L-Tyrosine and L-Tryptophan Transport in Red Blood Cells in Normal Subjects

1986 ◽  
Vol 15 (1) ◽  
pp. 7-12 ◽  
Author(s):  
J. Widmer ◽  
J.-M. Gaillard ◽  
R. Tissot
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Normal Subjects ◽  
Tryptophan Transport

Catechol O-Methyltransferase in Red Blood Cells of Schizophrenic, Depressed, and Normal Human Subjects

1976 ◽  
Vol 128 (2) ◽  
pp. 184-187 ◽  
Author(s):  
Helen L. White ◽  
Malcolm N. McLeod ◽  
Jonathan R. T. Davidson
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Subjects ◽  
Normal Subjects ◽  
Enzyme Preparations ◽  
Human Red Blood Cells ◽  
Schizophrenic Patients ◽  
Normal Human ◽  
The Mean ◽  
Isoelectric Ph

SummaryCatechol O-methyltransferase of lysed human red blood cells was assayed under optimal conditions, using saturating concentrations of the substrates, S-adenosyl-L-methionine and 3,4-dihydroxybenzoic acid. The mean enzyme activity found in 24 normal subjects was 29.2 nmol/hr/ml RBC. The mean activity in blood of 33 female unipolar depressives was not significantly different from normal. However, higher enzyme activities were observed in the blood of 11 schizophrenic patients (38.9 nmol/hr/ml RBC). Partially purified enzyme preparations from blood of normal and schizophrenic individuals were indistinguishable with respect to substrate specificities, isoelectric pH values, and ratios of the two O-methylated products. Therefore it is unlikely that any defect in O-methylation which may occur in schizophrenia can be attributed to a change in the intrinsic properties of erythrocyte catechol O-methyltransferase.

Estimation of pore passage time of red blood cells in normal subjects and patients with renal failure

1980 ◽  
Vol 36 (3) ◽  
pp. 325-327 ◽  
Author(s):  
Y. Kikuchi ◽  
M. Horimoto ◽  
T. Koyama ◽  
Y. Koyama ◽  
S. Tozawa
Keyword(s):  
Renal Failure ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Normal Subjects ◽  
Passage Time

Detection of CD55- and CD59-Negative Immature Reticulocytes May Improves Sensitivity/Specificity to Identify a Minor Population of PNH-Type Cells in Patients with Myelodysplastic Syndrome/Aplastic Anemia

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 4373-4373
Author(s):  
Naoshi Obara ◽  
Shoko Satoh ◽  
Yasushi Okoshi ◽  
Shigeru Chiba ◽  
Haruhiko Ninomiya
Keyword(s):  
Myelodysplastic Syndrome ◽  
Red Blood Cells ◽  
Aplastic Anemia ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Mononuclear Cells ◽  
Conflicts Of Interest ◽  
Normal Subjects ◽  
Good Alternative ◽  
Minor Population

Abstract Abstract 4373 [Background] Paroxysmal nocturnal hemogloblinuria (PNH) is a hemolytic disease characterized by complement-sensitive red blood cells (RBC). Sometimes, a tiny amount of PNH-type, i.e., CD55- or CD59-negative, red blood cells as well as neutrophils can be detected in peripheral blood from patients with myelodysplastic syndrome (MDS)/ aplastic anemia (AA). It has been reported that the presence of PNH-type cells is correlated with a good response to immunosuppressive therapy foe these disease. However, the ratio of PNH-type RBCs to normal RBCs is always underestimated because PNH-type red blood cells have a shortened life span in the circulation, and there are some patients who have very low number of circulating granulocytes. [Metods] Peripheral blood was obtained from 37 patients with MDS or AA and 30 normal subjects. Mononuclear cells were then stained with antibodies against CD55, CD59, and CD71, and analyzed by a flowcytometer. Subsequently, CD55 and CD59-negative and CD71-positive reticulocytes, representing an immature fraction of reticulocytes, were identified. Sensitivity and specificity were compared for the detection of PNH-type immature reticulocytes with those evaluated by a PNH-type RBC- and granulocyte-detecting method. [Results and discussion] All 30 normal subjects were negative when the cut-off value for CD55 and CD59-negative and CD71-positive reticulocytes was set at 0.008%. With this cut-off value, PNH-type immature reticulocytes were detected in 18 out of 37 MSA/AA patients. When the cut-off values for PNH-type RBCs and granulocytes were set at 0.005% and 0.01%, respectively, in accordance with the original report by Sugimori, et al, 16 and 18 cases among 37 MDS/AA patients were positive for PNH-type RBCs and granulocytes, respectively. All the 16 cases positive for PNH-type RBCs were also positive for PNH-type granulocytes and immature reticulocytes, while 2 cases negative for PNH-type RBCs were evaluated to be positive for both PNH-type granulocytes and immature reticulocytes. All the cases positive for granulocytes were also positive for immature reticulocytes, and vice versa. These observations indicate that the method to detect minor population of PNH-type immature reticulocytes is feasible and comparable with that to detect PNH-type granulocytes, and could be superior to the PNH-type RBC-detection method in terms of sensitivity, without reducing specificity. If the evaluation of PNH-type granulocytes is difficult, PNH-type immature reticulocytes may be a good alternative to be evaluated. Disclosures: No relevant conflicts of interest to declare.

