Immunocytochemical Localization of Luteinizing Hormone-Releasing Hormone in Male and Female Rat Brains

1983 ◽  
Vol 36 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Brenda D. Shivers ◽  
Richard E. Harlan ◽  
Joan I. Morrell ◽  
Donald W. Pfaff
Keyword(s):  
Luteinizing Hormone ◽  
Female Rat ◽  
Immunocytochemical Localization ◽  
Male And Female ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone

EFFECTS OF LUTEINIZING HORMONE RELEASING HORMONE ON THE ULTRASTRUCTURE OF GONADOTROPHS IN THE FOETUS OF THE RHESUS MONKEY NEAR TERM

1977 ◽  
Vol 74 (3) ◽  
pp. 369-373 ◽  
Author(s):  
M. T. TSENG
Keyword(s):  
Luteinizing Hormone ◽  
Rhesus Monkeys ◽  
Secretory Granules ◽  
Nuclear Chromatin ◽  
Male And Female ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Cytoplasmic Vesicles ◽  
Near Term ◽  
Lh Rh

SUMMARY Eighty micrograms of synthetic luteinizing hormone releasing hormone (LH-RH) were infused systemically into male and female foetuses of rhesus monkeys near term. Control animals were given infusions of saline. Morphologically, the control gonadotrophs varied from cells filled with secretory granules to highly stimulated cells with numerous cytoplasmic vesicles. In the LH-RH-treated animals, however, many cells showed depletion of secretory granules, dilatation of the endoplasmic reticulum and condensed nuclear chromatin. It is concluded, therefore, that foetal gonadotrophs can respond to administration of synthetic LH-RH.

THE PRESENCE OF PEPTIDASES IN THE RAT HYPOTHALAMUS INACTIVATING LUTEINIZING HORMONE RELEASING HORMONE (LH-RH)

1974 ◽  
Vol 77 (3) ◽  
pp. 435-442 ◽  
Author(s):  
E. C. Griffiths ◽  
K. C. Hooper ◽  
S. L. Jeffcoate ◽  
D. T. Holland
Keyword(s):  
Luteinizing Hormone ◽  
Reproductive Function ◽  
Assay Method ◽  
Supernatant Fraction ◽  
Peptidase Activity ◽  
Female Rat ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Rat Hypothalamus ◽  
Lh Rh

ABSTRACT The presence of peptidases in the rat hypothalamus inactivating luteinizing hormone-releasing hormone (LH-RH) has previously been demonstrated using an indirect assay method. With the development of a sensitive and specific radioimmunoassay for the releasing hormone, this technique was employed in the study of the peptidases inactivating LH-RH. It was found that supernatant fractions from both male and female rat hypothalami rapidly inactivated LH-RH, and that the peptidase activity of the supernatant fraction was higher in male than in female animals though the particulate fraction's activity was about the same in both sexes. Peptidase activity was also considerably greater in the supernatant than in the particulate fraction. These results confirm that the hypothalamus contains peptidases capable of inactivating LH-RH and give a direct indication that the enzymes may be involved in the central nervous system's control of reproductive function.

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