Mapping of the creatine kinase M gene to 19q11→q12 in the rabbit genome

1989 ◽  
Vol 50 (2-3) ◽  
pp. 165-167 ◽  
Author(s):  
P.A. Martin-DeLeon ◽  
J. McLaughlin ◽  
E. Mitzel ◽  
S. Tailor
Keyword(s):  
Creatine Kinase ◽  
M Gene ◽  
Rabbit Genome

scholarly journals Creatine Kinase Gene Mutation in a Patient with Muscle Creatine Kinase Deficiency

2001 ◽  
Vol 47 (11) ◽  
pp. 1967-1973 ◽  
Author(s):  
Hiroshi Yamamichi ◽  
Shinpei Kasakura ◽  
Shunzi Yamamori ◽  
Ryu Iwasaki ◽  
Takumi Jikimoto ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Point Mutation ◽  
Tissue Sample ◽  
Direct Sequencing ◽  
Control Sample ◽  
M Protein ◽  
Myocardial Tissue ◽  
Polymorphism Analysis ◽  
M Gene ◽  
Exon 2

Abstract Background: We describe a 56-year-old woman admitted to the hospital with a diagnosis of acute myocardial infarction without an increase of serum creatine kinase (CK) activity during her clinical course. She died on the 11th hospital day, and the diagnosis was confirmed by autopsy. The patient had had no previous muscular symptoms. Methods: Expression of the CK-muscle (CK-M) protein in cardiac tissue was examined by immunoblotting and immunochemical staining. CK-M mRNA expression was estimated by semiquantitative reverse transcription–PCR. Gene structure of CK-M was determined by Southern blotting and direct sequencing of 2251 bp. Existence of a point mutation in the CK-M gene was examined by restriction fragment length polymorphism analysis of PCR products (PCR-RFLP) in the patient and in 108 controls. Results: CK-M protein in the myocardial tissue of the patient was substantially lower (103 ± 7 ng/mg protein) than in control myocardial tissue (35 800 ± 2860 ng/mg protein). Immunoreactive CK-M in the patient tissue sample was 0.3% of the value for the control sample. CK-M mRNA was 53-fold less in the patient sample compared with the control. This very low expression of CK-M mRNA was considered to be the primary reason for CK-M deficiency. Direct sequencing revealed a point mutation at residue 54 in exon 2, which was specific for the patient. No other abnormalities were found in the CK-M gene of the patient. Conclusions: This report identifies a molecular abnormality in human CK deficiency and discusses the physiologic relevance of CK-M.

99mTc-Aminohexylidendiphosphonate and 99mTc-Pyrophos phate in the Scintigraphic Diagnosis of Experimental Cardiomyopathy in Dogs

1987 ◽  
Vol 26 (05) ◽  
pp. 220-223 ◽  
Author(s):  
L. Hadaš ◽  
J. VižĎa ◽  
P. Kafka ◽  
Y. Mazurová ◽  
V. Palicka ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Plasma Levels ◽  
Maximum Accumulation ◽  
Kinase A ◽  
Good Agreement

Experimental cardiomyopathy was provoked in 24 dogs with high intravenous doses of adrenaline and theophylline. These lesions were studied by means of the new agent 99mTc-AHDP and 99mTc-PYP in comparison. Cardiomyopathy could be imaged as early as 4 h after the onset of involvement but not later than 7 days. A maximum accumulation occurred in lesions 24 h old. 99mTc uptake in the myocardium was graded scintigraphically. 99mTc-AHDP was accumulated in the altered’myocardium to a greater extent than 99mTc-PYP. Scintigraphic findings were in good agreement with plasma levels of creatine- kinase. A comparison with histology demonstrated that the maximum accumulation of radiopharmaceuticals occurred at the time when the development of myocardium involvement reached the stage of myocytolysis.

Molecular Characterisation of ESBL producer E. coli

2018 ◽  
Vol 1 (2) ◽  
pp. 40-57
Author(s):  
Abdulghani Alsamarai ◽  
Shler Khorshed ◽  
Imad Weli
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Female Student ◽  
Clinical Problem ◽  
Clinical Isolates ◽  
Molecular Characterisation ◽  
M Gene ◽  
Esbl Producer ◽  
E Coli

Background: Antibiotic resistance emerged as clinical problem challenge the effective treatment of infections. Virulence factor may play an important role in the influence of antimicrobial resistance. Objective: To determine the frequency of resistance gene in E. coli clinical isolates from women with urinary tract infection. Materials and Methods: Fifteen E.coli clinical isolates were tested by PCR to determine their molecular characterization. Results: The bla CTX –M gene was not detected in 6.7% out of the tested 15 E. coli clinical isolates from women with urinary tract infection. However, bla OXA gene was detected in all E. coli tested clinical isolates from pregnant women, female student and diabetic women with urinary tract infection. While bla TEM gene and bla SHV gene were not detected in 33.3% and 40% out of the tested E. coli clinical isolates respectively. Conclusions: Four types of ESBL genes were detected, and shows new trend of distribution, which indicated the predominance of OXA and CTX-M genes.

Human creatine kinase: Isoenzymes and logistics of energy distribution

1984 ◽  
Vol 44 (7) ◽  
pp. 611-615 ◽  
Author(s):  
Christer Sylvén ◽  
Eva Jansson ◽  
Anders Kallner ◽  
Kim Böök
Keyword(s):  
Creatine Kinase ◽  
Energy Distribution ◽  
Creatine Kinase Isoenzymes
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