In situ hybridisation localises the gene for the major intrinsic protein of eye lens fibre cell membranes to human chromosome 12q14

1992 ◽  
Vol 61 (1) ◽  
pp. 8-9 ◽  
Author(s):  
C.S. Griffin ◽  
A. Shiels
Keyword(s):  
Human Chromosome ◽  
Cell Membranes ◽  
In Situ Hybridisation ◽  
Eye Lens ◽  
Major Intrinsic Protein ◽  
Intrinsic Protein ◽  
Fibre Cell ◽  
Lens Fibre ◽  
Lens Fibre Cell

scholarly journals Studies on lipids and the activity of Na,K-ATPase in lens fibre cells

1996 ◽  
Vol 314 (3) ◽  
pp. 961-967 ◽  
Author(s):  
William L. DEAN ◽  
Nicholas A. DELAMERE ◽  
Douglas BORCHMAN ◽  
Amy E. MOSELEY ◽  
Rajesh P. AHUJA
Keyword(s):  
Epithelial Cell ◽  
Cell Membrane ◽  
Atpase Activity ◽  
Lipid Composition ◽  
Cell Membranes ◽  
Lens Epithelium ◽  
Membrane Material ◽  
Fibre Cell ◽  
Lens Fibre ◽  
Lens Fibre Cell

Na,K-ATPase was studied in the two cell types that make up the lens of the eye. Membrane material was isolated from lens fibre cells, which make up the bulk of the lens cell mass, and also from lens epithelial cells, which are present only as a monolayer on the anterior lens surface. Judged by immunoblotting, greater amounts of Na,K-ATPase α1 and β1 polypeptides were found in fibre cell membrane material than in epithelial cell membrane material. However, the Na,K-ATPase activity in epithelial cell membrane material was 20 times that measured in fibre cell membrane material. In 86Rb uptake experiments with intact lenses, ouabain-inhibitable 86Rb uptake was observed for lens epithelium but not for lens fibres. These findings are consistent with a low Na,K-ATPase activity in lens fibre cells even though these cells express a considerable amount of Na,K-ATPase α1 and β1 polypeptides. The lipid composition of lens fibre cell membranes causes them to be more ordered than epithelial cell membranes; this was confirmed by measurements of the infrared CH2 symmetric stretching band frequency. Because lipid composition can influence Na,K-ATPase activity, experiments were conducted to determine whether the activity of Na,K-ATPase α1β1 is inhibited by lens fibre lipid. However, no significant difference in Na,K-ATPase activity was detected when Na,K-ATPase α1β1 was purified from rabbit kidney and then reconstituted with lipid that had been isolated from either lens epithelium or lens fibre cells. These studies indicate that lens fibre cells contain both Na,K-ATPase α1 and β1 polypeptides but have low Na,K-ATPase activity. However, the results do not support the notion that this is due to the lipid composition of lens fibre cell membranes.

Bfsp2 mutation found in mouse 129 strains causes the loss of CP49 and induces vimentin-dependent changes in the lens fibre cell cytoskeleton

2004 ◽  
Vol 78 (1) ◽  
pp. 109-123 ◽  
Author(s):  
Aileen Sandilands ◽  
Xin Wang ◽  
Aileen M Hutcheson ◽  
John James ◽  
Alan R Prescott ◽  
...  
Keyword(s):  
Fibre Cell ◽  
Cell Cytoskeleton ◽  
Lens Fibre ◽  
Lens Fibre Cell

Polypeptides of the lens fibre cell intracellular matrix

1980 ◽  
Vol 36 (4) ◽  
pp. 416-418 ◽  
Author(s):  
M. Katar ◽  
W. -K. Lo ◽  
M. Nagpal ◽  
H. Maisel
Keyword(s):  
Fibre Cell ◽  
Lens Fibre ◽  
Lens Fibre Cell
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