Studies on isolated cloned populations from irradiated human embryonic cell cultures

1979 ◽  
Vol 24 (3) ◽  
pp. 150-159 ◽  
Author(s):  
C.L.Y. Lee ◽  
S.H.S. Lee ◽  
O.P. Kamra
Keyword(s):  
Cell Cultures ◽  
Embryonic Cell ◽  
Human Embryonic Cell

Cytomegalovirus Type Cytopathic Changes in Spontaneously Degenerating Human Embryonic Cell Cultures

Nature ◽  
1967 ◽  
Vol 214 (5086) ◽  
pp. 419-420 ◽  
Author(s):  
PETER DIOSI ◽  
LIVIA BABUSCEAC ◽  
CAMELIA DAVID
Keyword(s):  
Cell Cultures ◽  
Embryonic Cell ◽  
Human Embryonic Cell

scholarly journals Analysis on the prokaryotic microbiome in females and embryonic cell cultures of Rhipicephalus sanguineus tropical and temperate lineages from two specific localities in Brazil

2021 ◽  
Vol 30 (3) ◽  
Author(s):  
Mayara de Cassia Luzzi ◽  
Lucas Amoroso Lopes de Carvalho ◽  
Daniel Guariz Pinheiro ◽  
Leidiane Lima-Duarte ◽  
Jaqueline Valéria Camargo ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Rhipicephalus Sanguineus ◽  
Distribution Patterns ◽  
Embryonic Cell ◽  
Tick Cell ◽  
Illumina Platform ◽  
Region Gene ◽  
Host Environment ◽  
Canine Monocytic Ehrlichiosis ◽  
Vertebrate Hosts

Abstract Two lineages of Rhipicephalus sanguineus are known in Brazil: the temperate or southern and the tropical or northern populations. The distribution patterns of both lineages of R. sanguineus have epidemiological implications that can affect vectorial competence concerning Ehrlichia canis, the agent of canine monocytic ehrlichiosis. Intending to identify the microbiomes of both lineages and compare microorganisms in R. sanguineus, we used the 16S rRNA (V4-V5 region) gene-based metataxonomic approach, through NGS sequencing on the MiSeq Illumina platform. We selected specimens of females from the environment and samples of primary embryonic cell cultures, from both lineages, and this was the first study to investigate the prokaryotic microbiome in tick cell cultures. The results showed that many bacterial taxa detected in the samples were typical members of the host environment. A significant diversity of microorganisms in R. sanguineus females and in embryonic cell cultures from both lineages was found, with emphasis on the presence of Coxiella in all samples, albeit in different proportions. The Coxiella species present in the two lineages of ticks may be different and may have co-evolved with them, thus driving different patterns of interactions between ticks and the pathogens that they can harbor or transmit to vertebrate hosts.

Human embryonic cell growth assay for teratogens with or without metabolic activation system using microplate

1988 ◽  
Vol 8 (5) ◽  
pp. 265-272 ◽  
Author(s):  
Toshie Tsuchiya ◽  
Atsuko Matuoka ◽  
Setsuko Sekita ◽  
Takuzo Hisano ◽  
Atsushi Takahashi ◽  
...  
Keyword(s):  
Cell Growth ◽  
Metabolic Activation ◽  
Embryonic Cell ◽  
Activation System ◽  
Growth Assay ◽  
Human Embryonic Cell

scholarly journals Initiation of primary cell cultures from embryonic Haemaphysalis bispinosa ticks 

2017 ◽  
Vol 22 (3) ◽  
pp. 323 ◽  
Author(s):  
Fang-shiang Lim ◽  
Jing-jing Khoo ◽  
Fezshin Chen ◽  
Lesley Bell-sakyi ◽  
Chee-sieng Khor ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Embryonic Cell ◽  
Rrna Gene ◽  
Hard Tick ◽  
Tick Cell ◽  
Haemaphysalis Bispinosa ◽  
Ornithodoros Moubata ◽  
Floating Cell ◽  
Axenic Conditions

Tick cell cultures have been widely used as an important tool for the study of tick-associated microorganisms, specifically for medically important bacteria or viruses that may be difficult to isolate or culture in axenic conditions. In this study, primary embryonic tick cell cultures were initiated separately from each of the egg batches laid by 10 female ticks belonging to the hard tick genus Haemaphysalis. All cultures were maintained at 28°C. After 10 months, 4 healthy cultures were identified with the potential for developing into continuous tick cell lines. These cultures comprise large cells predominantly forming floating cell clumps with multicellular vesicles, which are morphologically similar to cell lines derived from the soft tick Ornithodoros moubata. Subculture has not yet been performed due to the low cell density at the time of writing. Amplification and sequencing of a fragment of the 16S rRNA gene from DNA extracted from the parent ticks showed 99%-100% similarity to published sequences of Haemaphysalis bispinosa. This is the first report of the initiation of embryonic cell cultures from Haemaphysalis ticks found in Malaysia. Such tick cell cultures will be useful for studies of tick-borne pathogens in this region, where recent studies have shown that Haemaphysalis ticks are highly represented and harbor medically important bacteria. 

scholarly journals Effects of Glycerol on Chicken Spermatozoa Incubated In Vitro at 41 C in Oviducal and Embryonic Cell Cultures

1988 ◽  
Vol 67 (1) ◽  
pp. 135-140 ◽  
Author(s):  
J.R. LATORRE ◽  
G.C. HARRIS ◽  
J.K. SKEELES ◽  
Z.B. JOHNSON
Keyword(s):  
Cell Cultures ◽  
Embryonic Cell
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