Detection of human chromosomes in somatic cell hybrids by PCR analysis

1993 ◽  
Vol 62 (1) ◽  
pp. 1-4 ◽  
Author(s):  
P. Tripputi ◽  
G. Bacciocchi ◽  
R.S. Accolla ◽  
G. Mantero ◽  
G. DeBellis ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Pcr Analysis ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Localization of genes on human chromosomes by studies of human-Chinese hamster somatic cell hybrids

1973 ◽  
Vol 20 (3) ◽  
pp. 195-202 ◽  
Author(s):  
Ans Jongsma ◽  
Harry Someren ◽  
Andries Westerveld ◽  
Ann Hagemeijer ◽  
Peter Pearson
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Mouse-Human Somatic Cell Hybrids: Loss of Mouse and Human Chromosomes

1998 ◽  
Vol 24 (3) ◽  
pp. 165-171 ◽  
Author(s):  
Xianghong Wang ◽  
Margaret Fox ◽  
Susan Povey ◽  
John R.W. Masters
Keyword(s):  
Somatic Cell ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Human Somatic Cell ◽  
Cell Hybrids

Regional assignment of the human acid sphingomyelinase gene (SMPD1) by PCR analysis of somatic cell hybrids and in situ hybridization to 11p15.1→p15.4

Genomics ◽  
1991 ◽  
Vol 9 (2) ◽  
pp. 229-234 ◽  
Author(s):  
Lygia da Veiga Pereira ◽  
Robert J. Desnick ◽  
David A. Adler ◽  
Christine M. Disteche ◽  
Edward H. Schuchman
Keyword(s):  
In Situ Hybridization ◽  
Somatic Cell ◽  
Acid Sphingomyelinase ◽  
Pcr Analysis ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Human Acid ◽  
Regional Assignment

scholarly journals Multiple human beta interferon genes.

1982 ◽  
Vol 156 (3) ◽  
pp. 744-755 ◽  
Author(s):  
A D Sagar ◽  
P B Sehgal ◽  
D L Slate ◽  
F H Ruddle
Keyword(s):  
Somatic Cell ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Diploid Fibroblasts ◽  
Mrna Species ◽  
Human Diploid Fibroblasts ◽  
Beta Interferon ◽  
Cell Hybrids ◽  
Marked Cell ◽  
Mouse Somatic Cell

Analysis of human beta interferon (IFN) mRNA preparations obtained from poly(I) . poly (C)-induced human diploid fibroblasts (FS-4) and from several similarly induced human-mouse somatic cell hybrids by electrophoresis through agarose-CH3HgOH tube gels led to the detection of at least five translationally active human IFN-beta mRNA species. The results obtained are consistent with the existence of IFN-beta genes on different human chromosomes. Marked cell-dependent variability in the expression of these IFN mRNA species was observed.

PCR primers for human chromosomes: Reagents for the rapid analysis of somatic cell hybrids

Genomics ◽  
1991 ◽  
Vol 9 (3) ◽  
pp. 511-516 ◽  
Author(s):  
S. Theune ◽  
J. Fung ◽  
S. Todd ◽  
A.Y. Sakaguchi ◽  
S.L. Naylor
Keyword(s):  
Somatic Cell ◽  
Pcr Primers ◽  
Rapid Analysis ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Human beta interferon gene localization and expression in somatic cell hybrids

1982 ◽  
Vol 2 (5) ◽  
pp. 564-570
Author(s):  
P M Pitha ◽  
D L Slate ◽  
N B Raj ◽  
F H Ruddle
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Cdna Clone ◽  
Sequence Homology ◽  
Chromosome 9 ◽  
Gene Localization ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Interferon Gene

The human fibroblast interferon gene beta 1 was mapped to human chromosome 9. Sequence homology with a beta 1 cDNA clone was detected in both genomic DNA and induced mRNA of human/mouse or human/hamster somatic cell hybrids containing human chromosome 9, but not in lines lacking this chromosome or those retaining a complex translocation involving chromosomes 9 and 11. Interferon mRNA that did not share sequence homology with the beta 1 cDNA clone was detected in lines containing human chromosomes 2 and 5 but lacking chromosome 9, suggesting the presence of other unlinked interferon sequences in the human genome.

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