Estrogen-Receptor-Negative Breast Cancer Tissue Is Chemosensitive in vitro Compared with Estrogen-Receptor-Positive Tissue

1990 ◽  
Vol 22 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Y. Maehara ◽  
Y. Emi ◽  
Y. Sakaguchi ◽  
T. Kusumoto ◽  
Y. Kakeji ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Estrogen Receptor Positive ◽  
Estrogen Receptor Negative

Evaluation of photodynamic therapy efficiency using an in vitro three-dimensional microfluidic breast cancer tissue model

Lab on a Chip ◽  
2015 ◽  
Vol 15 (3) ◽  
pp. 735-744 ◽  
Author(s):  
Yamin Yang ◽  
Xiaochuan Yang ◽  
Jin Zou ◽  
Chao Jia ◽  
Yue Hu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Photodynamic Therapy ◽  
Three Dimensional ◽  
Therapeutic Agents ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Tissue Model

A microfluidic-based in vitro three-dimensional (3D) breast cancer tissue model was established for determining the efficiency of photodynamic therapy (PDT) with therapeutic agents (photosensitizer and gold nanoparticles) under various irradiation conditions.

Estrogen receptor (ER) in breast cancer tissue of premenopausal patients: are some ER− findings false due to down-regulation?

2005 ◽  
Vol 64 (5) ◽  
pp. 1069-1070
Author(s):  
Sven Kurbel ◽  
Damir Kovačić ◽  
Jozo Kristek ◽  
Vladimir Šišljagić ◽  
Ivan Mihaljević
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Down Regulation ◽  
Premenopausal Patients

scholarly journals Patient-derived tumor spheroid cultures as a promising tool to assist personalized therapeutic decisions in breast cancer

2021 ◽  
Author(s):  
Sarah U Hofmann ◽  
Raichel Cohen-Harazi ◽  
Yael Maizels ◽  
Igor Koman
Keyword(s):  
Breast Cancer ◽  
Treatment Plan ◽  
Statistical Significance ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Intrinsic Resistance ◽  
Significant Group ◽  
Chemotherapy Drugs ◽  
Therapeutic Decisions

Breast cancer is the most common cause of cancer related death in women. Treatment of breast cancer has many limitations including a lack of accurate biomarkers to predict success of chemotherapy and intrinsic resistance of a significant group of patients to the gold standard of therapy. Therefore, new tools are needed to provide doctors with guidance in choosing the most effective treatment plan for a particular patient and thus to increase the survival rate for breast cancer patients. Here, we present a successful method to grow in vitro spheroids from primary breast cancer tissue. Samples were received in accordance with relevant ethical guidelines and regulations. After tissue dissociation, in vitro spheroids were generated in a scaffold-free 96-well plate format. Spheroid composition was investigated by immunohistochemistry (IHC) of epithelial (Pan Cytokeratin (panCK)), stromal (Vimentin) and breast cancer-specific markers (ER, PR, HER2, GATA-3). Growth and cell viability of the spheroids were assessed upon treatment with multiple anti-cancer compounds. Students t-test and two-way ANOVA test were used to determine statistical significance. We were able to successfully grow spheroids from 27 out of 31 samples from surgical resections of breast cancer tissue from previously untreated patients. Recapitulation of the histopathology of the tissue of origin was confirmed. Furthermore, a drug panel of standard first- and second-line chemotherapy drugs used to treat breast cancer was applied to assess the viability of the patient-derived spheroids and revealed variation between samples in the response of the spheroids to different drug treatments. We investigated the feasibility and the utility of an in vitro patient-derived spheroid model for breast cancer therapy, and we conclude that spheroids serve as a highly effective platform to explore cancer therapeutics and personalized treatment efficacy. These results have significant implications for the application of this model in clinical personalized medicine.

scholarly journals Virtual screening of natural compounds as combinatorial agents from indian medicinal plants against estrogen positive breast cancer

2020 ◽  
Vol 3 (10) ◽  
pp. 266-275
Author(s):  
Shaleen Jain ◽  
Dr. Asmita Das
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Virtual Screening ◽  
Medicinal Plants ◽  
Natural Compounds ◽  
Binding Energies ◽  
Common Amino Acid ◽  
Estrogen Receptor Positive ◽  
Positive Breast Cancer

Facing worldwide challenges associated with multifactorial etiology of breast cancer, designing of combinatorial therapies using natural compounds is currently the emergent way of treating several cancers including breast cancer in a synergistic way, which may mitigate several problems associated with multiple receptor targeting. In this research, Estrogen receptor positive breast cancer was taken as prototype and several key receptors associated with this particular disease were targeted by virtual screening of natural compounds found in Indian originated medicinal plants using Computer aided Drug Designing (CADD) strategies. We found the combination of Carpusin, Paulownin Cornigerine, Nororientaline, Oryzalexin B, Romucosine H and Colchicine as effective against six potential receptors i.e. FGFR2, ESR1, PIK3CA, PIK3CB, PIK3CD and AR in Estrogen receptor positive breast cancer with their binding energies in the range of ∆G ≤ -8.0 Kcal/mol as well as significant number of common amino acid binding residues as compared with binding sites of receptors. Thus this research holds significant implications for the designing of combinatorial therapeutic agents against breast cancer which can be further tested in-vitro and in-vivo to prove their synergistic efficiency.

Sign in / Sign up

Export Citation Format

Share Document