High resolution ordering of DNA markers by multi-color fluorescent in situ hybridization of prophase chromosomes

1994 ◽  
Vol 65 (1-2) ◽  
pp. 130-135 ◽  
Author(s):  
J. Inazawa ◽  
T. Ariyama ◽  
T. Tokino ◽  
A. Tanigami ◽  
Y. Nakamura ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
High Resolution ◽  
Dna Markers ◽  
Fluorescent In Situ Hybridization

High-Resolution Fluorescent In Situ Hybridization of Drosophila Embryos and Tissues

2008 ◽  
Vol 2008 (6) ◽  
pp. pdb.prot5019-pdb.prot5019 ◽  
Author(s):  
E. Lecuyer ◽  
A. S. Necakov ◽  
L. Caceres ◽  
H. M. Krause
Keyword(s):  
In Situ Hybridization ◽  
High Resolution ◽  
Fluorescent In Situ Hybridization ◽  
Drosophila Embryos

scholarly journals Mapping of the nu gene using congenic nude strains and in situ hybridization.

1992 ◽  
Vol 175 (3) ◽  
pp. 873-876 ◽  
Author(s):  
Y Takahashi ◽  
A Shimizu ◽  
T Sakai ◽  
Y Endo ◽  
N Osawa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
High Resolution ◽  
Mouse Chromosome ◽  
Nude Mouse ◽  
Physical Mapping ◽  
Dna Markers ◽  
Chromosomal Location ◽  
Chromosome 11 ◽  
Mouse Chromosome 11

The chromosomal location of the nu gene, which is responsible for hairlessness and athymus, was determined using six DNA markers (interleukin 3 [Il-3], Myhs, Acrb, Evi-2, Mpo, and Hox-2) on mouse chromosome 11. We constructed the high-resolution physical mapping of the six DNA markers on chromosome 11 by in situ hybridization using fluorescence-labeled cosmid probes. The results indicate the order of centromere-(41cM)-Il-3-(3cM)-Myhs- (4cM)-Acrb-(6cM)-Evi-2-(3cM)-Mpo-(5cM)- Hox-2. We have used congenic nude strains and examined which of the six DNA markers were derived from the original nude mouse. We found the Evi-2 locus is linked to the nu gene in all the informative, independent congenic nude strains. From these data, we could estimate the location of the nu gene, not only genetically but also physically within a region that spans approximately 17 megabases (9 cM) between the Acrb and Mpo genes.

scholarly journals Combined confocal and wide-field high-resolution cytometry of fluorescent in situ hybridization-stained cells

Cytometry ◽  
2001 ◽  
Vol 45 (1) ◽  
pp. 1-12 ◽  
Author(s):  
M. Kozubek ◽  
S. Kozubek ◽  
E. Luk�?ov� ◽  
E. B�rtov� ◽  
M. Skaln�kov� ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
High Resolution ◽  
Fluorescent In Situ Hybridization ◽  
Wide Field

Ultrastructural analysis of the spatial distribution of MRNA

1992 ◽  
Vol 50 (1) ◽  
pp. 552-553
Author(s):  
Gary Bassell ◽  
Robert H. Singer
Keyword(s):  
Electron Microscopy ◽  
Spatial Distribution ◽  
In Situ Hybridization ◽  
High Resolution ◽  
Nucleic Acids ◽  
Colloidal Gold ◽  
Dna Probe ◽  
Nucleotide Analogs ◽  
Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

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