Regional mapping of unique DNA sequences from human chromosome 3 derived from a flow-sorted chromosome library

1988 ◽  
Vol 48 (3) ◽  
pp. 156-159 ◽  
Author(s):  
L.M. Atchison ◽  
S.L. Naylor ◽  
J.J. Freed ◽  
R.L. Comis
Keyword(s):  
Human Chromosome ◽  
Dna Sequences ◽  
Chromosome 3 ◽  
Regional Mapping ◽  
Human Chromosome 3

Sau3A in situ digestion of human chromosome 3 pericentromeric heterochromatin. I. Differential digestion of α-satellite and satellite 1 DNA sequences

Genome ◽  
2001 ◽  
Vol 44 (1) ◽  
pp. 120-127 ◽  
Author(s):  
I. Buño ◽  
J.L. Fernández ◽  
C. López-Fernández ◽  
J.L. Díez-Martín ◽  
J. Gosálvez
Keyword(s):  
Human Chromosome ◽  
Dna Sequences ◽  
Pericentromeric Heterochromatin ◽  
Chromosome 3 ◽  
Human Chromosome 3 ◽  
Differential Digestion

Sau3A in situ digestion of human chromosome 3 pericentromeric heterochromatin. I. Differential digestion of α-satellite and satellite 1 DNA sequences

Genome ◽  
2001 ◽  
Vol 44 (1) ◽  
pp. 120-127 ◽  
Author(s):  
I Buño ◽  
J L Fernández ◽  
C López-Fernández ◽  
J L Díez-Martín ◽  
J Gosálvez
Keyword(s):  
Human Chromosome ◽  
Dna Sequences ◽  
Satellite Dna ◽  
Domain Size ◽  
Pericentromeric Heterochromatin ◽  
Chromosome 3 ◽  
Human Chromosome 3 ◽  
Domain 3 ◽  
Differential Digestion

In situ digestion with the restriction endonuclease (RE) Sau3A (Sau3A REISD) uncovers a polymorphism for the pericentromeric heterochromatin of human chromosome 3, which can be positively stained (3+) or not (3–), and has proven useful to differentiate donor and recipient cells after sex-matched bone marrow transplantation and to analyze the so-called hemopoietic chimerism. The aim of the present investigation was to obtain insight into the molecular basis of such polymorphism to optimize its use for chimerism quantification using methodological approaches other than REISD. To this end, fluorescence in situ hybridization (FISH) assays using probes for the satellite DNA sequences that mainly constitute chromosome 3 pericentromeric heterochromatin (α-satellite and satellite 1 DNA) were performed on control and Sau3A-digested chromosomes. The results obtained suggest that chromosome 3 α-satellite DNA is digested in all individuals studied, irrespective of the karyotype obtained by Sau3A REISD (3++, 3+–, 3--), and thus it does not seem to be involved in the polymorphism uncovered by Sau3A on this chromosome. Satellite 1 DNA is not digested in any case, and shows a polymorphism for its domain size, which correlates with the polymorphism uncovered by Sau3A in such a way that 3+ chromosomes show a large domain (3L) and 3– chromosomes show a small domain (3S). It seems, therefore, that the cause of the polymorphism uncovered by Sau3A on the pericentromeric region of chromosome 3 is a difference in the size of the satellite 1 DNA domain. Small satellite 1 DNA domains fall under the resolution level of REISD technique and are identified as 3–.Key words: heterochromatin, α-satellite DNA, classical satellite DNA, satellite 1 DNA, restriction endonucleases, FISH.

Precise Localization of Aphidicolin-Induced Breakpoints on the Short Arm of Human Chromosome 3

Genomics ◽  
1995 ◽  
Vol 27 (2) ◽  
pp. 358-361 ◽  
Author(s):  
William Paradee ◽  
Chadwick Mullins ◽  
Zhanquan He ◽  
Thomas Glover ◽  
Charles Wilke ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Chromosome 3 ◽  
Precise Localization ◽  
Human Chromosome 3

Physical and genetic mapping of human chromosome 3 loci containing microsatellite repeats

1994 ◽  
Vol 2 (6) ◽  
pp. 423-427 ◽  
Author(s):  
Steve Gerken ◽  
Ethan Whisenant ◽  
Terry Varkony ◽  
Sean Todd ◽  
Robert Gemmill ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Genetic Mapping ◽  
Chromosome 3 ◽  
Microsatellite Repeats ◽  
Human Chromosome 3

Sperm typing allows accurate measurement of the recombination fraction between D3S2 and D3S3 on the short arm of human chromosome 3

Genomics ◽  
1992 ◽  
Vol 12 (4) ◽  
pp. 683-687 ◽  
Author(s):  
Rene Hubert ◽  
Vincent P. Stanton ◽  
Hiroyuki Aburatani ◽  
John Warren ◽  
Honghua Li ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Recombination Fraction ◽  
Chromosome 3 ◽  
Human Chromosome 3 ◽  
Sperm Typing

scholarly journals Not I linking/jumping clones of human chromosome 3: mapping of the TFRC, RAB7 and HAUSP genes to regions rearranged in leukemia and deleted in solid tumors

FEBS Letters ◽  
1997 ◽  
Vol 419 (2-3) ◽  
pp. 181-185 ◽  
Author(s):  
V.I Kashuba ◽  
R.Z Gizatullin ◽  
A.I Protopopov ◽  
R Allikmets ◽  
S Korolev ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Solid Tumors ◽  
Chromosome 3 ◽  
Human Chromosome 3

scholarly journals Restoration of the Cellular Senescence Program and Repression of Telomerase by Human Chromosome 3

1995 ◽  
Vol 86 (10) ◽  
pp. 899-904 ◽  
Author(s):  
Hiroshi Ohmura ◽  
Hidetoshi Tahara ◽  
Mikio Suzuki ◽  
Toshinori Ide ◽  
Motoyuki Shimizu ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Cellular Senescence ◽  
Chromosome 3 ◽  
Human Chromosome 3 ◽  
Senescence Program

Seven genes on the short arm of human chromosome 3 map to two regions on Macropus eugenii (tammar wallaby) chromosome 2

1994 ◽  
Vol 65 (4) ◽  
pp. 228-232 ◽  
Author(s):  
D.A. Miller ◽  
O.J. Miller ◽  
D. Francis ◽  
P.F.R. Little ◽  
J.A.M. Graves
Keyword(s):  
Human Chromosome ◽  
Tammar Wallaby ◽  
Chromosome 3 ◽  
Chromosome 2 ◽  
Macropus Eugenii ◽  
Human Chromosome 3
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