Impermeability of the Rat Blood-Brain Barrier to Exogenously Administered Gonadotropin-Releasing Hormone

1983 ◽  
Vol 36 (2) ◽  
pp. 102-104 ◽  
Author(s):  
Christian Verheugen ◽  
Larry R. Laufer ◽  
John L. DeFazio ◽  
William B. Pardridge ◽  
John K.H. Lu ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Gonadotropin Releasing Hormone ◽  
Brain Barrier ◽  
Releasing Hormone ◽  
Rat Blood ◽  
Blood Brain

scholarly journals The Effect of Bile Salts on the Permeability and Ultrastructure of the Perfused, Energy-Depleted, Rat Blood-Brain Barrier

1991 ◽  
Vol 11 (4) ◽  
pp. 644-654 ◽  
Author(s):  
J. Greenwood ◽  
J. Adu ◽  
A. J. Davey ◽  
N. J. Abbott ◽  
M. W. B. Bradbury
Keyword(s):  
Blood Brain Barrier ◽  
Bile Salts ◽  
Structural Changes ◽  
Sodium Deoxycholate ◽  
Ringer Solution ◽  
Brain Barrier ◽  
Rat Blood ◽  
Blood Brain ◽  
Ultrastructural Damage

The action of bile salts upon the rat blood–brain barrier (BBB) was assessed in the absence of energy-yielding metabolism. Brains were perfused in situ with a Ringer solution for 5 min followed by a 1 min perfusion containing either sodium deoxycholate (DOC), taurochenodeoxycholate (TCDC), or Ringer/DNP. The integrity of the BBB was then determined by perfusing with the radiotracer [14C]mannitol for 2.5 min. Alternatively, the brains were perfusion fixed for ultrastructural assessment. At 0.2 m M DOC, the BBB remained intact and the cerebral ultrastructure was similar to the controls. At 1 m M and above, disruption of the BBB became evident. At 2 m M, the cerebral cortex became severely vacuolated, with damaged endothelium and collapsed capillaries. With TCDC, BBB disruption occurred at 0.2 m M without any apparent ultrastructural damage to the micro vasculature. Following 2 m M TCDC, similar, but less widespread, structural changes to the 2 m M DOC-perfused animals was apparent. Opening of the BBB occurred at a concentration lower than that required to cause lysis of either red blood cells or cultured cerebral endothelial cells. It is proposed that the effect of bile salts at concentrations of 1.5 m M and above is largely due to their lytic action as strong detergents on endothelial cell membranes, but that at lower concentrations a more subtle modification of the BBB occurs.

scholarly journals Temporary disruption of the rat blood–brain barrier with a monoclonal antibody: A novel method for dynamic manganese-enhanced MRI

NeuroImage ◽  
2010 ◽  
Vol 50 (1) ◽  
pp. 7-14 ◽  
Author(s):  
Hanbing Lu ◽  
Steven Demny ◽  
Yantao Zuo ◽  
William Rea ◽  
Leiming Wang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Rat Blood ◽  
Blood Brain ◽  
Enhanced Mri ◽  
Novel Method ◽  
Manganese Enhanced Mri
Sign in / Sign up

Export Citation Format

Share Document