Modification of Basal and GRF-Stimulated Cyclic AMP Levels and Growth Hormone Release by Phospholipid Metabolic Enzyme Inhibitors

1985 ◽  
Vol 40 (4) ◽  
pp. 332-338 ◽  
Author(s):  
Michael J. Cronin ◽  
Robert M. MacLeod ◽  
Pier L. Canonico
Keyword(s):  
Growth Hormone ◽  
Cyclic Amp ◽  
Enzyme Inhibitors ◽  
Metabolic Enzyme ◽  
Hormone Release ◽  
Growth Hormone Release

scholarly journals Increase in pituitary adenosine 3′:5′-cyclic monophosphate content and potentiation of growth-hormone release from heifer anterior pituitary slices incubated in the presence of 3-isobutyl-1-methylxanthine

1974 ◽  
Vol 142 (2) ◽  
pp. 295-300 ◽  
Author(s):  
J. George Schofield ◽  
Margaret McPherson
Keyword(s):  
Growth Hormone ◽  
Cyclic Amp ◽  
Prostaglandin E2 ◽  
Anterior Pituitary ◽  
Phosphodiesterase Inhibitor ◽  
Secretory Process ◽  
Hormone Release ◽  
Growth Hormone Release ◽  
Inhibitor Concentration ◽  
Stimulation Of

The release of growth hormone from heifer anterior pituitary slices and the cyclic AMP content of the slices were increased by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, both increases being related to inhibitor concentration over the range 0.1–1.0mm. Neither Ba2+(6.9 or 2.3mm), K+(72mm), nor p-chloromercuribenzoate (20μm) had any effect on pituitary cyclic AMP content over a 20min period. 3-Isobutyl-1-methylxanthine potentiated the release of growth hormone in response to Ba2+(2.3mm) and K+(24mm), but the degree of potentiation did not depend on inhibitor concentration in the same way as did tissue cyclic AMP content. 3-Isobutyl-1-methylxanthine decreased the concentration of K+required to give maximum stimulation of growth-hormone release, but did not significantly increase the maximum response to Ba2+. Growth-hormone release in the presence of prostaglandin E2 (1μm) was increased by 3-isobutyl-1-methylxanthine and was inhibited by the prostaglandin antagonist, 7-oxa-13-prostynoic acid, although this antagonist increased the pituitary cyclic AMP concentration and potentiated the prostaglandin E2-induced rise in cyclic AMP content. The stimulation of growth-hormone release by p-chloromercuribenzoate was not potentiated by 3-isobutyl-1-methylxanthine. The data suggest that Ba+and K+act at the same point in the secretory process as 3-isobutyl-1-methylxanthine, although by a different mechanism, and that p-chloromercuribenzoate has a different point of action.

Altered release of growth hormone from dispersed adenohypophysial cells of streptozotocin diabetic rats. II. Effects of a phorbol ester and secretagogues which increase cyclic AMP

1989 ◽  
Vol 67 (10) ◽  
pp. 1321-1325
Author(s):  
M. S. Sheppard ◽  
B. A. Eatock ◽  
R. M. Bala
Keyword(s):  
Growth Hormone ◽  
Cyclic Amp ◽  
Phorbol Ester ◽  
Phosphodiesterase Inhibitor ◽  
Adenosine Monophosphate ◽  
Diabetic Rats ◽  
Hormone Release ◽  
Growth Hormone Release ◽  
Growth Hormone Releasing Factor ◽  
Streptozotocin Diabetic Rats

