Effects of Carbamazepine on Action Potentials and Calcium Currents in Rat Spinal Ganglion Cells in vitro

1993 ◽  
Vol 27 (3) ◽  
pp. 176-179 ◽  
Author(s):  
K. Schirrmacher ◽  
A. Mayer ◽  
J. Walden ◽  
R. Düsing ◽  
D. Bingmann
Keyword(s):  
Action Potentials ◽  
Ganglion Cells ◽  
Spinal Ganglion ◽  
Calcium Currents ◽  
Spinal Ganglion Cells

Effects of carbamazepine on membrane properties of rat sensory spinal ganglion cells in vitro

1995 ◽  
Vol 5 (4) ◽  
pp. 501-507 ◽  
Author(s):  
K. Schirrmacher ◽  
A. Mayer ◽  
J. Walden ◽  
R. Düsing ◽  
D. Bingmann
Keyword(s):  
Ganglion Cells ◽  
Spinal Ganglion ◽  
Membrane Properties ◽  
Spinal Ganglion Cells

scholarly journals THE NISSL SUBSTANCE OF LIVING AND FIXED SPINAL GANGLION CELLS

1957 ◽  
Vol 3 (3) ◽  
pp. 449-456 ◽  
Author(s):  
Arline D. Deitch ◽  
Montrose J. Moses
Keyword(s):  
Ganglion Cells ◽  
Spinal Ganglion ◽  
Basic Dyes ◽  
Spinal Ganglion Cells ◽  
Minimal Damage ◽  
High Concentrations ◽  
Spinal Ganglion Neurons ◽  
Living Neurons ◽  
Ganglion Neurons

Living chick spinal ganglion neurons grown for 19 to 25 days in vitro were photographed with a color-translating ultraviolet microscope (UV-91) at 265, 287, and 310 mµ. This instrument was unique in permitting rapid accumulation of ultraviolet information with minimal damage to the cell. In the photographs taken at 265 mµ of the living neurons, discrete ultraviolet-absorbing cytoplasmic masses were observed which were found to be virtually unchanged in appearance after formalin fixation. These were identical with the Nissl bodies of the same cells seen after staining with basic dyes. The correlation of ultraviolet absorption, ribonuclease extraction, and staining experiments with acid and basic dyes confirmed the ribonucleoprotein nature of these Nissl bodies in the living and fixed cells. No change in distribution or concentration of ultraviolet-absorbing substance was observed in the first 12 ultraviolet photographs of a neuron, and it is concluded that the cells had not been subjected to significant ultraviolet damage during the period of photography. On the basis of these observations, as well as previous findings with phase contrast microscopy, it is concluded that Nissl bodies preexist in the living neuron as discrete aggregates containing high concentrations of nucleoprotein.

scholarly journals THE NISSL SUBSTANCE OF LIVING AND FIXED SPINAL GANGLION CELLS

1956 ◽  
Vol 2 (4) ◽  
pp. 433-444 ◽  
Author(s):  
Arline D. Deitch ◽  
Margaret R. Murray
Keyword(s):  
Living Cell ◽  
Phase Contrast ◽  
Ganglion Cells ◽  
Spinal Ganglion ◽  
Spinal Ganglion Cells ◽  
Cell Dehydration ◽  
Spinal Ganglion Neurons ◽  
Ganglion Neurons ◽  
Nissl Substance

Living chick embryo spinal ganglion neurons grown from 1 to 4 weeks in vitro were studied under the phase contrast microscope. In the peripheral cytoplasm of the earliest stages studied, a homogeneous, phase-dense material is seen which corresponds in location to the cytoplasmic basophil material of the same stages. As maturation proceeds, this material increases in extent, and becomes separated by lighter channels into discrete bodies. Short fixation by 1 per cent buffered osmium tetroxide followed by post-fixation with neutral buffered formalin does not significantly alter the size, shape, or distribution of any of the cytoplasmic components, and the fixed, hydrated cell is almost indistinguishable from the living cell. Dehydration causes some shrinkage of the fixed preparations, but if the photographs of the stained preparations are enlarged to correspond with those of the living cell, excellent correspondence can be made between at least the larger basophil masses and the larger dark masses seen with phase contrast. Fixation by a formalin-mercuric chloride procedure also results in satisfactory correspondence between the stained Nissl bodies and the phase-dark homogeneous areas. It is concluded that discrete Nissl bodies preexist in the living neuron and are essentially unchanged after good cytological fixation. Evidence is also presented of the presence of neurofibrils in the living state.

Difference in phosphorylation of neurofilament proteins in motor neurons and spinal ganglion cells

1989 ◽  
Vol 15 (4) ◽  
pp. 467-473 ◽  
Author(s):  
I TOYOSHIMA ◽  
A YAMAMOTO ◽  
O MASAMUNE ◽  
S YAMADA ◽  
M SATAKE
Keyword(s):  
Motor Neurons ◽  
Ganglion Cells ◽  
Spinal Ganglion ◽  
Neurofilament Proteins ◽  
Spinal Ganglion Cells

scholarly journals Electron Microscopic Observations on Spinal Ganglion Cells of Rana pipiens after Injection of Malononitrile

1958 ◽  
Vol 4 (1) ◽  
pp. 83-86 ◽  
Author(s):  
Everett Anderson ◽  
V. L. van Breemen
Keyword(s):  
Golgi Complex ◽  
Rana Pipiens ◽  
Ganglion Cells ◽  
Morphological Changes ◽  
Membrane System ◽  
Spinal Ganglion ◽  
Electron Microscopic ◽  
Biochemical Alterations ◽  
Spinal Ganglion Cells ◽  
Electron Microscopes

Spinal ganglionic cells of Rana pipiens were studied with light and electron microscopes in normal animals and in animals which had received graded dosages of malononitrile intraperitoneally. After treatment no increase in the intensity of staining was noted in the Nissl substance when spinal ganglion cells were examined with the light microscope. The electron micrographs demonstrated the following in malononitrile-treated animals: 1. The cisternae of the endoplasmic reticulum composing the Nissl bodies appeared to fragment and lose their parallel orientation. 2. The microvesicular components of the Golgi complex appeared to increase in number, and the increase was apparently due to fragmentation of the membrane system of the Golgi complex. 3. The mitochondria enlarged and became pleomorphic, but displayed no alterations of internal structure. The morphological changes may be interpreted as reflections of biochemical alterations.

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