Influence of Basic Fibroblast Growth Factor and Astroglial Cells on the infrastructure of Developing Rat Brain Neuronal Precursors in vitro

1996 ◽  
Vol 18 (3) ◽  
pp. 210-223 ◽  
Author(s):  
Monique Miehe ◽  
Jean-François Leterrier ◽  
Jean-Christophe Deloulme ◽  
Claire Gensburger ◽  
Marie-France Knoetgen ◽  
...  
Keyword(s):  
Growth Factor ◽  
Rat Brain ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Astroglial Cells ◽  
Neuronal Precursors ◽  
Developing Rat

Localization of basic fibroblast growth factor-like immunoreactivity in the rat brain

1992 ◽  
Vol 587 (1) ◽  
pp. 49-65 ◽  
Author(s):  
A. Matsuyama ◽  
H. Iwata ◽  
N. Okumura ◽  
S. Yoshida ◽  
K. Imaizumi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Rat Brain ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth

Basic fibroblast growth factor induces retinal pigment epithelium to generate neural retina in vitro

Development ◽  
1991 ◽  
Vol 113 (2) ◽  
pp. 577-588 ◽  
Author(s):  
C. Pittack ◽  
M. Jones ◽  
T.A. Reh
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Retinal Pigment ◽  
Neural Retina ◽  
Phenotypic Switching ◽  
Surgical Removal ◽  
Fibroblast Growth ◽  
Neuronal Progenitors

During embryogenesis, the cells of the eye primordium are initially capable of giving rise to either neural retina or pigmented epithelium (PE), but become restricted to one of these potential cell fates. However, following surgical removal of the retina in embryonic chicks and larval amphibians, new neural retina is generated by the transdifferentiation, or phenotypic switching, of PE cells into neuronal progenitors. A recent study has shown that basic fibroblast growth factor (bFGF) stimulates this process in chicks in vivo. To characterize further the mechanisms by which this factor regulates the phenotype of retinal tissues, we added bFGF to enzymatically dissociated chick embryo PE. We found that bFGF stimulated proliferation and caused several morphological changes in the PE, including the loss of pigmentation; however, no transdifferentiation to neuronal phenotypes was observed. By contrast, when small sheets of PE were cultured as aggregates on a shaker device, preventing flattening and spreading on the substratum, we found that a large number of retinal progenitor cells were generated from the PE treated with bFGF. These results indicate that bFGF promotes retinal regeneration in vitro, as well as in ovo, and suggest that the ability of chick PE to undergo transdifferentiation to neuronal progenitors appears to be dependent on the physical configuration of the cells.

Basic fibroblast growth factor positively regulates hematopoietic development

Development ◽  
2000 ◽  
Vol 127 (9) ◽  
pp. 1931-1941 ◽  
Author(s):  
P. Faloon ◽  
E. Arentson ◽  
A. Kazarov ◽  
C.X. Deng ◽  
C. Porcher ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Es Cells ◽  
Embryonic Stem ◽  
Growth Factor Receptor ◽  
Fibroblast Growth ◽  
Hematopoietic Lineage ◽  
Mesoderm Inducing Factors

Recently identified BLast Colony Forming Cells (BL-CFCs) from in vitro differentiated embryonic stem (ES) cells represent the common progenitor of hematopoietic and endothelial cells, the hemangioblast. Access to this initial cell population committed to the hematopoietic lineage provides a unique opportunity to characterize hematopoietic commitment events. Here, we show that BL-CFC expresses the receptor tyrosine kinase, Flk1, and thus we took advantage of the BL-CFC assay, as well as fluorescent activated cell sorter (FACS) analysis for Flk1(+) cells to determine quantitatively if mesoderm-inducing factors promote hematopoietic lineage development. Moreover, we have analyzed ES lines carrying targeted mutations for fibroblast growth factor receptor-1 (fgfr1), a receptor for basic fibroblast growth factor (bFGF), as well as scl, a transcription factor, for their potential to generate BL-CFCs and Flk1(+) cells, to further define events leading to hemangioblast development. Our data suggest that bFGF-mediated signaling is critical for the proliferation of the hemangioblast and that cells expressing both Flk1 and SCL may represent the hemangioblast.

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