Mapping of the human cardiac Na+/Ca2+ exchanger gene (IMCX1) by fluorescent in situ hybridization to chromosome region 2p22→p23

1993 ◽  
Vol 63 (3) ◽  
pp. 192-193 ◽  
Author(s):  
L.D. McDaniel ◽  
W.J. Lederer ◽  
P. Kofuji ◽  
D.H. Schulze ◽  
R. Kieval ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Chromosome Region

Localization of the repetitive telomeric sequence (TTAGGG)n in four salmonid species

Genome ◽  
1996 ◽  
Vol 39 (5) ◽  
pp. 1035-1038 ◽  
Author(s):  
M. Abuín ◽  
P. Martínez ◽  
L. Sánchez
Keyword(s):  
In Situ Hybridization ◽  
Salmo Salar ◽  
Fluorescent In Situ Hybridization ◽  
Salmo Trutta ◽  
Chromosome Region ◽  
Telomeric Sequence ◽  
Evolutionary Mechanism ◽  
Salmonid Species ◽  
Telomeric Probe

We have analyzed the localization of the highly conserved telomeric sequence (TTAGGG)n in four salmonid species, two of the genus Salmo (Salmo trutta and Salmo salar) and two of the genus Oncorhynchus (Onchorhynchus mykiss and Onchorhynchus kisutch), by fluorescent in situ hybridization. As expected, the hybridization signal was mostly localized at the telomeres of all chromosomes in the four species. Two species evidenced special hybridization sites with the telomeric probe: (i) interstitial heterochromatic blocks in particular long chromosomes in S. salar, this observation supports tandem fusions as the karyotypic evolutionary mechanism leading to the formation of the long acrocentric and submetacentric chromosomes in the karyotype of S. salar; (ii) the whole NOR region in O. mykiss; this observation suggests that the (TTAGGG)n sequence is scattered all along this chromosome region. Key words : Salmo, Oncorhynchus, telomeres, in situ hybridization, evolution.

1796: Analysis of Chromosomal Aneuploidy in Patients with Oligoasthenoteratozoospermia(OAT) Using Fluorescent in-situ Hybridization

2007 ◽  
Vol 177 (4S) ◽  
pp. 596-597
Author(s):  
Joseph P. Alukal ◽  
Bobby B. Najari ◽  
Wilson Chuang ◽  
Lata Murthy ◽  
Monica Lopez-Perdomo ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Chromosomal Aneuploidy

Fluorescent in situ hybridization (FISH): A useful diagnostic tool for childhood conjunctival melanoma

2021 ◽  
pp. 112067212110307
Author(s):  
Raquel María Moral ◽  
Carlos Monteagudo ◽  
Javier Muriel ◽  
Lucía Moreno ◽  
Ana María Peiró
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Pediatric Population ◽  
Diagnostic Technique ◽  
Histopathological Diagnosis ◽  
Fish Analysis ◽  
Conjunctival Melanoma ◽  
Adequate Treatment ◽  
First Case

Introduction: Conjunctival melanoma is extremely rare in children and has low rates of resolution. Definitive histopathological diagnosis based exclusively on microscopic findings is sometimes difficult. Thus, early diagnosis and adequate treatment are essential to improve clinical outcomes. Clinical case: We present the first case in which the fluorescent in situ hybridization (FISH) diagnostic technique was applied to a 10-year-old boy initially suspected of having amelanotic nevi in his right eye. Based on the 65% of tumor cells with 11q13 (CCND1) copy number gain and 33% with 6p25 (RREB1) gain as measured by the FISH analysis, and on supporting histopathological findings, the diagnosis of conjunctival melanoma could be made. Following a larger re-excision, adjuvant therapy with Mitomycin C (MMC), cryotherapy and an amniotic membrane graft, the patient has remained disease-free during 9 years of long-term follow-up. Case discussion: Every ophthalmologist should remember to consider and not forget the possibility of using FISH analyses during the differential diagnosis of any suspicious conjunctival lesions. Genetic techniques, such as FISH, have led to great advances in the classification of ambiguous lesions. Evidence-based guidelines for diagnosing conjunctival melanoma in the pediatric population are needed to determine the most appropriate strategy for this age group.

scholarly journals Combination of Direct Viable Count and Fluorescent In Situ Hybridization (DVC-FISH) as a Potential Method for Identifying Viable Vibrio parahaemolyticus in Oysters and Mussels

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1502
Author(s):  
Jorge García-Hernández ◽  
Manuel Hernández ◽  
Yolanda Moreno
Keyword(s):  
In Situ Hybridization ◽  
Vibrio Parahaemolyticus ◽  
Fluorescent In Situ Hybridization ◽  
Potential Method ◽  
Viable Count ◽  
Hybridization Technique ◽  
Fish Technique ◽  
Viable Cells

Vibrio parahaemolyticus is a human food-borne pathogen with the ability to enter the food chain. It is able to acquire a viable, non-cultivable state (VBNC), which is not detected by traditional methods. The combination of the direct viable count method and a fluorescent in situ hybridization technique (DVC-FISH) makes it possible to detect microorganisms that can present VBNC forms in complex samples The optimization of the in vitro DVC-FISH technique for V. parahaemolyticus was carried out. The selected antibiotic was ciprofloxacin at a concentration of 0.75 μg/mL with an incubation time in DVC broth of 5 h. The DVC-FISH technique and the traditional plate culture were applied to detect and quantify the viable cells of the affected pathogen in artificially contaminated food matrices at different temperatures. The results obtained showed that low temperatures produced an important logarithmic decrease of V. parahaemolyticus, while at 22 °C, it proliferated rapidly. The DVC-FISH technique proved to be a useful tool for the detection and quantification of V. parahaemolyticus in the two seafood matrices of oysters and mussels. This is the first study in which this technique has been developed to detect viable cells for this microorganism.

Catalyzed reporter deposition-fluorescent in situ hybridization (CARD-FISH) detection of Dehalococcoides

2008 ◽  
Vol 73 (2) ◽  
pp. 142-147 ◽  
Author(s):  
J.A. Dijk ◽  
P. Breugelmans ◽  
J. Philips ◽  
P.J. Haest ◽  
E. Smolders ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Fish Detection ◽  
Catalyzed Reporter Deposition ◽  
Card Fish

scholarly journals Evaluation of MET gene status in malignant mesothelioma by fluorescent in situ hybridization

2016 ◽  
Vol 27 ◽  
pp. iv8
Author(s):  
A. Vigani ◽  
S. Salvi ◽  
S. Varesano ◽  
S. Boccardo ◽  
P. Ferro ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Malignant Mesothelioma ◽  
Fluorescent In Situ Hybridization ◽  
Gene Status
Sign in / Sign up

Export Citation Format

Share Document