We have shown in the companion paper that somatotrophs dispersed from streptozotocin diabetic rats exhibit altered sensitivity to the natural hypothalamic controlling hormones, growth hormone releasing factor and somatostatin. We have further studied the effects on growth hormone release from dispersed adenohypophysial cells of normal and streptozotocin diabetic rats of stimulation by compounds that increase cyclic 3′,5′-adenosine monophosphate formation or inhibit its breakdown and of a phorbol ester. The cells of the diabetic rats had no change in sensitivity in response to either cholera toxin or forskolin. A phosphodiesterase inhibitor caused an equal GH release from cells of both diabetic and normal animals after 60 min of incubation. There was no change in sensitivity of the cells of diabetic animals or in the maximal reponse of these cells to the phorbol ester 12-O-tetradecanoylphorbol 13-acetate when compared with normal cells. A low calcium medium that blocked growth hormone releasing factor stimulated growth hormone release from normal rat cells also blocked it from the cells of the diabetic rats. These results suggest that the defect in response of the somatotrophs of diabetic animals is specific and only occurs with the hypothalamic hormones and not with other secretagogues.Key words: growth hormone, diabetes, streptozotocin, cyclic AMP, phorbol ester.

Somatostatin Dramatically Stimulates Growth Hormone Release from Primate Somatotrophs Acting at Low Doses Via Somatostatin Receptor 5 and Cyclic AMP

2012 ◽  
Vol 24 (3) ◽  
pp. 453-463 ◽  
Author(s):  
J. Córdoba-Chacón ◽  
M. D. Gahete ◽  
M. D. Culler ◽  
J. P. Castaño ◽  
R. D. Kineman ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Cyclic Amp ◽  
Somatostatin Receptor ◽  
Low Doses ◽  
Hormone Release ◽  
Growth Hormone Release

scholarly journals Requirement for adenosine triphosphate for stimulation in vivo of ox growth-hormone release

1974 ◽  
Vol 140 (3) ◽  
pp. 479-485 ◽  
Author(s):  
Margaret McPherson ◽  
J. George Schofield
Keyword(s):  
Growth Hormone ◽  
Cyclic Amp ◽  
Prostaglandin E2 ◽  
Adenosine Triphosphate ◽  
Hormone Release ◽  
Growth Hormone Release ◽  
Atp Content ◽  
Carbonyl Cyanide ◽  
Stimulus Secretion Coupling

Rotenone, carbonyl cyanide chloromethoxyphenylhydrazone and 2,4-dinitrophenol cause parallel falls in ox pituitary ATP content and growth-hormone release in the presence of 65mm-K+. Carbonyl cyanide chloromethoxyphenylhydrazone (0.4μg/ml) prevented the rise in growth-hormone release, but not the rise in 3′:5′-cyclic AMP content after addition of prostaglandin E2 (1μg/ml). The rate of hormone release from unstimulated slices and from slices in the presence of Ba2+ at 2.3 or 6.9mm was proportional to the ATP content of the slices. Carbonyl cyanide chloromethoxyphenylhydrazone at concentrations that inhibited release in the presence of the three secretagogues did not alter the pituitary contents of Na+, K+ or Ca2+. The implications of these observations are discussed in terms of the mechanism of stimulus–secretion coupling.

Inhibitory effect of somatostatin on dibutyryl cyclic AMP-induced insulin and growth hormone release in human subjects

Metabolism ◽  
1976 ◽  
Vol 25 (3) ◽  
pp. 321-328 ◽  
Author(s):  
M. Peracchi ◽  
E. Reschini ◽  
L. Cantalamessa ◽  
A. Catania ◽  
G. Giustina
Keyword(s):  
Growth Hormone ◽  
Cyclic Amp ◽  
Human Subjects ◽  
Inhibitory Effect ◽  
Hormone Release ◽  
Growth Hormone Release ◽  
Dibutyryl Cyclic Amp

STUDIES ON THE MECHANISMS OF GROWTH-HORMONE RELEASE INDUCED BY ARGININE USING SYMPATHOLYTIC DRUGS

1969 ◽  
Vol 61 (1_Suppl) ◽  
pp. S143
Author(s):  
G. Strauch ◽  
E. Modigliani ◽  
H. Bricaire
Keyword(s):  
Growth Hormone ◽  
Hormone Release ◽  
Growth Hormone Release
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