequences were selected from an amplified human fetal gene library by homologous recombination between library phage and the miniplasmid. One of these clones contained a complete beta-actin gene with a coding sequence identical to that determined for the mRNA of human fibroblasts. A DNA fragment consisting of mostly intervening sequences from this gene was then used to identify 13 independent recombinant copies of the analogous gene from two specially constructed gene libraries, each containing one of the two types of mutant beta-actin genes found in a line of neoplastic human fibroblasts. The amino acid and nucleotide sequences encoded by the unmutated gene predict that a guanine-to-adenine transition is responsible for the glycine-to-aspartic acid mutation at codon 244 and would also result in the loss of a HaeIII site. Detection of this HaeIII polymorphism among the fibroblast-derived clones verified the identity of the beta-actin gene expressed in human fibroblasts.

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