Identification of complex chromosome rearrangements in the gibbon by fluorescent in situ hybridization (FISH) of a human chromosome 2q specific microlibrary, yeast artificial chromosomes, and reciprocal chromosome painting

1996 ◽  
Vol 74 (1-2) ◽  
pp. 80-85 ◽  
Author(s):  
N. Arnold ◽  
R. Stanyon ◽  
A. Jauch ◽  
P. O’Brien ◽  
J. Wienberg
Keyword(s):  
In Situ Hybridization ◽  
Human Chromosome ◽  
Chromosome Painting ◽  
Fluorescent In Situ Hybridization ◽  
Chromosome Rearrangements ◽  
Artificial Chromosomes ◽  
Reciprocal Chromosome Painting ◽  
Yeast Artificial Chromosomes ◽  
Complex Chromosome

scholarly journals Mapping chromosome rearrangement breakpoints to the physical map of Caenorhabditis elegans by fluorescent in situ hybridization.

Genetics ◽  
1993 ◽  
Vol 134 (1) ◽  
pp. 211-219
Author(s):  
D G Albertson
Keyword(s):  
In Situ Hybridization ◽  
Caenorhabditis Elegans ◽  
Fluorescent In Situ Hybridization ◽  
Physical Map ◽  
Chromosome Rearrangements ◽  
Homologous Region ◽  
Genetic Constitution ◽  
Artificial Chromosomes ◽  
Degenerate Oligonucleotide

Abstract A scheme for rapidly mapping chromosome rearrangements relative to the physical map of Caenorhabditis elegans is described that is based on hybridization patterns of cloned DNA on meiotic nuclei, as visualized by fluorescent in situ hybridization. From the nearly complete physical map, DNA clones, in yeast artificial chromosomes (YACs), spanning the rearrangement breakpoint were selected. The purified YAC DNAs were first amplified by degenerate oligonucleotide-primed polymerase chain reaction, then reamplified to incorporate fluorescein dUTP or rhodamine dUTP. The site of hybridization was visualized directly (without the use of antibodies) on meiotic bivalents. This allows chromosome rearrangements to be mapped readily if the duplicated, deficient or translocated regions do not pair with a normal homologous region, because the site or sites of hybridization of the probe on meiotic prophase nuclei will be spatially distinct. The pattern, or number, of hybridization signals from probes from within, or adjacent to, the rearranged region of the genome can be predicted from the genetic constitution of the strain. Characterization of the physical extent of the genetically mapped rearrangements places genetic landmarks on the physical map, and so provides linkage between the two types of map.

scholarly journals Generation of a whole chromosome painting probe from monochromosomal hybrid cells by the alu-polymerase chain reaction

2007 ◽  
Vol 59 (2) ◽  
pp. 89-95 ◽  
Author(s):  
Danijela Drakulic ◽  
Gordana Nikcevic ◽  
Vesna Djordjevic ◽  
Milena Stevanovic
Keyword(s):  
Chromosome Painting ◽  
Fluorescent In Situ Hybridization ◽  
Chromosomal Rearrangements ◽  
Painting Probe ◽  
Complex Chromosomal Rearrangements ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Complex Chromosome ◽  
Whole Chromosome Painting

Fluorescent in situ hybridization (FISH) has become a widespread technique applicable in basic science and diagnostics. Chromosome painting represents a special application of FISH that has found increasing use in identification of complex chromosome rearrangements. Here we present a version of the Alu-PCR method modified to generate a whole chromosome painting probe (WCP) for human chromosome 19 using monochromosomal cell hybrids. In setting up conditions for this method, we established a cheap and fast approach to generation of WCPs for other human chromosomes that could be particularly useful for unambiguous identification of complex chromosomal rearrangements associated with cancer. .

Sequence-independent amplification and labeling of yeast artificial chromosomes for fluorescence in situ hybridization

1994 ◽  
Vol 65 (1-2) ◽  
pp. 108-110 ◽  
Author(s):  
S.K. Bohlander ◽  
R. Espinosa III ◽  
A.A. Fernald ◽  
J.D. Rowley ◽  
M.M. Le Beau ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Artificial Chromosomes ◽  
Yeast Artificial Chromosomes

scholarly journals Defining the Deletion Size in Williams-Beuren Syndrome by Fluorescent In Situ Hybridization with Bacterial Artificial Chromosomes

10.5772/33238 ◽  
2011 ◽  
Author(s):  
Audrey Basinko ◽  
Nathalie Douet-Guilbert ◽  
Severine Audebert-Bellanger ◽  
Philippe Parent ◽  
Clemence Chabay-Vichot ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Bacterial Artificial Chromosomes ◽  
Artificial Chromosomes ◽  
Deletion Size
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