Electrophoretic pattern of hypoxanthine phosphoribosyltransferase produced in Chinese hamster cells following incorporation of human chromosomes

1975 ◽  
Vol 14 (3-6) ◽  
pp. 462-463 ◽  
Author(s):  
G.J. Wullems ◽  
J. van der Horst
Keyword(s):  
Chinese Hamster ◽  
Electrophoretic Pattern ◽  
Hypoxanthine Phosphoribosyltransferase ◽  
Human Chromosomes ◽  
Chinese Hamster Cells

scholarly journals Gene Mapping in Marsupials and Monotremes, V. Synteny between Hypoxanthine Phosphoribosyltransferase and Phosphoglycerate Kinase in the Platypus

1988 ◽  
Vol 41 (2) ◽  
pp. 231 ◽  
Author(s):  
Jaclyn M Watson ◽  
Jennifer A MarshalI Graves
Keyword(s):  
X Chromosome ◽  
Chromosomal Location ◽  
Chinese Hamster ◽  
Low Frequency ◽  
Phosphoglycerate Kinase ◽  
Chromosomal Assignment ◽  
Hypoxanthine Phosphoribosyltransferase ◽  
Syntenic Group ◽  
Chinese Hamster Cells ◽  
Synteny Group

In order to extend comparative mapping studies to the monotreme mammals (subclass Prototheria), somaticcell hybrids were obtained between Chinese-hamster cells deficient in hypoxanthine phosphoribosyltransferase (HPRT) and platypus fibroblasts. The characteristics of these hybrids closely resemble those of metatherian x eutherian hybrids, in that they are recovered at low frequency and they rapidly segregate and fragment platypus chromosomes. Biochemical and cytological studies of the hybrids, their subclones and HPRT-deficient revertants indicate that phosphoglycerate kinase is syntenic with HPRT in the platypus (as it is in other mammals); however, the studies do not permit chromosomal assignment of the syntenic group. The implications of the chromosomal location of this ancient synteny group for the evolution of the mammalian X chromosome are discussed.

Sign in / Sign up

Export Citation Format

Share Document