Phospholipid Fatty Acyl Group Composition in Mitochondria, Microsomes and Plasma Membranes Isolated from Rat Astrocyte Primary Cultures: Developmental Studies

1987 ◽  
Vol 9 (4) ◽  
pp. 247-254 ◽  
Author(s):  
P. El-Achkar ◽  
A. Van Dorsselaer ◽  
L. Freysz ◽  
P. Mandel ◽  
M. Mersel
Keyword(s):  
Plasma Membranes ◽  
Group Composition ◽  
Primary Cultures ◽  
Fatty Acyl ◽  
Developmental Studies ◽  
Acyl Group Composition ◽  
Rat Astrocyte

Na-K-Cl cotransport in nystatin-treated tracheal cells: regulation by isoproterenol, apical UTP, and [Cl]i

1994 ◽  
Vol 266 (5) ◽  
pp. C1440-C1452 ◽  
Author(s):  
M. Haas ◽  
D. G. McBrayer
Keyword(s):  
Epithelial Cells ◽  
Plasma Membranes ◽  
Basolateral Membrane ◽  
Primary Cultures ◽  
Chloride Secretion ◽  
Airway Epithelial Cells ◽  
Tracheal Epithelial Cells ◽  
Cl Transport ◽  
Monovalent Ions ◽  
Stimulation Of

Chloride secretion in mammalian airway epithelia is stimulated by beta-adrenergic agonists via an adenosine 3',5'-cyclic monophosphate (cAMP)-dependent mechanism and by apical triphosphate nucleotides (ATP, UTP) via a cAMP-independent mechanism. Both types of secretagogues are known to stimulate apical Cl channels in airway cells; however, to maintain a stimulated rate of secretion, basolateral Cl influx via Na-K-Cl cotransport must be upregulated in parallel with apical Cl efflux. To examine the regulation of basolateral cotransport activity and its relationship to apical Cl efflux, we examined Cl transport in confluent primary cultures of dog tracheal epithelial cells treated with nystatin, an antibiotic that increases the permeability of plasma membranes to small monovalent ions, including Cl. By applying nystatin to the apical membrane of these cultures, apical Cl permeability could be increased to the point where transepithelial Cl transport is limited by transport across the basolateral membrane, which reflects primarily the activity of the cotransporter. In cultures of tracheal cells not treated with nystatin, transepithelial (basolateral-to-apical) 36Cl flux was increased two- to threefold by exposure to isoproterenol (5 microM, basolateral) or apical UTP (10 microM). Apical application of nystatin (400 units/ml) increased the basal level of transepithelial 36Cl flux approximately 1.5-fold and eliminated UTP stimulation of this flux, although an approximately twofold stimulation by isoproterenol persisted. Nystatin treatment also abolished UTP stimulation of saturable, basolateral [3H]bumetanide binding, a measure of functioning Na-K-Cl cotransporters in these cells; isoproterenol stimulation of binding was only mildly inhibited by nystatin treatment. Lowering intracellular Cl concentration ([Cl]i) by incubating cultures with apical media containing nystatin and reduced [Cl] (NO3 replacement) increased both basolateral-to-apical 36Cl flux and [3H]bumetanide binding in the absence of secretagogues or cell shrinkage. The results support our previous suggestion, based entirely on [3H]bumetanide binding [M. Haas, D. G. McBrayer, and J. R. Yankaskas. Am. J. Physiol. 264 (Cell. Physiol. 32): C189-C200, 1993], that UTP stimulation of basolateral Na-K-Cl cotransport in airway epithelial cells is entirely secondary to, and requires, an increase in apical Cl efflux, and further suggest that a decrease in [Cl]i may be a signal for cotransport activation in response to UTP. In addition, a cAMP-dependent cascade initiated by isoproterenol appears to directly stimulate the cotransporter.

Serum lipids and acyl group composition of alcoholic patients

Alcohol ◽  
1988 ◽  
Vol 5 (2) ◽  
pp. 153-157 ◽  
Author(s):  
Grace Y. Sun ◽  
Alan Rush ◽  
Patricia C. Chin ◽  
Christine Gorka ◽  
Suhas Lahiri ◽  
...  
Keyword(s):  
Serum Lipids ◽  
Group Composition ◽  
Acyl Group Composition
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