Beta-Adrenergic Stimulation of Growth Hormone Release from Perifused Rat Anterior Pituitary Cells

1983 ◽  
Vol 37 (6) ◽  
pp. 473-475 ◽  
Author(s):  
Susan N. Perkins ◽  
William S. Evans ◽  
Michael O. Thorner ◽  
Michael.J. Cronin
Endocrinology ◽  
1986 ◽  
Vol 119 (5) ◽  
pp. 2004-2011 ◽  
Author(s):  
JACQUES SIMARD ◽  
JEAN-FRANÇOIS HUBERT ◽  
TEANOOSH HOSSEINZADEH ◽  
FERNAND LABRIE

1985 ◽  
Vol 248 (4) ◽  
pp. E438-E442
Author(s):  
A. M. Judd ◽  
K. Koike ◽  
R. M. MacLeod

Arachidonate and its metabolites increase growth hormone release in vitro. A study was designed to determine whether arachidonate release from anterior pituitary cells is modified by growth hormone-releasing factor (GRF) or somatostatin (SRIF). Cultured pituitary cells were incubated with [3H]arachidonate to esterify the long-chain fatty acid into cellular lipids. The cells were extensively washed with medium containing no [3H]arachidonate and then incubated with GRF and/or SRIF for 30 min. The incubation medium was then extracted with ethyl acetate, and following thin-layer chromatographic separation, the radioactivity in the [3H]arachidonate band was measured. GRF in a concentration-dependent manner (1-30 nM) stimulated growth hormone and arachidonate release, whereas SRIF (100 nM) blocked the GRF-induced increase of growth hormone and arachidonate release. The effects of GRF on growth hormone and arachidonate were evident at time intervals as brief as 5 min. These findings support the hypothesis that arachidonate may play a role in the GRF-induced growth hormone release.


1990 ◽  
Vol 40 (4) ◽  
pp. 887-892 ◽  
Author(s):  
Iván Nagy ◽  
Gábor B. Makara ◽  
Vera Garamvölgyi ◽  
Sándor Bajusz ◽  
Erzsébet Széll ◽  
...  

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