Suppression of Renal Tubulointerstitial Fibrosis by Small Interfering RNA Targeting Heat Shock Protein 47

2007 ◽  
Vol 28 (1) ◽  
pp. 34-46 ◽  
Author(s):  
Zhiyin Xia ◽  
Katsushige Abe ◽  
Akira Furusu ◽  
Masanobu Miyazaki ◽  
Yoko Obata ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Small Interfering Rna ◽  
Tubulointerstitial Fibrosis ◽  
Heat Shock Protein 47 ◽  
Renal Tubulointerstitial Fibrosis ◽  
Rna Targeting ◽  
Interfering Rna

scholarly journals Synthetic small interfering RNA targeting heat shock protein 105 induces apoptosis of various cancer cells both in vitro and in vivo

2006 ◽  
Vol 97 (7) ◽  
pp. 623-632 ◽  
Author(s):  
Seiji Hosaka ◽  
Tetsuya Nakatsura ◽  
Hirotake Tsukamoto ◽  
Takumi Hatayama ◽  
Hideo Baba ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Cancer Cells ◽  
Small Interfering Rna ◽  
Rna Targeting ◽  
Interfering Rna

scholarly journals Laser-Triggered Small Interfering RNA Releasing Gold Nanoshells against Heat Shock Protein for Sensitized Photothermal Therapy

2016 ◽  
Vol 4 (2) ◽  
pp. 1600327 ◽  
Author(s):  
Zhaohui Wang ◽  
Siwen Li ◽  
Min Zhang ◽  
Yi Ma ◽  
Yuxi Liu ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Photothermal Therapy ◽  
Small Interfering Rna ◽  
Gold Nanoshells ◽  
Interfering Rna

scholarly journals Silencing Heat Shock Protein 47 (HSP47) in Fibrogenic Precision-Cut Lung Slices: A Surprising Lack of Effects on Fibrogenesis?

2021 ◽  
Vol 8 ◽  
Author(s):  
Mitchel J. R. Ruigrok ◽  
Khaled E. M. El Amasi ◽  
Diana J. Leeming ◽  
Jannie M. B. Sand ◽  
Henderik W. Frijlink ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Transforming Growth Factor ◽  
Therapeutic Potential ◽  
Scar Tissue ◽  
Transforming Growth Factor Β1 ◽  
Heat Shock Protein 47 ◽  
Collagen Secretion ◽  
Precision Cut Lung Slices ◽  
Interfering Rna

Idiopathic pulmonary fibrosis (IPF) is a chronic disease that is characterized by the excessive deposition of scar tissue in the lungs. As currently available treatments are unable to restore lung function in patients, there is an urgent medical need for more effective drugs. Developing such drugs, however, is challenging because IPF has a complex pathogenesis. Emerging evidence indicates that heat shock protein 47 (HSP47), which is encoded by the gene Serpinh1, may be a suitable therapeutic target as it is required for collagen synthesis. Pharmacological inhibition or knockdown of HSP47 could therefore be a promising approach to treat fibrosis. The objective of this study was to assess the therapeutic potential of Serpinh1-targeting small interfering RNA (siRNA) in fibrogenic precision-cut lung slices prepared from murine tissue. To enhance fibrogenesis, slices were cultured for up to 144 h with transforming growth factor β1. Self-deliverable siRNA was used to knockdown mRNA and protein expression, without affecting the viability and morphology of slices. After silencing HSP47, only the secretion of fibronectin was reduced while other aspects of fibrogenesis remained unaffected (e.g., myofibroblast differentiation as well as collagen secretion and deposition). These observations are surprising as others have shown that Serpinh1-targeting siRNA suppressed collagen deposition in animals. Further studies are therefore warranted to elucidate downstream effects on fibrosis upon silencing HSP47.

scholarly journals Pseudogenes

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Yusuf Tutar
Keyword(s):  
Gene Regulation ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Tumor Suppressors ◽  
Small Interfering Rna ◽  
Current Knowledge ◽  
Heat Shock Protein 90 ◽  
A Genome ◽  
Interfering Rna ◽  
Mirna Concentration

Pseudogenes are ubiquitous and abundant in genomes. Pseudogenes were once called “genomic fossils” and treated as “junk DNA” several years. Nevertheless, it has been recognized that some pseudogenes play essential roles in gene regulation of their parent genes, and many pseudogenes are transcribed into RNA. Pseudogene transcripts may also form small interfering RNA or decrease cellular miRNA concentration. Thus, pseudogenes regulate tumor suppressors and oncogenes. Their essential functions draw the attention of our research group in my current work on heat shock protein 90: a chaperone of oncogenes. The paper reviews our current knowledge on pseudogenes and evaluates preliminary results of the chaperone data. Current efforts to understand pseudogenes interactions help to understand the functions of a genome.

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