Gene Expression Analysis in Superficial Bladder Cancer: Comparison of Two Suitable Endogenous Reference Genes

2007 ◽  
Vol 1 (3) ◽  
pp. 145-150 ◽  
Author(s):  
Paula A. Videira ◽  
Dário Ligeiro ◽  
Manuela Correia ◽  
Hélder Trindade
Keyword(s):  
Gene Expression ◽  
Bladder Cancer ◽  
Expression Analysis ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Superficial Bladder Cancer ◽  
Endogenous Reference ◽  
Endogenous Reference Genes

scholarly journals Identification of Endogenous Reference Genes for RT-qPCR Expression Analysis in Urochloa brizantha Under Abiotic Stresses

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Luciana Midori Takamori ◽  
Alyne Valéria Carrion Pereira ◽  
Gustavo Maia Souza ◽  
Luiz Gonzaga Esteves Vieira ◽  
Alessandra Ferreira Ribas
Keyword(s):  
Expression Analysis ◽  
Abiotic Stresses ◽  
Reference Genes ◽  
Endogenous Reference ◽  
Urochloa Brizantha ◽  
Endogenous Reference Genes

scholarly journals Validation of Internal Control Genes for Quantitative Real-Time PCR Gene Expression Analysis in Morchella

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2331 ◽  
Author(s):  
Qianqian Zhang ◽  
Wei Liu ◽  
Yingli Cai ◽  
A-Feng Lan ◽  
Yinbing Bian
Keyword(s):  
Gene Expression ◽  
Candidate Genes ◽  
Expression Analysis ◽  
Internal Control ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Stable Gene ◽  
Experimental Conditions ◽  
The Stability ◽  
Vacuolar Protein

The reliability of qRT-PCR results depend on the stability of reference genes used for normalization, suggesting the necessity of identification of reference genes before gene expression analysis. Morels are edible mushrooms well-known across the world and highly prized by many culinary kitchens. Here, several candidate genes were selected and designed according to the Morchella importuna transcriptome data. The stability of the candidate genes was evaluated with geNorm and NormFinder under three different experimental conditions, and several genes with excellent stability were selected. The extensive adaptability of the selected genes was tested in ten Morchella species. Results from the three experimental conditions revealed that ACT1 and INTF7 were the most prominent genes in Morchella, CYC3 was the most stable gene in different development stages, INTF4/AEF3 were the top-ranked genes across carbon sources, while INTF3/CYC3 pair showed the robust stability for temperature stress treatment. We suggest using ACT1, AEF3, CYC3, INTF3, INTF4 and INTF7 as reference genes for gene expression analysis studies for any of the 10 Morchella strains tested in this study. The stability and practicality of the gene, vacuolar protein sorting (INTF3), vacuolar ATP synthase (INTF4) and14-3-3 protein (INTF7) involving the basic biological processes were validated for the first time as the candidate reference genes for quantitative PCR. Furthermore, the stability of the reference genes was found to vary under the three different experimental conditions, indicating the importance of identifying specific reference genes for particular conditions.

Comprehensive gene expression analysis after ERH gene knockdown in human bladder cancer T24 cell lines

Gene ◽  
2020 ◽  
Vol 738 ◽  
pp. 144475
Author(s):  
Kun Pang ◽  
Lin Hao ◽  
Zhenduo Shi ◽  
Bo Chen ◽  
Huiqing Pang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bladder Cancer ◽  
Cell Lines ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Gene Knockdown ◽  
Human Bladder Cancer ◽  
Human Bladder
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