Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells

2008 ◽  
Vol 188 (3) ◽  
pp. 251-258 ◽  
Author(s):  
Kellie J. Williams ◽  
Alicia A. Picou ◽  
Sharon L. Kish ◽  
Angelica M. Giraldo ◽  
Robert A. Godke ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Adult Stem Cells ◽  
Isolation And Characterization

Isolation and characterization of exosomes from adipose tissue‐derived mesenchymal stem cells

2020 ◽  
Author(s):  
Elsa González‐Cubero ◽  
María Luisa González‐Fernández ◽  
Laura Gutiérrez‐Velasco ◽  
Eliezer Navarro‐Ramírez ◽  
Vega Villar‐Suárez
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Isolation And Characterization

Isolation and Characterization of Adult Stem Cells from Human Salivary Glands

2008 ◽  
Vol 17 (3) ◽  
pp. 509-518 ◽  
Author(s):  
Nicole Rotter ◽  
Jessica Oder ◽  
Peter Schlenke ◽  
Ulrich Lindner ◽  
Florian Böhrnsen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Salivary Glands ◽  
Adult Stem Cells ◽  
Isolation And Characterization

scholarly journals ISOLATION AND CHARACTERIZATION OF ADULT STEM CELLS FROM BONE MARROW

2012 ◽  
Vol 7 (1) ◽  
pp. 215-223
Author(s):  
Zahran F. ◽  
EL-Deen.I. M ◽  
Salama. M ◽  
Lotfy. A
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adult Stem Cells ◽  
Isolation And Characterization

scholarly journals Comparison of biological features of wild European rabbit mesenchymal stem cells derived from different tissues.

2021 ◽  
Author(s):  
Marta Díaz-de Frutos ◽  
Alexandra Calle ◽  
María Zamora-Ceballos ◽  
Juan Bárcena ◽  
Esther Blanco ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
European Rabbit ◽  
Isolation And Characterization ◽  
Expression Characteristic ◽  
Subcutaneous Adipose ◽  
Different Tissues

Although the European rabbit is an "endangered" species and a notorious biological model, the analysis and comparative characterization of new tissue sources of rabbit mesenchymal stem cells (rMSCs) has not been well studied. Here we report for the first time the isolation and characterization of rMSCs derived from an animal belonging to a natural rabbit population within the species native region. New rMSC lines were isolated from different tissues: oral mucosa (rOM-MSC), dermal skin (rDS-MSC), subcutaneous adipose tissue (rSCA-MSC), ovarian adipose tissue (rOA-MSC), oviduct (rO-MSC), and mammary gland (rMG­MSC). The six rMSC lines showed plastic adhesion with fibroblast-like morphology and were all shown to be positive for CD44 and CD29 expression (characteristic markers of MSCs), and negative for CD34 or CD45 expression. In terms of pluripotency features, all rMSC lines expressed NANOG, OCT4, and SOX2. Furthermore, all rMSC lines cultured under osteogenic, chondrogenic, and adipogenic conditions showed differentiation capacity. In conclusion, this study describes the isolation and characterization of new rabbit cell lines from different tissue origins, with a clear mesenchymal pattern. We show that rMSC do not exhibit differences in terms of morphological features, expression of the cell surface, and intracellular markers of pluripotency and in vitro differentiation capacities, attributable to their tissue of origin.

285 ISOLATION AND CHARACTERIZATION OF BOVINE ADIPOSE-DERIVED SOMATIC STEM CELLS

2009 ◽  
Vol 21 (1) ◽  
pp. 239 ◽  
Author(s):  
A. A. Picou ◽  
R. A. MacLean ◽  
B. Dresser ◽  
R. A. Godke ◽  
K. R. Bondioli
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Adult Stem Cells ◽  
Enzyme Concentration ◽  
Average Cell ◽  
Isolation And Characterization ◽  
Donor Cells ◽  
Passage Number ◽  
Culture Duration

Adipose tissue has proven to serve as an abundant and rich source of adult stem cells with pluripotent properties. It has been suggested that pluri potent cell could serve as en efficient source of donor cells for nuclear transfer. The objective of this study was to develop an isolation and culture protocol for bovine adipose tissue-derived stem cells Tissue was collected post-mortem from the brisket of cows at a local abattoir. For cell isolation, the tissue was finely minced and weighed. The tissue was washed two times with PBS plus 2% penicillin-streptomycin (P/S) and enzymatically digested in a collagenase type 1 solution. Isolation conditions were determined by using a 3 × 3 factorial design. The tissue was digested in PBS with 0.1%, 0.25%, or 0.50% collagenase type 1 and 1% BSA, 2% P/S, and 2% Fungazone. Each enzyme concentration was incubated for 1, 2, or 3 hours in a shaking incubator at 39°C. The cellular mixtures were centrifuged at 300g for 5 minutes, floating adipocytes removed, and the remaining cell suspension passed through filter apparatus consisting of 180 and 20 μm nylon meshes. Cells were resuspended in PBS plus 1% BSA and centrifuged again for 5 minutes at 300g. Pelleted stromal cells were washed and nucleated cells were counted using Hoechst stain at a concentration of 2 μg mL–1. Primary cell cultures were plated in a 12.5 cm2 culture flask in DMEM (high glucose) supplemented with 10%FBS and 1% P/S. Cells were cultured for 48 h in a 38°C incubator in a humidified atmosphere of 5% CO2 in air. Nonadherent cells were removed by washing with PBS. For some cultures adherent cells were removed by trypsinization to determine the proportion of cell adhering. On average 23.38% of cells remained adherent, and the average cell cycle length was 1.85 days. Table 1 displays the average number of nucleated cells that were released per gram of fat for the various collagenase concentrations and exposure times. Analysis of data by one-way ANOVA show no differences. Growth factor supplementation is currently being evaluated on the effects of culture duration and passage number. Isolation conditions of 0.25% collagenase for 2 hours incubation has been consistently successful at obtaining viable ADAS cultures from cattle and common eland (Taurotragus oryx). Table 1.Average number of cells released and viability after collagenase treatments Funding provided by LSU/ACRES collaborative research program.

Sign in / Sign up

Export Citation Format

Share Document