Iron Acquisition and Expression of Iron Regulatory Proteins in the Developing Brain: Manipulation by Ethanol Exposure, Iron Deprivation and Cellular Dysfunction

1994 ◽  
Vol 16 (5-6) ◽  
pp. 233-247 ◽  
Author(s):  
James R. Connor
Keyword(s):  
Iron Acquisition ◽  
Ethanol Exposure ◽  
Developing Brain ◽  
Regulatory Proteins ◽  
Iron Regulatory Proteins ◽  
Iron Deprivation ◽  
Cellular Dysfunction

scholarly journals Bacterial effector targeting of a plant iron sensor facilitates iron acquisition and pathogen colonization

2021 ◽  
Author(s):  
Yingying Xing ◽  
Ning Xu ◽  
Deepak D Bhandari ◽  
Dmitry Lapin ◽  
Xinhua Sun ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Iron Uptake ◽  
Iron Acquisition ◽  
Iron Accumulation ◽  
Regulatory Proteins ◽  
Mineral Nutrient ◽  
Effector Protein ◽  
Iron Regulatory Proteins ◽  
Protein Levels ◽  
Pathogen Colonization

Abstract Acquisition of nutrients from different species is necessary for pathogen colonization. Iron is an essential mineral nutrient for nearly all organisms, but little is known about how pathogens manipulate plant hosts to acquire iron. Here, we report that AvrRps4, an effector protein delivered by Pseudomonas syringae bacteria to plants, interacts with and targets the plant iron sensor protein BRUTUS (BTS) to facilitate iron uptake and pathogen proliferation in Arabidopsis thaliana. Infection of rps4 and eds1 by P. syringae pv. tomato (Pst) DC3000 expressing AvrRps4 resulted in iron accumulation, especially in the plant apoplast. AvrRps4 alleviates BTS-mediated degradation of bHLH115 and ILR3(IAA-Leucine resistant 3), two iron regulatory proteins. In addition, BTS is important for accumulating immune proteins Enhanced Disease Susceptibility1 (EDS1) at both the transcriptional and protein levels upon Pst (avrRps4) infections. Our findings suggest that AvrRps4 targets BTS to facilitate iron accumulation and BTS contributes to RPS4/EDS1-mediated immune responses.

scholarly journals Expression of Iron Regulatory Proteins in Healthy and Osteoarthritic Chondrocytes

2019 ◽  
Vol 33 (S1) ◽  
Author(s):  
Brandon J Rose ◽  
Nathan F Behrens ◽  
Shelby T Harris ◽  
Sarah A Maloy ◽  
Tanner K Nelson ◽  
...  
Keyword(s):  
Regulatory Proteins ◽  
Iron Regulatory Proteins ◽  
Osteoarthritic Chondrocytes

Expression of the iron-regulatory protein haemojuvelin in retina and its regulation during cytomegalovirus infection

2009 ◽  
Vol 419 (3) ◽  
pp. 533-543 ◽  
Author(s):  
Jaya P. Gnana-Prakasam ◽  
Ming Zhang ◽  
Pamela M. Martin ◽  
Sally S. Atherton ◽  
Sylvia B. Smith ◽  
...  
Keyword(s):  
Genetic Disorder ◽  
Retinal Pigment ◽  
Primary Cultures ◽  
Regulatory Proteins ◽  
Mouse Retina ◽  
Mrna Levels ◽  
Cmv Infection ◽  
Iron Regulatory Proteins ◽  
Rpe Cells ◽  
Gene Mrna

Haemochromatosis is a genetic disorder of iron overload resulting from loss-of-function mutations in genes coding for the iron-regulatory proteins HFE [HLA-like protein involved in iron (Fe) homoeostasis], transferrin receptor 2, ferroportin, hepcidin and HJV (haemojuvelin). Expression of the first four genes coding for these proteins in retina has been established. Here we report on the expression of HJV. Since infection of retina with CMV (cytomegalovirus) causes blindness, we also investigated the expression of HJV and other iron-regulatory proteins in retina during CMV infection. HJV (HJV gene) mRNA was expressed in RPE (retinal pigment epithelium)/eyecup and neural retina in mouse. In situ hybridization and immunohistochemistry confirmed the presence of HJV mRNA and protein in RPE, outer and inner nuclear layers, and ganglion cell layer. Immunocytochemistry with cell lines and primary cell cultures showed HJV expression in RPE and Müller cells. In RPE, the expression was restricted to apical membrane. Infection of primary cultures of mouse RPE with CMV increased HJV mRNA and protein levels. Under similar conditions, HFE (HFE gene) mRNA levels were not altered, but HFE protein was decreased. Hepcidin expression was, however, not altered. These findings were demonstrable in vivo with CMV-infected mouse retina. The CMV-induced up-regulation of HJV in RPE was independent of changes in HFE because the phenomenon was also seen in HFE-null RPE cells. CMV-infected primary RPE cells showed evidence of iron accumulation and oxidative stress, as indicated by increased levels of ferritin and hydroxynonenal. The observed changes in HJV expression and iron status during CMV infection in retina may have significance in the pathophysiology of CMV retinitis

Iron homeostasis: new tales from the crypt

Blood ◽  
2000 ◽  
Vol 96 (13) ◽  
pp. 4020-4027 ◽  
Author(s):  
Cindy N. Roy ◽  
Caroline A. Enns
Keyword(s):  
Iron Uptake ◽  
Iron Transport ◽  
Iron Homeostasis ◽  
Molecular Mechanisms ◽  
Hereditary Hemochromatosis ◽  
The Body ◽  
Regulatory Proteins ◽  
Iron Regulatory Proteins ◽  
Duodenal Epithelium ◽  
Related Proteins

Abstract The enterocyte is a highly specialized cell of the duodenal epithelium that coordinates iron uptake and transport into the body. Until recently, the molecular mechanisms underlying iron absorption and iron homeostasis have remained a mystery. This review focuses on the proteins and regulatory mechanisms known to be present in the enterocyte precursor cell and in the mature enterocyte. The recent cloning of a basolateral iron transporter and investigations into its regulation provide new insights into possible mechanisms for iron transport and homeostasis. The roles of proteins such as iron regulatory proteins, the hereditary hemochromatosis protein (HFE)–transferrin receptor complex, and hephaestin in regulating this transporter and in regulating iron transport across the intestinal epithelium are discussed. A speculative, but testable, model for the maintenance of iron homeostasis, which incorporates the changes in the iron-related proteins associated with the life cycle of the enterocyte as it journeys from the crypt to the tip of the villous is proposed.

scholarly journals Iron regulatory proteins increase neuronal vulnerability to hydrogen peroxide

2008 ◽  
Vol 375 (1) ◽  
pp. 6-10 ◽  
Author(s):  
Raymond F. Regan ◽  
Zhi Li ◽  
Mai Chen ◽  
Xuefeng Zhang ◽  
Jing Chen-Roetling
Keyword(s):  
Hydrogen Peroxide ◽  
Regulatory Proteins ◽  
Iron Regulatory Proteins
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