Detection of Active Fraction of Glycogen Synthase Kinase 3β in Cancer Cells by Nonradioisotopic in vitro Kinase Assay

Oncology ◽  
2006 ◽  
Vol 71 (3-4) ◽  
pp. 297-305 ◽  
Author(s):  
Wei Mai ◽  
Katsuyoshi Miyashita ◽  
Abbas Shakoori ◽  
Bin Zhang ◽  
Zhi Wei Yu ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Glycogen Synthase ◽  
Glycogen Synthase Kinase ◽  
Kinase Assay ◽  
Glycogen Synthase Kinase 3Β ◽  
Active Fraction ◽  
In Vitro Kinase Assay

Microtubule-associated protein 1B phosphorylation by glycogen synthase kinase 3β is induced during PC12 cell differentiation

2001 ◽  
Vol 114 (23) ◽  
pp. 4273-4284 ◽  
Author(s):  
Robert G. Goold ◽  
Phillip R. Gordon-Weeks
Keyword(s):  
Cell Differentiation ◽  
Pc12 Cell ◽  
Glycogen Synthase ◽  
Axon Outgrowth ◽  
Glycogen Synthase Kinase ◽  
Glycogen Synthase Kinase 3Β ◽  
Cell Extracts ◽  
Neurite Formation ◽  
Reduced Mobility

In recent studies we have demonstrated that glycogen synthase kinase 3β (GSK3β) and its substrate microtubule-associated protein 1B (MAP1B) regulate the microtubule cytoskeleton during axon outgrowth. To further examine the role GSK3β plays in axon outgrowth we investigated the expression of GSK3β and its activity towards MAP1B during nerve growth factor (NGF)-stimulated PC12 cell differentiation. Levels of GSK3β expression increase relatively little during the course of differentiation. However, the expression of a novel GSK3β isoform characterised by a reduced mobility on SDS gels is induced by NGF. Expression of this isoform and the GSK3β-phosphorylated isoform of MAP1B (MAP1B-P) are induced in parallel in response to NGF. This increase lags behind initial neurite formation and the expression of MAP1B in these cells by about two days and coincides with a period when the majority of cells are extending existing neurites. MAP1B and GSK3β are expressed throughout the PC12 cell but MAP1B-P expression is restricted to the growth cones and neurites. Consistent with these observations, we find that neurite extension is more sensitive to the GSK3 inhibitor Li+ than neurite formation and that this correlates with an inhibition of MAP1B phosphorylation. Additionally, GSK3β from PC12 cells not exposed to NGF can not phosphorylate MAP1B in vitro. However, a soluble factor in differentiated PC12 cell extracts depleted of GSK3β can activate MAP1B phosphorylation from undifferentiated cell extracts otherwise devoid of kinase activity. These experiments provide evidence for an NGF-mediated regulation of MAP1B phosphorylation in growing neurites by the induction of a novel isoform of GSK3β.

scholarly journals Genetic or pharmacological superoxide-hydrogen peroxide imbalances modulate the in vitro effects of lithium on glycogen synthase kinase-3β

Gene ◽  
2018 ◽  
Vol 655 ◽  
pp. 48-55 ◽  
Author(s):  
Fernanda Barbisan ◽  
Verônica Farina Azzolin ◽  
Gustavo Cardenas Monteiro ◽  
Cibele F. Teixeira ◽  
Moisés Henrique Mastella ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Glycogen Synthase ◽  
Glycogen Synthase Kinase ◽  
Glycogen Synthase Kinase 3Β ◽  
In Vitro Effects

scholarly journals Expression of Cyclooxygenase-2 Is Regulated by Glycogen Synthase Kinase-3β in Gastric Cancer Cells

2005 ◽  
Vol 281 (8) ◽  
pp. 4564-4569 ◽  
Author(s):  
Alexandra Thiel ◽  
Mira Heinonen ◽  
Johanna Rintahaka ◽  
Tuija Hallikainen ◽  
Annabrita Hemmes ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Glycogen Synthase ◽  
Cyclooxygenase 2 ◽  
Glycogen Synthase Kinase ◽  
Glycogen Synthase Kinase 3Β ◽  
Gastric Cancer Cells
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