Chromosome assignment of human brain expressed sequence tags (ESTs) by analyzing f luorescently labeled PCR products from hybrid cell panels

1994 ◽  
Vol 65 (1-2) ◽  
pp. 86-91 ◽  
Author(s):  
A.S. Durkin ◽  
W.C. Nierman ◽  
H. Zoghbi ◽  
C. Jones ◽  
C.A. Kozak ◽  
...  
Keyword(s):  
Human Brain ◽  
Expressed Sequence Tags ◽  
Hybrid Cell ◽  
Chromosome Assignment ◽  
Pcr Products ◽  
Expressed Sequence

Development of an EST-SSR marker in Panax ginseng

2008 ◽  
Vol 5 (2) ◽  
pp. 175-181 ◽  
Author(s):  
Yang Cheng-Jun ◽  
Wang Jun ◽  
Mu Li-Qiang ◽  
Li Shao-Chen ◽  
Liu Guan-Jun ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Expressed Sequence Tags ◽  
Genomic Dna ◽  
Ssr Marker ◽  
Panax Quinquefolius ◽  
Chain Reaction ◽  
Acanthopanax Senticosus ◽  
Pcr Products ◽  
Polymerase Chain ◽  
Expressed Sequence

AbstractA total of 791 microsatellites (SSRs) were isolated from 7055 Panax ginseng expressed sequence tags (ESTs). According to primer design criteria, 68 primer pairs for EST-SSR were designed. Under an appropriate polymerase chain reaction (PCR) system, all EST-SSR primer pairs were screened against genomic DNA of Ji'anchangbo and Fusong'ermaya from Panax ginseng, and 43 EST-SSR primer pairs out of the above 68 resulted in PCR products. Then, all 43 pairs were detected in nine P. ginseng, two Panax quinquefolius and two Acanthopanax senticosus cultivars for polymorphisms, and 26 pairs (60.47%) were found to be polymorphic, accounting for 38.23% of the total number of designed primer pairs. These results demonstrate the possibility of developing EST-SSR markers using P. ginseng ESTs.

ANALYSIS OF EXPRESSED SEQUENCE TAGS FROM HUMAN BRAIN CDNAS

1993 ◽  
Author(s):  
Mark D. Adams ◽  
Anthony R. Kerlavage ◽  
Mark Dubnick ◽  
Ruben F. Moreno ◽  
Chris Fields ◽  
...  
Keyword(s):  
Human Brain ◽  
Expressed Sequence Tags ◽  
Expressed Sequence

Apple SSRs present in coding and noncoding regions of expressed sequence tags show differences in transferability to other fruit species in Rosaceae

2013 ◽  
Vol 93 (2) ◽  
pp. 183-190 ◽  
Author(s):  
Ying Zhou ◽  
Jing Li ◽  
Schuyler S. Korban ◽  
Yuepeng Han
Keyword(s):  
Expressed Sequence Tags ◽  
Tree Species ◽  
Fruit Trees ◽  
Fruit Tree ◽  
Noncoding Regions ◽  
Fruit Species ◽  
Pcr Products ◽  
Rosaceae Family ◽  
Expressed Sequence ◽  
Simple Sequence

Zhou, Y., Li, J., Korban, S. S. and Han, Y. 2013. Apple SSRs present in coding and noncoding regions of expressed sequence tags show differences in transferability to other fruit species in Rosaceae. Can. J. Plant Sci. 93: 183–190. Simple sequence repeat markers derived from expressed sequence tags (ESTs) are referred to as eSSRs. To develop molecular markers for non-model plants in Rosaceae, we investigated the transferability of apple eSSRs across seven fruit trees, belonging to four genera and 11 species of the Rosaceae family, including peach, quince, pear, loquat, apricot, cherry, and plum. Of the 98 apple eSSRs tested, 86 successfully amplified PCR products in at least one of the fruit tree species. Five apple eSSRs produced amplicons in more than five fruit tree species, and were deemed as a widely transferable Rosaceae marker set. Frequency of transferability of apple eSSRs across all seven fruit trees of Rosaceae varied widely among genera and species, with an average transferability of 29.0%. Overall, apple eSSRs transferred more easily to peach and pear than to plum and loquat. Interestingly, apple eSSRs present in coding sequences (CDS) showed higher levels of transferability to other fruit trees than those present in noncoding or untranslated regions (UTRs). Interestingly, apple eSSRs present in 5'UTRs showed lower frequencies of transfer than those present in 3'UTRs. The latter finding suggested that 5'UTRs might have diverged more rapidly than 3'UTRs in Rosaceae.

scholarly journals Expressed Sequence Tags from Roots and Nodule Primordia of Lotus japonicus Infected with Mesorhizobium loti

2002 ◽  
Vol 15 (4) ◽  
pp. 376-379 ◽  
Author(s):  
Carsten Poulsen ◽  
Lone Pødenphant
Keyword(s):  
Expressed Sequence Tags ◽  
Messenger Rna ◽  
Root Nodules ◽  
Lotus Japonicus ◽  
Cdna Expression Library ◽  
Expression Library ◽  
Mesorhizobium Loti ◽  
Pcr Products ◽  
Plant Genes ◽  
Expressed Sequence

Messenger RNA from young Lotus japonicus roots carrying root nodule primordia appearing after inoculation with Mesorhizobium loti bacteria were used to construct a cDNA expression library. Single-pass sequencing employing colony-polymerase chain reaction (PCR) and analysis of PCR products established a total of 2,397 new expressed sequence tags (ESTs). We have putatively identified 1,236 known and 484 hypothetical proteins coded by the corresponding mRNAs. The remaining cDNAs are unknown (316) or redundant overlapping cDNAs (361). We hope that this batch of ESTs will assist in the recognition of plant genes involved during development of nitrogen-fixing root nodules.

3,400 new expressed sequence tags identify diversity of transcripts in human brain

1993 ◽  
Vol 4 (3) ◽  
pp. 256-267 ◽  
Author(s):  
Mark D. Adams ◽  
Anthony R. Kerlavage ◽  
Chris Fields ◽  
J. Craig Venter
Keyword(s):  
Human Brain ◽  
Expressed Sequence Tags ◽  
Expressed Sequence

Chromosome assignment of 115 expressed sequence tags (ESTs) from human skeletal muscle

1997 ◽  
Vol 76 (3-4) ◽  
pp. 144-152 ◽  
Author(s):  
T. Muraro ◽  
D. Stephan ◽  
N. Tiso ◽  
R. Zimbello ◽  
G.A. Danieli ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Expressed Sequence Tags ◽  
Human Skeletal Muscle ◽  
Chromosome Assignment ◽  
Expressed Sequence
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