Ellobius lutescens, Th. (Rodentia, Microtinae): Q-, R-, and replication banding, patterns

1979 ◽  
Vol 23 (1-2) ◽  
pp. 117-123 ◽  
Author(s):  
M. Wolf ◽  
W. Schempp ◽  
W. Vogel
Keyword(s):  
Banding Patterns ◽  
Replication Banding ◽  
Ellobius Lutescens

scholarly journals Chromosome Banding in Amphibia. XXXII. The Genus Xenopus (Anura, Pipidae)

2015 ◽  
Vol 145 (3-4) ◽  
pp. 201-217 ◽  
Author(s):  
Michael Schmid ◽  
Claus Steinlein
Keyword(s):  
Chromosome Pair ◽  
Repetitive Sequences ◽  
5S Rdna ◽  
Mitotic Chromosomes ◽  
Banding Patterns ◽  
Single Chromosome ◽  
Replication Banding ◽  
Rdna Sequences ◽  
High Degree ◽  
Replication Bands

Mitotic chromosomes of 16 species of the frog genus Xenopus were prepared from kidney and lung cell cultures. In the chromosomes of 7 species, high-resolution replication banding patterns could be induced by treating the cultures with 5-bromodeoxyuridine (BrdU) and deoxythymidine (dT) in succession, and in 6 of these species the BrdU/dT-banded chromosomes could be arranged into karyotypes. In the 3 species of the clade with 2n = 20 and 4n = 40 chromosomes (X. tropicalis, X. epitropicalis, X. new tetraploid 1), as well as in the 3 species with 4n = 36 chromosomes (X. laevis, X. borealis, X. muelleri), the BrdU/dT-banded karyotypes show a high degree of homoeology, though differences were detected between these groups. Translocations, inversions, insertions or sex-specific replication bands were not observed. Minor replication asynchronies found between chromosomes probably involve heterochromatic regions. BrdU/dT replication banding of Xenopus chromosomes provides the landmarks necessary for the exact physical mapping of genes and repetitive sequences. FISH with an X. laevis 5S rDNA probe detected multiple hybridization sites at or near the long-arm telomeric regions in most chromosomes of X. laevis and X. borealis, whereas in X. muelleri, the 5S rDNA sequences are located exclusively at the long-arm telomeres of a single chromosome pair. Staining with the AT base pair-specific fluorochrome quinacrine mustard revealed brightly fluorescing heterochromatic regions in the majority of X. borealis chromosomes which are absent in other Xenopus species.

scholarly journals Use of lymphocyte cultures for BrdU replication banding patterns in anuran species (Amphibia)

1998 ◽  
Vol 21 (4) ◽  
pp. 471-476 ◽  
Author(s):  
Sanae Kasahara ◽  
Ana Paula Zampieri Silva ◽  
Simone Lilian Gruber
Keyword(s):  
Lymphocyte Culture ◽  
Banding Patterns ◽  
Replication Banding ◽  
Anuran Species ◽  
Lymphocyte Cultures

We describe the standardization of lymphocyte culture procedures in order to improve cytological preparations of anuran species. This methodology permits the use of 5-bromodeoxyuridine (BrdU) treatment to obtain replication banding patterns in the chromosomes of these species.

scholarly journals R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)

1999 ◽  
Vol 22 (2) ◽  
pp. 201-204 ◽  
Author(s):  
Edson Luis Maistro ◽  
Fausto Foresti ◽  
Claudio Oliveira
Keyword(s):  
Banding Pattern ◽  
Banding Technique ◽  
Fish Genome ◽  
Banding Patterns ◽  
Technical Problems ◽  
Replication Banding ◽  
Astyanax Scabripinnis ◽  
Fish Chromosomes ◽  
Restriction Endonuclease Bamhi

The absence of longitudinal bands in fish chromosomes has been associated with technical problems in chromosome preparations or the absence of a structural compartmentalization in the fish genome. In the present study, a R-banding pattern was obtained using a replication banding technique by in vivo treatment with 5-bromodeoxyuridine (5-BrdU). G-banding patterns were obtained after trypsin treatment and also after chromosome cleavage by in situ treatment with the restriction endonuclease BamHI. A similar G-banding pattern was also obtained after cleavage with the endonuclease HinfI. Presence of a resolute R- and G-banding patterns shows that Astyanax scabripinnis paranae chromosomes could present an isochore-like structure similar to that found in other vertebrates.

High catabolism of BrdU may explain unusual sister chromatid differentiation and replication banding patterns in cancer cells

1991 ◽  
Vol 53 (1) ◽  
pp. 23-34 ◽  
Author(s):  
Liliane Massaad ◽  
Anne-Marie Venuat ◽  
Catherine Luccioni ◽  
Jacqueline Beaumatin ◽  
Nicole Lemieux ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Sister Chromatid ◽  
Banding Patterns ◽  
Replication Banding ◽  
Sister Chromatid Differentiation

Replication banding patterns in Atlantic salmon (Salmo salar)

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 440-444 ◽  
Author(s):  
A. M. Pendás ◽  
P. Morán ◽  
E. García-Vázquez
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Banding Pattern ◽  
Staining Method ◽  
Chromosome Size ◽  
Banding Patterns ◽  
Replication Banding ◽  
C Banding ◽  
Atlantic Salmon Salmo Salar

Replication banding patterns have been obtained from in vivo treatment of Salmo salar using a modification of the 5-BrdU technique and in kidney cultures using the FPG staining method. Most of the chromosome pairs were identified in the karyotype based on the banding pattern, chromosome size, and centromere position. C-banding and replication banding patterns were compared.Key words: karyotype, replication banding, Salmo salar.

scholarly journals Characterization of the late replication banding patterns in barley chromosomes.

1997 ◽  
Vol 72 (3) ◽  
pp. 125-130 ◽  
Author(s):  
Sakurako Uozu ◽  
Tetsuya Nakazaki ◽  
Takatoshi Tanisaka
Keyword(s):  
Late Replication ◽  
Banding Patterns ◽  
Replication Banding
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