Potentiation of Potassium Currents by Beta-Adrenoceptor Agonists in Human Urinary Bladder Smooth Muscle Cells: A Possible Electrical Mechanism of Relaxation

Pharmacology ◽  
2008 ◽  
Vol 81 (3) ◽  
pp. 251-258 ◽  
Author(s):  
Jun Takemoto ◽  
Haruko Masumiya ◽  
Kazuo Nunoki ◽  
Takeya Sato ◽  
Haruo Nakagawa ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Bladder Smooth Muscle ◽  
Human Urinary Bladder ◽  
Beta Adrenoceptor ◽  
Urinary Bladder Smooth Muscle ◽  
Adrenoceptor Agonists ◽  
Beta Adrenoceptor Agonists

scholarly journals Stretch-induced Calcium Release in Smooth Muscle

2002 ◽  
Vol 119 (6) ◽  
pp. 533-543 ◽  
Author(s):  
Guangju Ji ◽  
Robert J. Barsotti ◽  
Morris E. Feldman ◽  
Michael I. Kotlikoff
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Smooth Muscle Cells ◽  
Calcium Release ◽  
Muscle Cells ◽  
Intraluminal Pressure ◽  
Bladder Smooth Muscle ◽  
Urinary Bladder Smooth Muscle ◽  
Inward Currents ◽  
Longitudinal Stretch

Smooth muscle cells undergo substantial increases in length, passively stretching during increases in intraluminal pressure in vessels and hollow organs. Active contractile responses to counteract increased transmural pressure were first described almost a century ago (Bayliss, 1902) and several mechanisms have been advanced to explain this phenomenon. We report here that elongation of smooth muscle cells results in ryanodine receptor–mediated Ca2+ release in individual myocytes. Mechanical elongation of isolated, single urinary bladder myocytes to ∼120% of slack length (ΔL = 20) evoked Ca2+ release from intracellular stores in the form of single Ca2+ sparks and propagated Ca2+ waves. Ca2+ release was not due to calcium-induced calcium release, as release was observed in Ca2+-free extracellular solution and when free Ca2+ ions in the cytosol were strongly buffered to prevent increases in [Ca2+]i. Stretch-induced calcium release (SICR) was not affected by inhibition of InsP3R-mediated Ca2+ release, but was completely blocked by ryanodine. Release occurred in the absence of previously reported stretch-activated currents; however, SICR evoked calcium-activated chloride currents in the form of transient inward currents, suggesting a regulatory mechanism for the generation of spontaneous currents in smooth muscle. SICR was also observed in individual myocytes during stretch of intact urinary bladder smooth muscle segments. Thus, longitudinal stretch of smooth muscle cells induces Ca2+ release through gating of RYR. SICR may be an important component of the physiological response to increases in luminal pressure in smooth muscle tissues.

scholarly journals SK4 Encodes Intermediate Conductance Ca2+-Activated K+ Channels in Mouse Urinary Bladder Smooth Muscle Cells

2000 ◽  
Vol 84 (1) ◽  
pp. 97-100 ◽  
Author(s):  
Susumu Ohya ◽  
Shunichi Kimura ◽  
Mika Kitsukawa ◽  
Katsuhiko Muraki ◽  
Minoru Watanabe ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Bladder Smooth Muscle ◽  
K Channels ◽  
Urinary Bladder Smooth Muscle

scholarly journals Induction of Smooth Muscle Cell-Like Phenotype in Marrow-Derived Cells among Regenerating Urinary Bladder Smooth Muscle Cells

2005 ◽  
Vol 166 (2) ◽  
pp. 565-573 ◽  
Author(s):  
Akihiro Kanematsu ◽  
Shingo Yamamoto ◽  
Eri Iwai-Kanai ◽  
Isao Kanatani ◽  
Masaaki Imamura ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Smooth Muscle Cells ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Muscle Cells ◽  
Bladder Smooth Muscle ◽  
Urinary Bladder Smooth Muscle

scholarly journals Isolation, expansion and characterization of porcine urinary bladder smooth muscle cells for tissue engineering

2016 ◽  
Vol 18 (1) ◽  
Author(s):  
Marta Pokrywczynska ◽  
Daria Balcerczyk ◽  
Arkadiusz Jundzill ◽  
Maciej Gagat ◽  
Monika Czapiewska ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Smooth Muscle ◽  
Urinary Bladder ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Bladder Smooth Muscle ◽  
Urinary Bladder Smooth Muscle
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