Proteasome Inhibition Potentiates the Cytotoxic Effects of Hyperthermia in HT-29 Colon Cancer Cells through Inhibition of Heat Shock Protein 27

Oncology ◽  
2007 ◽  
Vol 73 (1-2) ◽  
pp. 98-103 ◽  
Author(s):  
Fei Chen ◽  
Reza Rezavi ◽  
Cha-Chi Wang ◽  
Lawrence E. Harrison
Keyword(s):  
Colon Cancer ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Cancer Cells ◽  
Proteasome Inhibition ◽  
Heat Shock Protein 27 ◽  
Colon Cancer Cells ◽  
Cytotoxic Effects ◽  
Ht 29

scholarly journals Suppression of heat shock protein 27 expression promotes 5-fluorouracil sensitivity in colon cancer cells in a xenograft model

2012 ◽  
Vol 28 (4) ◽  
pp. 1269-1274 ◽  
Author(s):  
RYOHEI HAYASHI ◽  
YOSHIYUKI ISHII ◽  
HIROKI OCHIAI ◽  
ATSUSHI MATSUNAGA ◽  
TAKASHI ENDO ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Cancer Cells ◽  
Xenograft Model ◽  
Heat Shock Protein 27 ◽  
Colon Cancer Cells

Inhibition of Heat-shock Protein 27 Reduces 5-Fluorouracil-acquired Resistance in Human Colon Cancer Cells

2021 ◽  
Vol 41 (3) ◽  
pp. 1283-1290
Author(s):  
YUSUKE ASADA ◽  
MASASHI TSURUTA ◽  
KOJI OKABAYASHI ◽  
KOHEI SHIGETA ◽  
TAKASHI ISHIDA ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Cancer Cells ◽  
Acquired Resistance ◽  
Human Colon ◽  
Heat Shock Protein 27 ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells

Heat shock protein 27 influences the anti-cancer effect of curcumin in colon cancer cells through ROS production and autophagy activation

Life Sciences ◽  
2018 ◽  
Vol 209 ◽  
pp. 43-51 ◽  
Author(s):  
Hung-Hua Liang ◽  
Chien-Yu Huang ◽  
Ching-Wen Chou ◽  
Precious Takondwa Makondi ◽  
Ming-Te Huang ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Cancer Cells ◽  
Heat Shock Protein 27 ◽  
Ros Production ◽  
Colon Cancer Cells ◽  
Anti Cancer

scholarly journals The Heat Shock Protein 70 is a Novel Target for Nobiletin in Human Colon Cancer Cells

2016 ◽  
Vol 30 (S1) ◽  
Author(s):  
Zili Gao ◽  
Jinkai Zheng ◽  
Xian Wu ◽  
Kalina Dimova ◽  
Stylianos Scordilis ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Cancer Cells ◽  
Heat Shock Protein 70 ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Novel Target ◽  
Human Colon Cancer Cells

Assessment of anti-cancer effects of koenimbine on colon cancer cells

2020 ◽  
Vol 28 (3) ◽  
pp. 185-190
Author(s):  
Maliheh Astaneh ◽  
Soudeh Ghafouri-Fard ◽  
Zahra Fazeli ◽  
Zahra Taherian-Esfahani ◽  
Sepideh Dashti ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Annexin V ◽  
Colon Cancer Cells ◽  
Cytotoxic Effects ◽  
Anti Cancer ◽  
Ht 29

BACKGROUND: Recent studies have highlighted the role of natural elements in reduction of cancer cell growth and apoptosis. Koenimbine, a natural product isolated from Murraya koenigii (L) Spreng is a substance with cytotoxic effects on cancer cells. AIM: The effects of koenimbine on HT-29 and SW48 colon cancer cells were evaluated by MTT and Annexin V assays. Expression levels of Wnt/β-catenin pathway genes were quantified by real time PCR. RESULTS: The IC50 values of koenimbine in HT-29 and SW48 was calculated to be 50 μg/ml based on the results of MTT assay. This value was 75 μg/ml in IEC-18 cells which were used as normal control. Annexin V assays revealed induction of cell apoptosis and necrosis in HT-29 and SW48 cells but not IEG18 cells by koenimbine. Koenimbin treatment resulted in significant down-regulation of CYCLD1 expression in SW48 cell line, but up-regulation of this gene in HT29 cell line. Expression of TBLR1, DKK1, GSK3B and β-catenin was significantly decreased after koenimbin treatment in HT-19 cell line. Moreover, expression of DKK1 and GSK3B was significantly decreased after koenimbin treatment in SW-40 cell line. TCF4 expression was not detected in any of cell lines either before or after treatment with koenimbin. CONCLUSION: The current in vitro study showed the cytotoxic effects of koenimbin on two colon cancer cell lines and the effects of this substance on expression of selected genes from Wnt-β catenin pathway. Future in vivo studies are needed before suggestion of this substance as an anti-cancer drug.

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