Comparison of banding patterns of human chromosomes obtained with heating, fluorescence, and proteolytic digestion

1972 ◽  
Vol 11 (2) ◽  
pp. 113-116 ◽  
Author(s):  
B. Dutrillaux ◽  
Catherine Finaz ◽  
J. De Grouchy ◽  
J. Lejeune
Keyword(s):  
Human Chromosomes ◽  
Proteolytic Digestion ◽  
Banding Patterns

QUINACRINE FLUORESCENCE AND Q-BANDING PATTERNS OF HUMAN CHROMOSOMES.: I. Effects of Varying Factors

1975 ◽  
Vol 17 (1) ◽  
pp. 81-92 ◽  
Author(s):  
C. C. Lin ◽  
H. van de Sande ◽  
W. K. Smink ◽  
D. R. Newton
Keyword(s):  
Exposure Time ◽  
Uv Light ◽  
Human Chromosomes ◽  
Concave Mirror ◽  
Banding Patterns ◽  
Fluorescent Microscope ◽  
Sperm Dna ◽  
Quinacrine Fluorescence ◽  
Well Differentiated ◽  
Salmon Sperm Dna

Various factors involved in the production of "Q-bands" have been studied. It was found that a Zeiss standard WL fluorescent microscope required a shorter exposure time for photography as compared to a Zeiss photomicroscope. The minimal exposure time was obtained when the standard WL microscope was equipped with a UV light source containing a DC powered mercury burner and a concave mirror. Further, the pH and type of water used in the staining, washing and mounting of the slide were also important factors in producing clear and well differentiated "Q-bands". It also appears that the factors involved in the production of "Q-bands" effect the enhancement or quenching of fluorescence by poly d(A-T).poly d(A-T) and salmon sperm DNA or poly dG∙poly dC respectively. This preliminary report also suggests that DNA or polynucleotides with a specific base sequence may play an important role in Q-banding patterns on chromosomes.

G-banding patterns of high-resolution human chromosomes 6?22, X, and Y

1979 ◽  
Vol 49 (3) ◽  
pp. 291-306 ◽  
Author(s):  
J. J. Yunis ◽  
D. W. Ball ◽  
J. R. Sawyer
Keyword(s):  
High Resolution ◽  
Human Chromosomes ◽  
Banding Patterns

The Effect of Mitomycin C on Human Chromosomes with G-banding Patterns

1976 ◽  
Vol 17 (2) ◽  
pp. 115
Author(s):  
Tae Sang Tchun ◽  
Sahsook Hahn ◽  
Dong Sik Kim
Keyword(s):  
Mitomycin C ◽  
Human Chromosomes ◽  
Banding Patterns

scholarly journals MICROFLUOROMETRIC ANALYSIS OF DEOXYRIBONUCLEIC ACID REPLICATION KINETICS AND SISTER CHROMATID EXCHANGES IN HUMAN CHROMOSOMES

1974 ◽  
Vol 22 (7) ◽  
pp. 478-491 ◽  
Author(s):  
SAMUEL A. LATT
Keyword(s):  
High Resolution ◽  
Dna Synthesis ◽  
Sister Chromatid ◽  
Deoxyribonucleic Acid ◽  
Sister Chromatid Exchanges ◽  
Human Chromosomes ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Human Leukocytes ◽  
Chromatid Exchanges

Fluorescence of the dye 33258 Hoechst, when bound to chromosomes, is partially quenched by the incorporation of 5-bromodeoxyuridine into chromosomal deoxyribonucleic acid (DNA). This effect allows microfluorometric analysis of DNA synthesis. Metaphase chromosomes from cultured human leukocytes which have incorporated 5-bromodeoxyuridine for a portion of the DNA synthesis period exhibit reduced 33258 Hoechst fluorescence in 5-bromodeoxyuridine-containing regions. Regions synthesizing DNA during a particular interval can thus be highlighted by the appropriate protocol of 5-bromodeoxyuridine administration. Chromosomes from cells which have replicated twice in medium containing 5-bromodeoxyuridine exhibit one brightly and one dully fluorescing chromatid, reflecting incorporation of 5-bromodeoxyuridine into one or two chains of chromatid DNA, respectively. Sister chromatid exchanges, evident as sharply demarcated reciprocal alterations in fluorescence along chromosomes, can be located relative to quinacrine banding patterns. This fluorometric approach should be useful in many instances as a convenient, high resolution alternative to autoradiography.

Banding patterns in human chromosomes: production by proteolytic enzymes

1972 ◽  
Vol 63 (5) ◽  
pp. 296-297 ◽  
Author(s):  
C. L. Y. LEE ◽  
J. P. WELCH ◽  
E. J. T. WINSOR
Keyword(s):  
Proteolytic Enzymes ◽  
Human Chromosomes ◽  
Banding Patterns

scholarly journals Specific Banding Patterns of Human Chromosomes

1971 ◽  
Vol 68 (9) ◽  
pp. 2073-2077 ◽  
Author(s):  
M. E. Drets ◽  
M. W. Shaw
Keyword(s):  
Human Chromosomes ◽  
Banding Patterns
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