THE EFFECT OF RED BLOOD CELLS ON PLATELET AGGREGATION IN NORMAL SUBJECTS AND MYELOFIBROTIC PATIENTS

1987 ◽  
Author(s):  
C L Balduini ◽  
G Bertolino ◽  
G Polino ◽  
G Gamba ◽  
F Sinigaglia ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Optical Method ◽  
Maximum Rate ◽  
Pathological Condition ◽  
Normal Subjects ◽  
High Concentration

We investigated the effect of red blood cells (RBC) on "in vitro" platelet aggregation by the use of the "Electronic Whole Blood Aggregometer" (Chrono-Log Corporation). Preliminary experiments, studying platelet aggregation in the same PRP by the simultaneous use of the optical method and the electronic method, demonstrated that the maximum rate of impedance changes corre lated well with both the rate and the extent of platelet aggregation as measured by the optical method. The refore, the maximum rate of impedance increase was cho sen for the measurement of platelet aggregation in the presence of different concentrations of RBC. RBC, both at 40 and 60%, significantly inhibited platelet aggregation stimulated by low and high concentration of ADP and epinephrine. Platelet aggregation stimulated by co llagen was slightly reduced only by the higher RBC con centration. The effect of RBC on platelet aggregation was also investigated in idiopathic myelofibrosis, a pathological condition characterized by both platelet and RBC alterations. While on the basis of PRP studies 5 out of 17 patients had hypo-aggregation and 12 had normal aggregation, whole blood studies evidentiated hypo-aggregation in 3 patients, normal aggregation in 4 and spontaneous platelet aggregation (SPA) in 10. SPA was a consequence of platelet abnormality, since it occurred also when platelets from patients were sti rred with normal RBC.In conclusion, RBC may exert different effects on the aggregation of normal and pathological platelets.

l-Tryptophan transport in human red blood cells

1980 ◽  
Vol 598 (2) ◽  
pp. 375-384 ◽  
Author(s):  
Raben Rosenberg ◽  
James D. Young ◽  
J. Clive Ellory
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Red Blood Cells ◽  
Tryptophan Transport

Diphenylhydantoin binding by red blood cells of normal subjects

Neurology ◽  
1974 ◽  
Vol 24 (6) ◽  
pp. 575-575 ◽  
Author(s):  
P. HANSOTIA ◽  
E. KEAAN
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Normal Subjects

Role of chloride in potassium transport through a K-Cl cotransport system in human red blood cells

1989 ◽  
Vol 256 (5) ◽  
pp. C994-C1003 ◽  
Author(s):  
C. Brugnara ◽  
T. Van Ha ◽  
D. C. Tosteson
Keyword(s):  
Membrane Potential ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Normal Subjects ◽  
Similar Data ◽  
Human Red Blood Cells ◽  
Dense Fraction ◽  
Cotransport System ◽  
Low K

In this paper, we report experiments demonstrating the coupling of Cl and K movements in a volume-dependent K-Cl cotransport system in human red blood cells. We show that an outwardly directed Cl gradient can promote net K efflux against an inwardly directed K gradient at constant membrane potential. Red blood cell membrane potential was kept constant by using anions that are not transported through the K-Cl cotransport system but that are more permeable than Cl (NO3 and SCN). Under these conditions, when the activities of band 3 (capnophorin)-mediated anion exchange and of the carbonic anhydrase have been inhibited, it is possible to maintain a Cl gradient at constant membrane potential. Similar data were obtained in human red blood cells (least dense fraction from normal subjects and whole blood from patients with homozygous hemoglobin S disease), in rabbit red blood cells, and in low-K sheep red blood cells. These data confirm that the volume-dependent Cl-dependent K movement in these cells operates through coupled K-Cl cotransport.

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