Comparative Distribution of Vasopressin and Oxytocin Neurons in the Rat Brain Using a Double-Label Procedure

1986 ◽  
Vol 44 (2) ◽  
pp. 235-246 ◽  
Author(s):  
Anna Hou-Yu ◽  
Alfred T. Lamme ◽  
Earl A. Zimmerman ◽  
Ann-Judith Silverman
Keyword(s):  
Rat Brain ◽  
Label Procedure ◽  
Double Label

scholarly journals Colocalization of microtubule-associated protein 1A and microtubule-associated protein 2 on neuronal microtubules in situ revealed with double-label immunoelectron microscopy.

1987 ◽  
Vol 104 (6) ◽  
pp. 1575-1578 ◽  
Author(s):  
Y Shiomura ◽  
N Hirokawa
Keyword(s):  
Rat Brain ◽  
Immunoelectron Microscopy ◽  
Electron Microscopic ◽  
Gold Particles ◽  
Rabbit Polyclonal Antibody ◽  
Double Label ◽  
Cross Bridges ◽  
Secondary Antibodies

Microtubule-associated protein 1A (MAP1A) and microtubule-associated protein 2 (MAP2) were shown to be colocalized on the same microtubules (MTs) within neuronal cytoskeletons by double-label immunoelectron microscopy. To investigate the electron microscopic disposition of MAP1A and MAP2 and their relationship to MTs in vivo, and to determine whether there are different subsets of MTs which specifically bind either MAP1 or MAP2, we employed a double-label immunogold procedure on rat cerebella using mouse monoclonal antibody against rat brain MAP1A and affinity-purified rabbit polyclonal antibody against rat brain MAP2. MAP1A and MAP2 were identified with secondary antibodies coupled to 10- and 5-nm gold particles, respectively. In Purkinje cell dendrites, both 10- and 5-nm gold particles were observed to be studded on the fuzzy structures attached to the same MTs. Many such structures connected MTs to each other. There was no particular MT which bound either MAP1A or MAP2 alone. Furthermore, there seemed to be no specific regions on MTs where either MAP1A or MAP2 was specifically attached. Hence, we conclude that MAP1A and MAP2 are colocalized on MTs in dendrites and assume that MAP1A and MAP2 have some interrelationship in vivo and that their interactions are responsible for forming the network of cross-bridges between MTs and MTs in neuronal cytoskeletons.

scholarly journals Ultrastructural identification of noradrenergic nerve terminals and vasopressin-containing neurons of the paraventricular nucleus in the same thin section.

1983 ◽  
Vol 31 (9) ◽  
pp. 1151-1156 ◽  
Author(s):  
A J Silverman ◽  
A Hou-Yu ◽  
B J Oldfield
Keyword(s):  
Thin Section ◽  
Paraventricular Nucleus ◽  
Epoxy Resins ◽  
Ultrathin Section ◽  
Nerve Terminals ◽  
Label Procedure ◽  
Thin Sectioning ◽  
Cell Groups ◽  
Ultrastructural Radioautography ◽  
Double Label

Since many peptidergic cell groups receive a diverse and complex monoaminergic innervation, we have developed a double-label procedure to visualize a peptide and a catecholamine in the same ultrathin section. Radiolabeled norepinephrine (NE) is applied locally and its reuptake into NE terminals is demonstrated by ultrastructural radioautography. Controls in this and other studies demonstrate that the NE labels only NE (and possibly epinephrine) terminals and not dopaminergic or serotonergic terminals. In the same tissue, vasopressin is localized by immunocytochemistry on unembedded sections that are subsequently embedded in epoxy resins for thin sectioning. The procedure as described here shows that NE terminals in the periventricular zone of the paraventricular nucleus of the hypothalamus innervate both vasopressin-positive and vasopressin-negative structures. This technique is useful in determining the chemical connectivity of the hypothalamus.

cDNA cloning and expression of a gamma-aminobutyric acidA receptor epsilon-subunit in rat brain

2000 ◽  
Vol 12 (12) ◽  
pp. 4318-4330 ◽  
Author(s):  
Nathalie Moragues ◽  
Philippe Ciofi ◽  
Pierrette Lafon ◽  
Marie-Francoise Odessa ◽  
Gerard Tramu ◽  
...  
Keyword(s):  
Rat Brain ◽  
Cdna Cloning ◽  
Cloning And Expression ◽  
Epsilon Subunit

Antisense knockdown of the glial glutamate transporter GLT-1 exacerbates hippocampal neuronal damage following traumatic injury to rat brain

2001 ◽  
Vol 13 (1) ◽  
pp. 119-128 ◽  
Author(s):  
Vemuganti L. Raghavendra Rao ◽  
Aclan Dogan ◽  
Kellie K. Bowen ◽  
Kathryn G. Todd ◽  
Robert J. Dempsey
Keyword(s):  
Rat Brain ◽  
Neuronal Damage ◽  
Traumatic Injury ◽  
Glutamate Transporter ◽  
Glial Glutamate Transporter ◽  
Antisense Knockdown

Inhibition of Rat Brain Monoamine Oxidase Activities by Psoralen and Isopsoralen: Implications for the Treatment of Affective Disorders

2001 ◽  
Vol 88 (2) ◽  
pp. 75-80 ◽  
Author(s):  
Ling Dong Kong ◽  
Ren Xiang Tan ◽  
Anthony Yiu Ho Woo ◽  
Christopher Hon Ki Cheng2Note
Keyword(s):  
Monoamine Oxidase ◽  
Rat Brain ◽  
Affective Disorders ◽  
Brain Monoamine
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