Regional localisation of 19 brain expressed sequence tags to human chromosome 11 using PCR amplification of somatic cell hybrid DNAs

1995 ◽  
Vol 70 (1-2) ◽  
pp. 71-75 ◽  
Author(s):  
E.M. Slorach ◽  
M.H. Polymeropoulos ◽  
K.L. Evans ◽  
A. Seawright ◽  
J.M. Fletcher ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Cell Hybrid ◽  
Expressed Sequence Tags ◽  
Somatic Cell Hybrid ◽  
Pcr Amplification ◽  
Chromosome 11 ◽  
Hybrid Dnas ◽  
Regional Localisation ◽  
Expressed Sequence

Regional Assignment of 30 Expressed Sequence Tags on Human Chromosome 7 Using a Somatic Cell Hybrid Panel

Genomics ◽  
1995 ◽  
Vol 30 (1) ◽  
pp. 112-114 ◽  
Author(s):  
RESHMA J. PATEL ◽  
JEFFREY T. KEEN ◽  
KARL-HEINZ GRZESCHIK ◽  
WILLIAM C. NIERMAN ◽  
PHILLIP HAYES ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Cell Hybrid ◽  
Expressed Sequence Tags ◽  
Somatic Cell Hybrid ◽  
Chromosome 7 ◽  
Somatic Cell Hybrid Panel ◽  
Expressed Sequence ◽  
Human Chromosome 7 ◽  
Regional Assignment

scholarly journals A myeloid-related sequence that localizes to human chromosome 8q21.1-22

Blood ◽  
1988 ◽  
Vol 71 (6) ◽  
pp. 1713-1719 ◽  
Author(s):  
WM Mars ◽  
P van Tuinen ◽  
HA Drabkin ◽  
JW White ◽  
GF Saunders
Keyword(s):  
Amino Acid ◽  
Human Chromosome ◽  
Somatic Cell ◽  
Southern Blot ◽  
Cell Hybrid ◽  
Somatic Cell Hybrid ◽  
Chromosome 8 ◽  
Nucleotide Sequence Analysis ◽  
Related Sequence ◽  
Hybrid Dnas

Abstract A myeloid-related sequence (mrs) has previously been identified that is highly expressed in selected subpopulations of myeloid leukocytes. Nucleotide sequence analysis indicates that mrs encodes what is apparently a unique 93-amino acid protein that includes an 18-amino acid leader sequence. Hybridization of an mrs cDNA probe to a Southern blot made from somatic cell hybrid DNAs shows 100% concordance with human chromosome 8, thus indicating that mrs localizes to this chromosome. In situ hybridization to metaphase chromosomes further sublocalizes mrs to bands 8q21.1–23 as 58% of the grains displayed on chromosome 8 were clustered in this region. This area encompasses the translocation breakpoint 8q22, which is rearranged in an estimated 18% of patients diagnosed with the M2 subclassification of acute nonlymphocytic leukemia (M2-ANLL). When Southern blot hybridization was performed by using somatic cell hybrid DNAs harboring either a single 8q- or a single 21q+ chromosome from two different patients with M2- ANLL, a signal was only detected in the hybrid containing the 8q- chromosome.

scholarly journals A myeloid-related sequence that localizes to human chromosome 8q21.1-22

Blood ◽  
1988 ◽  
Vol 71 (6) ◽  
pp. 1713-1719
Author(s):  
WM Mars ◽  
P van Tuinen ◽  
HA Drabkin ◽  
JW White ◽  
GF Saunders
Keyword(s):  
Amino Acid ◽  
Human Chromosome ◽  
Somatic Cell ◽  
Southern Blot ◽  
Cell Hybrid ◽  
Somatic Cell Hybrid ◽  
Chromosome 8 ◽  
Nucleotide Sequence Analysis ◽  
Related Sequence ◽  
Hybrid Dnas

A myeloid-related sequence (mrs) has previously been identified that is highly expressed in selected subpopulations of myeloid leukocytes. Nucleotide sequence analysis indicates that mrs encodes what is apparently a unique 93-amino acid protein that includes an 18-amino acid leader sequence. Hybridization of an mrs cDNA probe to a Southern blot made from somatic cell hybrid DNAs shows 100% concordance with human chromosome 8, thus indicating that mrs localizes to this chromosome. In situ hybridization to metaphase chromosomes further sublocalizes mrs to bands 8q21.1–23 as 58% of the grains displayed on chromosome 8 were clustered in this region. This area encompasses the translocation breakpoint 8q22, which is rearranged in an estimated 18% of patients diagnosed with the M2 subclassification of acute nonlymphocytic leukemia (M2-ANLL). When Southern blot hybridization was performed by using somatic cell hybrid DNAs harboring either a single 8q- or a single 21q+ chromosome from two different patients with M2- ANLL, a signal was only detected in the hybrid containing the 8q- chromosome.

Somatic Cell Hybrid Panel and NotI Linking Clones for Physical Mapping of Human Chromosome 3

Genomics ◽  
1994 ◽  
Vol 20 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Ji-Yi Wang ◽  
Eugene R. Zabarovsky ◽  
Cathy Talmadge ◽  
Peter Berglund ◽  
Kate W.K. Chan ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Physical Mapping ◽  
Cell Hybrid ◽  
Somatic Cell Hybrid ◽  
Chromosome 3 ◽  
Somatic Cell Hybrid Panel ◽  
Human Chromosome 3

scholarly journals A somatic cell hybrid panel and DNA probes for physical mapping of human chromosome 7p

Genomics ◽  
1991 ◽  
Vol 11 (3) ◽  
pp. 737-743 ◽  
Author(s):  
A. Vortkamp ◽  
U. Thias ◽  
M. Gessler ◽  
W. Rosenkranz ◽  
P.M. Kroisel ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Physical Mapping ◽  
Cell Hybrid ◽  
Somatic Cell Hybrid ◽  
Dna Probes ◽  
Somatic Cell Hybrid Panel
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