Rapid processing of primary embryonic tissues for chromosome banding pattern analysis

1972 ◽  
Vol 11 (5) ◽  
pp. 424-435 ◽  
Author(s):  
H.P. Klinger
Hereditas ◽  
2009 ◽  
Vol 76 (2) ◽  
pp. 305-314 ◽  
Author(s):  
LIISA HALKKA ◽  
O. HALKKA ◽  
U. SKARÉN ◽  
VERONICA SÖDERLUND

1974 ◽  
Vol 13 (1-2) ◽  
pp. 117-122 ◽  
Author(s):  
C.-C. Lin

Gerontology ◽  
1978 ◽  
Vol 24 (5) ◽  
pp. 369-372 ◽  
Author(s):  
A. Brun ◽  
L. Gustafson ◽  
F. Mitelman

2021 ◽  
Author(s):  
Sanjay Kumar ◽  
Asikho Kiso ◽  
N. Abenthung Kithan

Chromosome identification depends on the morphological features of the chromosome and therefore karyotype and its banding pattern analyses are the most suitable technique to identify each and every chromosome of a chromosome complement. Moreover, aberrations caused by breaks play an important role in the evolution of a chromosome set and chromosome complement by decreasing or increasing the chromosome number. Therefore, both the aspects are discussed in detail in the present chapter. At present, the chapter will highlight the karyotype and its components, karyotype trends, evolution and its role in speciation, banding pattern and techniques, chromosome differentiation and linearization, banding applications and their uses, detection and analysis of chromosomal aberrations, chromosome and chromatid types of aberrations and mechanism of the formation of chromosome aberrations and breaks for karyotype evolutionary trends.


1991 ◽  
Vol 11 (9) ◽  
pp. 4572-4580
Author(s):  
C W Greider

Telomerase synthesizes tandem repeats of the sequence d(TTGGGG) onto input d(TTGGGG)n primer oligonucleotides (C. W. Greider and E. H. Blackburn, Cell 43:405-413). An intrinsic RNA component of the enzyme provides the template for d(TTGGGG)n repeat synthesis [C. W. Greider and E. H. Blackburn, Nature (London) 337:331-337, 1989; G.-L. Lu, J. D. Bradley, L. D. Attardi, and E. H. Blackburn, Nature (London) 344:126-132, 1990]. In a typical reaction, products greater than 2,000 nucleotides were synthesized in 60 min. Dilution and primer challenge experiments showed that these long products were synthesized processively. The apparent processivity was not due to a higher affinity of the enzyme for long d(TTGGGG) products over the shorter competitors. The degree of processivity was quantitated; telomerase synthesized approximately 520 nucleotides before half of the enzyme had dissociated. After dissociating, telomerase reinitiated d(TTGGGG)n synthesis on new primer oligonucleotides. The products from a telomerase reaction have a characteristic 6-nucleotide banding pattern (C. W. Greider and E. H. Blackburn, Cell 51:887-898, 1987). A strong pause in the reaction occurs after the addition of the first G in the sequence d(TTGGGG). Both the processivity and the banding pattern analysis imply that in the elongation mechanism there must be a translocation step after the 9 nucleotides of internal template RNA have been copied to the extreme 5' end.


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