Biochemical and Immunological Studies on the P400 Protein, a Protein Characteristic of the Purkinje Cell from Mouse and Rat Cerebellum

1979 ◽  
Vol 2 (6) ◽  
pp. 254-275 ◽  
Author(s):  
Katsuhiko Mikoshiba ◽  
Monique Huchet ◽  
Jean-Pierre Changeux
Keyword(s):  
Purkinje Cell ◽  
Protein A ◽  
Rat Cerebellum

Involvement of spot 35 protein, a cerebellar protein, in modulation of Purkinje cell activity of the rat cerebellum

1985 ◽  
Vol 108 (3) ◽  
pp. 309-313 ◽  
Author(s):  
Shoji Maruyama ◽  
Ge Zhang ◽  
Yoshimatsu Tamura ◽  
Tohru Yamakuni ◽  
Yasuo Takahashi
Keyword(s):  
Purkinje Cell ◽  
Protein A ◽  
Cell Activity ◽  
Rat Cerebellum

scholarly journals Autoantibody to the Rab6A/Rab6B in Autoimmune Cerebellar Ataxia Associated with Sjogren’s Syndrome

2020 ◽  
Author(s):  
Liyuan Guo ◽  
Haitao Ren ◽  
Siyuan Fan ◽  
Hongzhi Guan ◽  
Jing Wang
Keyword(s):  
Purkinje Cell ◽  
Cerebellar Ataxia ◽  
Sjögren’S Syndrome ◽  
Sjögren's Syndrome ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Hek293 Cells ◽  
Rat Cerebellum ◽  
Autoimmune Cerebellar Ataxia ◽  
Sjögren‘S Syndrome

Abstract Background To report a novel autoantibody against Purkinje cell in a patient with autoimmune cerebellar ataxia (ACA) associated to Sjogren’s syndrome (SS).Methods The Patients on one centre with cerebellar ataxia of unknown cause, who were tested positive with tissue-based indirect immunofluorescence assay (TBA) on rat cerebellum sections and negative for comprehensive anti-neural autoantibodies panel, were investigated for novel autoantibody identification. Among them, one patient with comorbid ACA and SS was qualified for further exploration. His-immunoprecipitation (HIP) combined with mass spectrometric (MS) analysis was used to identify the target antigen, which was confirmed by recombinant cell based assay (CBA) and antibody neutralization experiments. Results TBA of the patient’s serum and cerebrospinal fluid (CSF) for autoantibody testing revealed binding of IgG antibody, mainly IgG1, to Purkinje cell and granular layer of rat cerebellum. Rab6A was identified as the autoantigen by MS and Western blot, subsequently verified by CBA with HEK293 cells expressing human Rab6A/Rab6B. Furthermore, recombinant human Rab6A/Rab6B protein to neutralize the autoantibodies’ tissue reaction was performed by a parallel confirmed approach.Conclusion Autoantibody against Rab6A/Rab6B may be a novel biomarker in diagnosis of ACA, especially in patients with comorbid ACA and SS. The role of the antibody in mechanism of ACA warrants further study.

Effect of phenobarbital on Purkinje cell growth patterns in the rat cerebellum

1988 ◽  
Vol 100 (2) ◽  
pp. 354-364 ◽  
Author(s):  
R.S. Hannah ◽  
S.H. Roth ◽  
A.W. Spira
Keyword(s):  
Cell Growth ◽  
Purkinje Cell ◽  
Growth Patterns ◽  
Rat Cerebellum

Electric injury-induced Purkinje cell apoptosis in rat cerebellum: Histological and immunohistochemical study

2017 ◽  
Vol 81 ◽  
pp. 87-96 ◽  
Author(s):  
Samah Kandeel ◽  
Naema Mahmoud Elhosary ◽  
Mona Mohamed Abo El-Noor ◽  
Mohamed Balaha
Keyword(s):  
Purkinje Cell ◽  
Cell Apoptosis ◽  
Immunohistochemical Study ◽  
Rat Cerebellum ◽  
Electric Injury
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