Characterization of a human chromosome 8 cosmid library constructed from flow-sorted chromosomes

1992 ◽  
Vol 59 (4) ◽  
pp. 243-247 ◽  
Author(s):  
S. Wood ◽  
M. Schertzer ◽  
H. Drabkin ◽  
D. Patterson ◽  
J.L. Longmire ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Chromosome 8 ◽  
Cosmid Library

Generation and characterization of a human chromosome 9 cosmid library

1992 ◽  
Vol 18 (3) ◽  
pp. 269-284 ◽  
Author(s):  
Sharon L. Graw ◽  
Alan J. Buckler ◽  
Deborah E. Britt ◽  
Cynthia L. Jackson ◽  
Domenica Taruscio ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Chromosome 9 ◽  
Cosmid Library

Characterization of a flow-sorted human chromosome 10 cosmid library by FISH

1996 ◽  
Vol 74 (4) ◽  
pp. 266-271 ◽  
Author(s):  
N.S.-F. Ma ◽  
C. Zheng ◽  
Y. Benchekroun ◽  
L.L. Deaven ◽  
J.L. Longmire ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Cosmid Library ◽  
Chromosome 10

Characterization of a human chromosome 4 flow-sorted cosmid library

1994 ◽  
Vol 65 (4) ◽  
pp. 238-242 ◽  
Author(s):  
O. Riess ◽  
I. Siedlaczck ◽  
S. Kredtke ◽  
G. Melmer ◽  
J.T. Epplen ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Cosmid Library ◽  
Chromosome 4 ◽  
Human Chromosome 4

scholarly journals Gene Cloning and Molecular Characterization of a Two-Enzyme System Catalyzing the Oxidative Detoxification of β-Endosulfan

2002 ◽  
Vol 68 (12) ◽  
pp. 6237-6245 ◽  
Author(s):  
Tara D. Sutherland ◽  
Irene Horne ◽  
Robyn J. Russell ◽  
John G. Oakeshott
Keyword(s):  
Shuttle Vector ◽  
Open Reading Frame ◽  
Cosmid Library ◽  
Flavin Mononucleotide ◽  
Flavin Reductase ◽  
Reading Frame ◽  
Reductase Gene ◽  
Degradation Activity ◽  
Layer Chromatography

ABSTRACT The gram-positive bacterium Mycobacterium sp. strain ESD is able to use the cyclodiene insecticide endosulfan as a source of sulfur for growth. This activity is dependent on the absence of sulfite or sulfate in the growth medium. A cosmid library of strain ESD DNA was constructed in a Mycobacterium-Escherichia coli shuttle vector and screened for endosulfan-degrading activity in Mycobacterium smegmatis, a species that does not degrade endosulfan. Using this method, we identified a single cosmid that conferred sulfur-dependent endosulfan-degrading activity on the host strain. An open reading frame (esd) was identified within this cosmid that, when expressed behind a constitutive promoter in a mycobacterial expression vector, conferred sulfite- and sulfate-independent β-endosulfan degradation activity on the recombinant strain. The translation product of this gene (Esd) had up to 50% sequence identity with an unusual family of monooxygenase enzymes that use reduced flavins, provided by a separate flavin reductase enzyme, as cosubstrates. An additional partial open reading frame was located upstream of the Esd gene that had sequence homology to the same monooxygenase family. A flavin reductase gene, identified in the M. smegmatis genome, was cloned, expressed, and used to provide reduced flavin mononucleotide for Esd in enzyme assays. Thin-layer chromatography and gas chromatography analyses of the enzyme assay mixtures revealed the disappearance of β-endosulfan and the appearance of the endosulfan metabolites, endosulfan monoaldehyde and endosulfan hydroxyether. This suggests that Esd catalyzes the oxygenation of β-endosulfan to endosulfan monoaldehyde and endosulfan hydroxyether. Esd did not degrade either α-endosulfan or the metabolite of endosulfan, endosulfan sulfate.

Molecular cloning and characterization of gene for Golgi-localized syntaphilin-related protein on human chromosome 8q23

Gene ◽  
2005 ◽  
Vol 344 ◽  
pp. 259-271 ◽  
Author(s):  
Eishi Funakoshi ◽  
Kin-ya Nakagawa ◽  
Ayako Hamano ◽  
Takamitsu Hori ◽  
Atsushi Shimizu ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Molecular Cloning ◽  
Related Protein

Construction and Characterization of a Partial Library of Yeast Artificial Chromosomes from Human Chromosome 21

1991 ◽  
Vol 10 (4) ◽  
pp. 301-310 ◽  
Author(s):  
M. BELLIS ◽  
A. GÉRARD ◽  
J.P. CHARLIEU ◽  
B. MARÇAIS ◽  
M.E. BRUN ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Chromosome 21 ◽  
Artificial Chromosomes ◽  
Human Chromosome 21 ◽  
Yeast Artificial Chromosomes

Characterization of ZNF333, a novel double KRAB domain containing zinc finger gene on human chromosome 19p13.1

2002 ◽  
Vol 1577 (1) ◽  
pp. 121-125 ◽  
Author(s):  
Yong Tian ◽  
Guido J Breedveld ◽  
Shangzhi Huang ◽  
Ben A Oostra ◽  
Peter Heutink ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Zinc Finger ◽  
Zinc Finger Gene ◽  
Krab Domain
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