Localization of the gene encoding insulin-like growth factor I on mouse Chromosome 10

1991 ◽  
Vol 56 (1) ◽  
pp. 57-58 ◽  
Author(s):  
B.A. Taylor ◽  
D. Grieco
Keyword(s):  
Growth Factor ◽  
Mouse Chromosome ◽  
Insulin Like Growth Factor ◽  
Chromosome 10 ◽  
Gene Encoding ◽  
Factor I ◽  
Growth Factor I

The human gene encoding insulin-like growth factor I is located on chromosome 12

1985 ◽  
Vol 69 (2) ◽  
pp. 157-160 ◽  
Author(s):  
J. W. M. Höppener ◽  
P. de Pagter-Holthuizen ◽  
A. H. M. Geurts van Kessel ◽  
M. Jansen ◽  
S. D. Kittur ◽  
...  
Keyword(s):  
Growth Factor ◽  
Human Gene ◽  
Chromosome 12 ◽  
Insulin Like Growth Factor ◽  
Gene Encoding ◽  
Factor I ◽  
Growth Factor I

scholarly journals Enhanced Production of Insulin-Like Growth Factor I Fusion Protein in Escherichia coli by Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling

2003 ◽  
Vol 69 (8) ◽  
pp. 4737-4742 ◽  
Author(s):  
Jong Hyun Choi ◽  
Sang Jun Lee ◽  
Seok Jae Lee ◽  
Sang Yup Lee
Keyword(s):  
Escherichia Coli ◽  
Growth Factor ◽  
Fusion Protein ◽  
Transcriptome Profiling ◽  
Insulin Like Growth Factor ◽  
Gene Encoding ◽  
Factor I ◽  
Enhanced Production ◽  
Igf I ◽  
Growth Factor I

ABSTRACT The transcriptome profiles of recombinant Escherichia coli producing human insulin-like growth factor I fusion protein (IGF-If) during the high-cell-density fed-batch culture were analyzed using DNA microarrays. The expression levels of 529 genes were significantly altered after induction. About 200 genes were significantly down-regulated during the production of IGF-If after induction. Among these down-regulated genes, we rationally selected and coexpressed in E. coli producing IGF-If the prsA gene (encoding a phosphoribosyl pyrophosphate synthetase) and the glpF gene (encoding a glycerol transporter), which are involved in an early key step in the biosynthetic pathway of nucleotides and amino acids (Trp and His) and the first step in glycerol utilization, respectively. As a result, the production of IGF-If could be increased from 1.8 ± 0.13 (± standard deviation) to 4.3 ± 0.24 g/liter. The volumetric productivity was also increased from 0.36 ± 0.027 to 0.82 ± 0.048 g/liter/h. These results demonstrate that transcriptome profiling can provide invaluable information in designing engineered strains showing enhanced performance.

scholarly journals Expression of a synthetic gene encoding human insulin-like growth factor I in cultured mouse fibroblasts.

1987 ◽  
Vol 84 (9) ◽  
pp. 2638-2642 ◽  
Author(s):  
M. L. Bayne ◽  
M. A. Cascieri ◽  
B. Kelder ◽  
J. Applebaum ◽  
G. Chicchi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Human Insulin ◽  
Synthetic Gene ◽  
Insulin Like Growth Factor ◽  
Gene Encoding ◽  
Mouse Fibroblasts ◽  
Factor I ◽  
Growth Factor I
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