Glutamic Acid Decarboxylase-Containing Axons Synapse on LHRH Neurons in the Rat Medial Preoptic Area

1985 ◽  
Vol 40 (6) ◽  
pp. 536-539 ◽  
Author(s):  
Csaba Leranth ◽  
Neil J. MacLusky ◽  
Hideki Sakamoto ◽  
Marya Shanabrough ◽  
Frederick Naftolin
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Preoptic Area ◽  
Medial Preoptic Area ◽  
Acid Decarboxylase

The use of gold-substituted silver-intensified diaminobenzidine (DAB) and non-intensified DAB for simultaneous electron microscopic immunoperoxidase labeling of tyrosine hydroxylase and glutamic acid decarboxylase immunoreactivity in the rat medial preoptic area.

1986 ◽  
Vol 34 (11) ◽  
pp. 1439-1447 ◽  
Author(s):  
T J Görcs ◽  
C Léránth ◽  
N J MacLusky
Keyword(s):  
Tyrosine Hydroxylase ◽  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Preoptic Area ◽  
Synaptic Connection ◽  
Medial Preoptic Area ◽  
Acid Decarboxylase ◽  
Electron Microscopic ◽  
Immunoperoxidase Labeling

An improved gold-substituted silver intensification procedure for the peroxidase-diaminobenzidine (DAB) reaction product was developed. The method was applied in the rat medial preoptic area to label tyrosine hydroxylase (TH)-immunoreactive profiles. Following the gold toning, the same sections were immunostained for glutamic acid decarboxylase (GAD) immunoreactivity with non-intensified peroxidase-DAB. Single DAB-labeled GAD axons were found in symmetric synaptic connection with unlabeled dendrites as well as with gold-toned immunoperoxidase-containing TH neurons.

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