Suppression of Survivin Expression by Short Hairpin RNA Induces Apoptosis in Human Laryngeal Carcinoma Cells

ORL ◽  
2008 ◽  
Vol 70 (3) ◽  
pp. 168-175 ◽  
Author(s):  
Xiu-mei Chen ◽  
Xin-yong Luan ◽  
Da-peng Lei ◽  
Xiao-jie Ma ◽  
Xue-xia Liu ◽  
...  
Keyword(s):  
Laryngeal Carcinoma ◽  
Survivin Expression ◽  
Short Hairpin Rna ◽  
Carcinoma Cells ◽  
Hairpin Rna ◽  
Short Hairpin ◽  
Human Laryngeal Carcinoma

scholarly journals Downregulation of MDC1 and 53BP1 by short hairpin RNA enhances radiosensitivity in laryngeal carcinoma cells

2015 ◽  
Vol 34 (1) ◽  
pp. 251-257 ◽  
Author(s):  
QIHENG GOU ◽  
YUXIN XIE ◽  
LEI LIU ◽  
KEQI XIE ◽  
YANXIA WU ◽  
...  
Keyword(s):  
Laryngeal Carcinoma ◽  
Short Hairpin Rna ◽  
Carcinoma Cells ◽  
Hairpin Rna ◽  
Short Hairpin

scholarly journals Short hairpin RNA of frizzled-2 suppresses the proliferation of hepatocellular carcinoma cells

2014 ◽  
Vol 8 (4) ◽  
pp. 1519-1522 ◽  
Author(s):  
MINORU TOMIZAWA ◽  
FUMINOBU SHINOZAKI ◽  
YASUFUMI MOTOYOSHI ◽  
TAKAO SUGIYAMA ◽  
SHIGENORI YAMAMOTO ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Short Hairpin Rna ◽  
Carcinoma Cells ◽  
Hairpin Rna ◽  
Hepatocellular Carcinoma Cells ◽  
Short Hairpin

scholarly journals BRAF Silencing by Short Hairpin RNA or Chemical Blockade by PLX4032 Leads to Different Responses in Melanoma and Thyroid Carcinoma Cells

2008 ◽  
Vol 6 (5) ◽  
pp. 751-759 ◽  
Author(s):  
Elisa Sala ◽  
Luca Mologni ◽  
Silvia Truffa ◽  
Carlo Gaetano ◽  
Gideon E. Bollag ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Short Hairpin Rna ◽  
Carcinoma Cells ◽  
Hairpin Rna ◽  
Short Hairpin

scholarly journals Effect and Mechanism of Survivin on Hypoxia-Induced Multidrug Resistance of Human Laryngeal Carcinoma Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Dan Xu ◽  
Da Wei Li ◽  
Jin Xie ◽  
Xin Wei Chen
Keyword(s):  
Multidrug Resistance ◽  
Rna Interference ◽  
Cancer Cells ◽  
Propidium Iodide ◽  
Laryngeal Carcinoma ◽  
Survivin Expression ◽  
Annexin V ◽  
Carcinoma Cells ◽  
Propidium Iodide Staining ◽  
Human Laryngeal Carcinoma

This study aimed at clarifying the mechanism and role of survivin in hypoxia-induced multidrug resistance (MDR) of laryngeal carcinoma cells. Human laryngeal cancer cells were incubated under hypoxia or normoxia. The expression of survivin was silenced by performing RNA interference. Additionally, by Western blot and real-time quantitative RT-PCR, survivin expression was detected. The sensitivity of human laryngeal carcinoma cells to multiple drugs was measured by CCK-8 assay. Meanwhile, the apoptosis of cells induced by cisplatin or paclitaxel was assessed by Annexin-V/propidium iodide staining analysis. Under hypoxic conditions, the upregulation of survivin was abolished by RNA interference. Then, CCK-8 analysis demonstrated that the sensitivity to multiple agents of laryngeal carcinoma cells could be increased by inhibiting survivin expression (P<0.05). Moreover, Annexin-V/propidium iodide staining analysis revealed that decreased expression of survivin could evidently increase the apoptosis rate of laryngeal carcinoma cells that were induced by cisplatin or paclitaxel evidently (P<0.05). Our data suggests that hypoxia-elicited survivin may exert a pivotal role in regulating hypoxia-induced MDR of laryngeal cancer cells by preventing the apoptosis of cells induced by chemotherapeutic drug. Thus, blocking survivin expression in human laryngeal carcinoma cells may provide an avenue for gene therapy.

scholarly journals Inhibition of luciferase expression in hepatocellular carcinoma cells by short hairpin RNA

2004 ◽  
Vol 12 (9) ◽  
pp. 2045
Author(s):  
Li-Yi Hu ◽  
You-Shun Zhang ◽  
Xin Zhou ◽  
Zong-Qing Dai ◽  
Lin Huang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Short Hairpin Rna ◽  
Carcinoma Cells ◽  
Luciferase Expression ◽  
Hairpin Rna ◽  
Hepatocellular Carcinoma Cells ◽  
Short Hairpin
Sign in / Sign up

Export Citation Format

Share Document