The use of fluorescence in situ hybridization with a β-satellite DNA probe for the detection of acrocentric chromosomes in vanadium-induced micronuclei

1995 ◽  
Vol 69 (3-4) ◽  
pp. 215-219 ◽  
Author(s):  
L. Migliore ◽  
R. Scarpato ◽  
P. Falco
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Satellite Dna ◽  
Dna Probe ◽  
Acrocentric Chromosomes

Analysis by fluorescence in situ hybridization with a mouse gamma satellite DNA probe of isolated micronuclei induced in mice by two clastogens and two spindle poisons

Mutagenesis ◽  
1995 ◽  
Vol 10 (6) ◽  
pp. 513-516 ◽  
Author(s):  
Jorma Mäki-Paakkanen ◽  
Makoto Hayashi ◽  
Takayoshi Suzuki ◽  
Hideyuki Tanabe ◽  
Masamitsu Honma ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Satellite Dna ◽  
Dna Probe

Detection of an i(17q) chromosome by fluorescent in situ hybridization with a chromosome 17 alpha satellite DNA probe

1992 ◽  
Vol 62 (2) ◽  
pp. 140-143 ◽  
Author(s):  
Hitoshi Nakagawa ◽  
Johji Inazawa ◽  
Shinichi Misawa ◽  
Shinji Tanaka ◽  
Teruyuki Takashima ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Satellite Dna ◽  
Fluorescent In Situ Hybridization ◽  
Dna Probe ◽  
Chromosome 17 ◽  
Alpha Satellite ◽  
Alpha Satellite Dna

scholarly journals Presence of 15p Marker D15Z1 on the Short Arm of Acrocentric Chromosomes is Associated with Aneuploid Offspring in Mexican Couples

2019 ◽  
Vol 20 (21) ◽  
pp. 5251 ◽  
Author(s):  
Sandra Ramos ◽  
Rebeca Rodríguez ◽  
Oscar Castro ◽  
Patricia Grether ◽  
Bertha Molina ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
High Frequency ◽  
Trisomy 21 ◽  
Mexican Population ◽  
The Other ◽  
Human Populations ◽  
Nucleolar Organization ◽  
Acrocentric Chromosomes

Variation in the location of the 15p region D15Z1 is recognized as a polymorphism in several human populations. We used high-stringency Fluorescence In Situ Hybridization (FISH) to detect D15Z1 in a Mexican cohort. Here, we report the presence of extra D15Z1 sequences on the p-arm of acrocentric chromosomes other than 15 in two groups of Mexican couples, one with healthy offspring (n = 75) and the other with aneuploid offspring (n = 87), mainly trisomy 21. The additional D15Z1 polymorphism was significantly increased in individuals with aneuploid offspring (26.4%), in comparison to individuals with healthy offspring (14%). The most frequent acceptor chromosome of D15Z1 was chromosome 13p, followed by 14p, and finally, 21p. Our results show an overall frequency of 21.6% of this polymorphism in the Mexican population and suggest that its presence might be associated with the mis-segregation of other acrocentric chromosomes and aneuploid offspring. The high frequency of the polymorphism of the D15Z1 sequence on acrocentric chromosomes other than 15 suggests a sequence homogenization of the acrocentric p arms, related to the important function of the centromere and the nucleolar organization region, which flank satellite III DNA.

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