Dendritic Cells Reconstituted with a Human Heparanase Gene Induce Potent Cytotoxic T-Cell Responses against Gastric Tumor Cells in vitro

Tumor Biology ◽  
2007 ◽  
Vol 28 (4) ◽  
pp. 238-246 ◽  
Author(s):  
Yong-Guo Cai ◽  
Dian-Chun Fang ◽  
Ling Chen ◽  
Xu-Dong Tang ◽  
Ting Chen ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
Tumor Cells ◽  
T Cell Responses ◽  
Gastric Tumor ◽  
Cytotoxic T Cell ◽  
Cell Responses ◽  
Cytotoxic T Cell Responses

Potency of Mature CD40L RNA Electroporated Dendritic Cells Correlates With IL-12 Secretion by Tracking Multifunctional CD8+/CD28+ Cytotoxic T-cell Responses In Vitro

2011 ◽  
Vol 34 (1) ◽  
pp. 45-57 ◽  
Author(s):  
Mark A. DeBenedette ◽  
David M. Calderhead ◽  
Irina Y. Tcherepanova ◽  
Charles A. Nicolette ◽  
Don G. Healey
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
T Cell Responses ◽  
Cytotoxic T Cell ◽  
Cell Responses ◽  
Cytotoxic T Cell Responses

scholarly journals Heat Shock Treatment of Tumor Lysate-Pulsed Dendritic Cells Enhances Their Capacity to Elicit Antitumor T Cell Responses against Medullary Thyroid Carcinoma

2006 ◽  
Vol 91 (11) ◽  
pp. 4571-4577 ◽  
Author(s):  
Thomas Bachleitner-Hofmann ◽  
Michaela Strohschneider ◽  
Peter Krieger ◽  
Monika Sachet ◽  
Peter Dubsky ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Heat Shock ◽  
Tumor Cells ◽  
T Cell Responses ◽  
Cytotoxic T Cell ◽  
Tumor Lysate ◽  
Cell Responses ◽  
Cytotoxic T Cell Responses

Abstract Background: In vitro and in vivo studies have shown that dendritic cells (DCs) can stimulate antitumor T cell responses against medullary thyroid carcinoma (MTC). However, despite promising results in selected cases, the clinical efficacy of DC immunotherapy in patients with MTC has been limited. Recently, it has been demonstrated in mice that heat shock enhances the capacity of bone-marrow-derived DCs to stimulate antigen-specific T cells. The aim of our investigations was to evaluate whether heat shock also increases the capacity of human monocyte-derived DCs to stimulate antitumor T cell responses against MTC tumor cells. Methods: DCs from six patients with metastatic MTC were pulsed with tumor lysate derived from allogeneic MTC tumor cells and were heat shocked for 12 h at 40 C or kept at 37 C. Thereafter, the DCs were matured and cocultured with T cells. Finally, the cytotoxic activity of T cells against MTC tumor cells was measured in vitro. Results: In all patient samples, cytotoxic T cell responses against MTC tumor cells could be induced. Notably, heat-shocked DCs were more potent stimulators of cytotoxic T cell responses than control DCs, with T cells stimulated with heat-shocked DCs displaying a significantly increased cytotoxic activity against MTC tumor cells as compared with T cells stimulated with control DCs. In none of the experiments was a cytotoxic T cell response against unrelated pancreatic tumor cells (PANC-1) observed, using both control and heat-shocked DCs. Conclusions: Our study shows that heat-shocking DCs may be a valuable strategy to increase the immunostimulatory capacity of DCs used for immunotherapy of MTC.

Cytotoxic T Cell Responses against Mesothelioma by Apoptotic Cell-pulsed Dendritic Cells

2004 ◽  
Vol 169 (12) ◽  
pp. 1322-1330 ◽  
Author(s):  
Frédéric Ebstein ◽  
Carole Sapede ◽  
Pierre-Joseph Royer ◽  
Marie Marcq ◽  
Catherine Ligeza-Poisson ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
Apoptotic Cell ◽  
T Cell Responses ◽  
Cytotoxic T Cell ◽  
Cell Responses ◽  
Cytotoxic T Cell Responses

Dendritic cells overexpressing CD95 (Fas) ligand elicit vigorous allospecific T-cell responses in vivo

Blood ◽  
2003 ◽  
Vol 101 (4) ◽  
pp. 1469-1476 ◽  
Author(s):  
Sofia Buonocore ◽  
Frédéric Paulart ◽  
Alain Le Moine ◽  
Michel Braun ◽  
Isabelle Salmon ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
Fas Ligand ◽  
T Cell Responses ◽  
Genetically Engineered ◽  
Peripheral Tolerance ◽  
Cytotoxic T Cell ◽  
Cell Responses

Dendritic cells (DCs) genetically engineered to overexpress CD95 (Fas) ligand (CD95L-DC) were proposed as tools to induce peripheral tolerance to alloantigens. Herein, we observed that CD95L-DC obtained after retroviral gene transfer in bone marrow (BM) precursors derived from CD95-deficient (lpr/lpr) mice elicit much stronger allospecific type 1 helper T-cell and cytotoxic T-cell activities than control DCs upon injection in vivo, although they induce lower T-cell responses in vitro. Indeed, a single injection of CD95L-DC prepared from C57BL/6 mice was sufficient to prime bm13 recipients for acute rejection of C57BL/6 skin allografts that were otherwise tolerated in the context of this single weak major histocompatibility complex (MHC) class I incompatibility. Massive neutrophil infiltrates depending on interleukin (IL)–1 signaling were observed at sites of CD95L-DC injection. Experiments in IL-1 receptor–deficient mice or in animals injected with depleting anti-Gr1 monoclonal antibody (mAb) established that neutrophil recruitment is required for the development of vigorous T-cell responses after injection of CD95L-DC in vivo.

Long-lived fusions of human haematological tumour cells and B-lymphoblastoid cells induce tumour antigen-specific cytotoxic T-cell responses in vitro

Immunobiology ◽  
2012 ◽  
Vol 217 (7) ◽  
pp. 719-729 ◽  
Author(s):  
Yehia S. Mohamed ◽  
Debbie Dunnion ◽  
Iryna Teobald ◽  
Renata Walewska ◽  
Michael J. Browning
Keyword(s):  
T Cell ◽  
T Cell Responses ◽  
Tumour Cells ◽  
Tumour Antigen ◽  
Cytotoxic T Cell ◽  
Lymphoblastoid Cells ◽  
Cell Responses ◽  
Cytotoxic T Cell Responses
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