Localization of the horse (Equus caballus) α-globin gene complex to chromosome 13 by luorescence in situ hybridization

1993 ◽  
Vol 62 (2-3) ◽  
pp. 136-138 ◽  
Author(s):  
E.A. Oakenfull ◽  
V.J. Buckle ◽  
J.B. Clegg
Keyword(s):  
In Situ Hybridization ◽  
Globin Gene ◽  
Gene Complex ◽  
Chromosome 13 ◽  
Alpha Globin

In situ hybridization reveals co-expression of embryonic and adult alpha globin genes in the earliest murine erythrocyte progenitors

Development ◽  
1992 ◽  
Vol 116 (4) ◽  
pp. 1041-1049 ◽  
Author(s):  
A. Leder ◽  
A. Kuo ◽  
M.M. Shen ◽  
P. Leder
Keyword(s):  
In Situ Hybridization ◽  
Embryonic Development ◽  
Fetal Liver ◽  
Globin Gene ◽  
Yolk Sac ◽  
Globin Genes ◽  
Globin Mrna ◽  
Mature Adult ◽  
Alpha Globin

Murine erythropoiesis begins with the formation of primitive red blood cells in the blood islands of the embryonic yolk sac on day 7.5 of gestation. By analogy to human erythropoiesis, it has been thought that there is a gradual switch from the exclusive expression of the embryonic alpha-like globin (zeta) to the mature adult form (alpha) in these early mouse cells. We have used in situ hybridization to assess expression of these two globin genes during embryonic development. In contrast to what might have been expected, we find that there is simultaneous expression of both zeta and alpha genes from the very onset of erythropoiesis in the yolk sac. At no time could we detect expression of embryonic zeta globin mRNA without concomitant expression of adult alpha globin mRNA. Indeed, adult alpha transcripts exceed those of embryonic zeta in the earliest red cell precursors. Moreover, the pattern of hybridization reveals co-expression of both genes within the same cells. Even in the fetal liver, which supersedes the yolk sac as the major site of murine fetal erythropoiesis, there is a brief co-expression of zeta and alpha genes followed by the exclusive expression of the adult alpha genes. These data indicate an important difference in hematopoietic ontogeny between mouse and that of human, where zeta expression precedes that of alpha. In addition to resolving the embryonic expression of these globin genes, our results suggest that the embryonic alpha-like globin gene zeta may be physiologically redundant, even during the earliest stages of embryonic development.

scholarly journals Localization of the Ceruloplasmin (CP) Gene to the q32-q33 Bands of Chromosome 13 in Pigs by in Situ Hybridization

Hereditas ◽  
2004 ◽  
Vol 119 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Renuka Chaudhary ◽  
Bhanu P. Chowdhary ◽  
Ingrid Harbitz ◽  
Ingemar Gustavsson
Keyword(s):  
In Situ Hybridization ◽  
Chromosome 13 ◽  
Cp Gene

Localization of specific mRNAs in Xenopus embryos by whole-mount in situ hybridization

Development ◽  
1990 ◽  
Vol 110 (2) ◽  
pp. 325-330 ◽  
Author(s):  
A. Hemmati-Brivanlou ◽  
D. Frank ◽  
M.E. Bolce ◽  
B.D. Brown ◽  
H.L. Sive ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Antisense Rna ◽  
Muscle Actin ◽  
Xenopus Embryos ◽  
Whole Mount ◽  
Alpha Globin ◽  
Specific Mrnas ◽  
Protein Rich ◽  
Hatching Gland

We have adapted a non-radioactive technique to detect localized mRNAs in whole-mount Xenopus embryos. Synthetic antisense RNA transcribed in the presence of digoxygenin-UTP is used as a probe and is detected via an anti-digoxygenin antibody. We show that localized mRNAs can be detected from late gastrula to tadpole stages and that high as well as low abundance RNAs can be detected. The method was tested on muscle actin and alpha-globin RNAs, whose localization has previously been characterized. In addition, we used the method to determine the distribution of XA-1 RNA, an anterior ectoderm-specific RNA, which we show is expressed in the periphery of the cement gland as well as in the region of the hatching gland. The sequence of an XA-1 cDNA predicts a protein rich in proline and histidine.

Assignment, by in Situ hybridization, of the growth arrest-specific gene, Gas-1, to mouse chromosome 13, bands B3–C2

Genomics ◽  
1992 ◽  
Vol 14 (2) ◽  
pp. 548-549 ◽  
Author(s):  
G.C. Webb ◽  
P.A. Cowled ◽  
A. Evdokiou ◽  
J.H. Ford ◽  
I.J. Forbes
Keyword(s):  
In Situ Hybridization ◽  
Mouse Chromosome ◽  
Growth Arrest ◽  
Specific Gene ◽  
Chromosome 13

Regional Localization of 32 NotI-HindIII Fragments from a Human Chromosome 13 Library by a Somatic Cell Hybrid Panel and in Situ Hybridization

Genomics ◽  
1993 ◽  
Vol 16 (2) ◽  
pp. 355-360 ◽  
Author(s):  
Dorothy Warburton ◽  
Ming-Tsung Yu ◽  
Umadevi Tantravahi ◽  
Carolyn Lee ◽  
Eftihia Cayanis ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Chromosome ◽  
Somatic Cell ◽  
Cell Hybrid ◽  
Somatic Cell Hybrid ◽  
Somatic Cell Hybrid Panel ◽  
Regional Localization ◽  
Chromosome 13

Assignments of polymorphic loci on chromosome 13 refined by in situ hybridization

Genomics ◽  
1992 ◽  
Vol 12 (4) ◽  
pp. 852-853 ◽  
Author(s):  
Christine M. Morris ◽  
Peter H. Fitzgerald
Keyword(s):  
In Situ Hybridization ◽  
Chromosome 13 ◽  
Polymorphic Loci
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