Localization of the human T-cell receptor gamma locus (TCRG) to 7p14→p15 by in situ hybridization

1991 ◽  
Vol 56 (1) ◽  
pp. 31-32 ◽  
Author(s):  
M. Bensmana ◽  
M.G. Mattei ◽  
M.-P. Lefranc
Keyword(s):  
In Situ Hybridization ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Human T Cell ◽  
Gamma Locus

scholarly journals An STS in the human T cell receptor gamma locus (located at 7p14–15)

1990 ◽  
Vol 18 (20) ◽  
pp. 6170-6170
Author(s):  
F. Bernard ◽  
P. Chuchana ◽  
G. Lefranc ◽  
M.-P. Lefranc
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Human T Cell ◽  
Gamma Locus

scholarly journals T cell receptor alpha chain genes are located on chromosome 14 at 14q11-14q12 in humans.

1985 ◽  
Vol 161 (5) ◽  
pp. 1255-1260 ◽  
Author(s):  
N Caccia ◽  
G A Bruns ◽  
I R Kirsch ◽  
G F Hollis ◽  
V Bertness ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Chromosome 14 ◽  
Receptor Alpha ◽  
Alpha Chain ◽  
Human T Cell ◽  
Receptor Alpha Chain ◽  
Cell Receptor Alpha

A cDNA clone encoding the alpha chain of the human T cell receptor was used in connection with somatic cell human-rodent hybrids to determine that the genes coding for the alpha chain are located on chromosome 14 in humans. In situ hybridization confirms this result and further localizes these genes to 14q11-14q12 on this chromosome. Since this region of chromosome has been shown to be nonrandomly involved in a number of T cell neoplasias, this assignment raises a number of interesting questions as to the possible involvement of the T cell receptor alpha chain genes in tumorigenesis.

scholarly journals A distinct wave of human T cell receptor gamma/delta lymphocytes in the early fetal thymus: evidence for controlled gene rearrangement and cytokine production.

1990 ◽  
Vol 172 (3) ◽  
pp. 847-859 ◽  
Author(s):  
M S Krangel ◽  
H Yssel ◽  
C Brocklehurst ◽  
H Spits
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cytokine Production ◽  
Cell Receptor ◽  
Gamma Delta ◽  
Fetal Thymus ◽  
Human T Cell ◽  
Delta Gene ◽  
Gamma Locus ◽  
Junctional Diversity

The rearrangement and expression of human T cell receptor (TCR)-gamma and -delta gene segments in clonal and polyclonal populations of early fetal and postnatal human TCR-gamma/delta thymocytes were examined. The data suggest that the TCR-gamma and -delta loci rearrange in an ordered and coordinated fashion. Initial rearrangements at the TCR-delta locus join V delta 2 to D delta 3, and initial rearrangements at the TCR-gamma locus join downstream V gamma gene segments (V gamma 1.8 and V gamma 2) to upstream J gamma gene segments associated with C gamma 1. These rearrangements are characterized by minimal junctional diversity. At later times there is a switch at the TCR-delta locus such that V delta 1 is joined to upstream D delta gene segments, and a switch at the TCR-gamma locus such that upstream V gamma gene segments are joined to downstream J gamma gene segments associated with C gamma 2. These rearrangements are characterized by extensive junctional diversity. Programmed rearrangement explains in part the origin of discrete subpopulations of peripheral blood TCR-gamma/delta lymphocytes that have been defined in previous studies. In addition, cytokine production by early fetal and postnatal TCR-gamma/delta thymocyte clones was examined. Fetal thymocyte clones produced significant levels of IL-4 and IL-5 following stimulation, whereas postnatal thymocyte clones did not produce these cytokines. Thus, these cell populations may represent functionally distinct subsets as well.

scholarly journals Early genetic events in T cell development analyzed by in situ hybridization.

1987 ◽  
Vol 165 (6) ◽  
pp. 1624-1638 ◽  
Author(s):  
D M Pardoll ◽  
B J Fowlkes ◽  
R I Lechler ◽  
R N Germain ◽  
R H Schwartz
Keyword(s):  
In Situ Hybridization ◽  
T Cell ◽  
T Cell Receptor ◽  
T Cell Development ◽  
Gene Activation ◽  
Cell Receptor ◽  
Fetal Thymus ◽  
Alpha Beta ◽  
Genetic Events

In situ hybridization was used to investigate the expression of T cell receptor (TCR) alpha, beta, and gamma mRNAs in developing fetal and adult precursor thymocytes. gamma transcription was observed at the earliest time tested (day 12), followed by beta 12 h later, and TCR alpha on day 16. The early beta transcripts appeared to be from unrearranged or incompletely rearranged (D-J-C) beta loci. V beta region transcription was first detectable on day 14 and transcription of different V beta genes was induced at different times. These results delineate a schedule sequence of TCR gene activation, which begins within 1 d after entry of stem cells into the fetal thymus.

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