Cloning, expression pattern and mapping to 12p13.2→p13.1 of CLAPS3, a gene encoding a novel clathrin-adaptor small chain

1996 ◽  
Vol 73 (3) ◽  
pp. 214-217 ◽  
Author(s):  
T.K. Watanabe ◽  
F. Shimizu ◽  
M. Nagata ◽  
A. Takaichi ◽  
T. Fujiwara ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Gene Encoding ◽  
Clathrin Adaptor ◽  
Small Chain

Cloning, expression pattern and mapping to Xq of NAP1 L3, a gene encoding a peptide homologous to human and yeast nucleosome assembly proteins

1996 ◽  
Vol 74 (4) ◽  
pp. 281-285 ◽  
Author(s):  
T.K. Watanabe ◽  
T. Fujiwara ◽  
Y. Nakamura ◽  
Y. Hirai ◽  
H. Maekawa ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Nucleosome Assembly ◽  
Gene Encoding ◽  
Assembly Proteins

scholarly journals Cloning and Expression Pattern of a Gene Encoding an α-Xylosidase Active against Xyloglucan Oligosaccharides from Arabidopsis

2001 ◽  
Vol 126 (2) ◽  
pp. 910-920 ◽  
Author(s):  
Javier Sampedro ◽  
Carmen Sieiro ◽  
Gloria Revilla ◽  
Tomás González-Villa ◽  
Ignacio Zarra
Keyword(s):  
Expression Pattern ◽  
Cloning And Expression ◽  
Gene Encoding

scholarly journals NECAPs are negative regulators of the AP2 clathrin adaptor complex

2017 ◽  
Author(s):  
Gwendolyn M Beacham ◽  
Edward A Partlow ◽  
Jeffrey J Lange ◽  
Gunther Hollopeter
Keyword(s):  
Eukaryotic Cells ◽  
Active Conformation ◽  
Negative Regulators ◽  
Gene Encoding ◽  
Transmembrane Receptors ◽  
Inactive Conformation ◽  
Clathrin Adaptor ◽  
Biochemical Analyses

ABSTRACTEukaryotic cells internalize transmembrane receptors via clathrin-mediated endocytosis, but it remains unclear how the machinery underpinning this process is regulated. We recently discovered that membrane-associated muniscin proteins such as FCHo and SGIP initiate endocytosis by converting the AP2 clathrin adaptor complex to an open, active conformation that is then phosphorylated (Hollopeter et al., 2014). Here we report that loss of ncap-1, the sole C. elegans gene encoding an adaptiN Ear-binding Coat-Associated Protein (NECAP), bypasses the requirement for FCHO-1. Biochemical analyses reveal AP2 accumulates in an open, phosphorylated state in ncap-1 mutant worms, suggesting NECAPs promote the closed, inactive conformation of AP2. Consistent with this model, NECAPs preferentially bind open and phosphorylated forms of AP2 in vitro and localize with constitutively open AP2 mutants in vivo. NECAPs do not associate with phosphorylation-defective AP2 mutants, implying that phosphorylation precedes NECAP recruitment. We propose NECAPs function late in endocytosis to inactivate AP2.

Molecular Evolution of C4 Phosphoenolpyruvate Carboxylase in the Genus Flaveria

1997 ◽  
Vol 24 (4) ◽  
pp. 429 ◽  
Author(s):  
Peter Westhoff ◽  
Per Svensson ◽  
Karin Ernst ◽  
Oliver Bläsing ◽  
Janet Burscheidt ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Phosphoenolpyruvate Carboxylase ◽  
Orthologous Gene ◽  
Distal Segment ◽  
Enzymatic Properties ◽  
Gene Encoding ◽  
Mesophyll Cells ◽  
Specific Expression ◽  
Specific Expression Pattern ◽  
High Level

C4 plants are known to be of polyphyletic origin and have evolved independently several times during the evolution of angiosperms. We are interested in understanding the molecular changes that the C4 genes have undergone as they were adapted to their new functions in C4 photosynthesis and are using the C4 phosphoenolpyruvate carboxylase (PEPC) of the genus Flaveria as a model. The PEPCs of F. trinervia (C4) and F. pringlei (C3) are encoded by a gene family that is composed of at least three different gene classes named ppcA, ppcB and ppcC. The C4 PEPC of F. trinervia is encoded by the ppcA gene class and is expressed at high levels only in the mesophyll cells of the leaves. The nearest neighbour to the ppcA gene class of F. trinervia is found in F. pringlei. Comparisons of this pair of orthologous gene classes are used to identify the C4 -specific differences between the enzymatic properties of the ppcA PEPCs and the activities of the ppcA promoters. The two ppcA PEPCs are 96% identical, but differ in the Km for phosphoenolpyruvate (PEP) and their inhibition by malate. Chimerical PEPCs are presently constructed to map the differences in the enzymatic properties of the C4 and C3 PEPC isoforms. To investigate determinants for the C4 specific expression pattern, the 5´ flanking regions of the ppcA1 genes of F. trinervia and F. pringlei were fused to the uidA reporter gene encoding ß-glucuronidase and transformed into the C4 plant F. bidentis and the C3 species tobacco. In F. bidentis, the C4ppcA1 promoter drives a high level of expression of the transgene only in the mesophyll cells, while the C3ppcA1 promoter leads to low levels of expression in leaves, stems and roots. Determinants for the C4 specific expression of the ppcA1 gene of F. trinervia must therefore be located in the 5´-flanking region of this gene. Further analyses showed that two regions, a proximal and a distal segment, are sufficient to generate the C4 specific expression pattern. In tobacco, the C4ppcA1 promoter is preferentially expressed in the palisade parenchyma cells of the leaves. These results indicate that the major events during the evolution of the C4ppcA promoter occurred at the promoter level.

Cloning and Expression Pattern of a Gene Encoding a Putative Plastidic ATP/ADP Transporter from Helianthus tuberosus L.

2005 ◽  
Vol 47 (9) ◽  
pp. 1123-1132 ◽  
Author(s):  
Kun MENG ◽  
Tuan-Jie CHANG ◽  
Xiang LIU ◽  
Song-Biao CHEN ◽  
Yong-Qin WANG ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Cloning And Expression ◽  
Helianthus Tuberosus ◽  
Gene Encoding
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