Stereoencephalotomy and Control of Skeletal Muscle Tone

1990 ◽  
Vol 54 (1-8) ◽  
pp. 136-139 ◽  
Author(s):  
A. Struppler ◽  
T. Plant ◽  
M.T. Jahnke ◽  
H. Riescher
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tone ◽  
And Control

scholarly journals In vivo uptake of [14C]leucine by skeletal muscle ribosomes after injury in rats fed two levels of protein

1969 ◽  
Vol 23 (2) ◽  
pp. 271-280 ◽  
Author(s):  
V. R. Young ◽  
P. C. Huang
Keyword(s):  
Skeletal Muscle ◽  
Specific Activity ◽  
Male Rats ◽  
Thigh Muscle ◽  
Left Femur ◽  
The Right ◽  
And Control ◽  
The Relationship ◽  
In Vivo Uptake

1. After 14 days on a diet containing 5 or 25% casein male rats received a fracture of the left femur. Four hours before they were killed the injured and control rats were injected with [1-14C]leucine; the incorporation of radioactivity into an isolated fraction of skeletal muscle ribosomes was studied 6, 12, 24, 48, 72, 96 and 228 h after injury.2. The incorporation of [14C]leucine into the ribosome fraction in right thigh muscles dropped to 40% of control values 72 h after fracture in well-nourished rats and after 96 h with diets containing 5 or 25%, casein.3. The specific activity of the trichloroacetic acid-soluble fraction of muscle from injured rats was equal to or higher than that of the controls during the first 72 h but lower at 96 h.4. These results suggest that a reduced incorporation of amino acids by ribosomes from the right thigh muscle occurred on day 3 after fracture in the group receiving 25% casein but not in the group receiving 5% casein.5. Muscle RNA and DNA concentrations were not affected by the injury.6. The relationship between these findings and the loss of muscle N after injury is discussed.

scholarly journals The effects of simulated microgravity on skeletal muscle of Japanese quail: transmission electron microscopic study

2011 ◽  
Vol 80 (1) ◽  
pp. 119-124 ◽  
Author(s):  
Katarína Holovská ◽  
Viera Almášiová ◽  
Viera Cigánková ◽  
Peter Škrobánek
Keyword(s):  
Skeletal Muscle ◽  
Japanese Quail ◽  
Structural Changes ◽  
Simulated Microgravity ◽  
Negative Impact ◽  
Electron Microscopic ◽  
Giant Mitochondria ◽  
Transmission Electron ◽  
Muscle Tissues ◽  
And Control

The aim of the present study was to investigate the effects of simulated microgravity (hypodynamia) on the structure of the skeletal muscle (m. gastrocnemius) in developing Japanese quail by transmission electron microscopy. Samples of muscle tissues from experimental (n = 28) and control (n = 28) birds were collected at day 7, 14, 28, 42 and 56 of age. The structure of m. gastrocnenmius was changed depending on hypodynamia length. The first extensive structural changes were found on day 14 of age. The mitochondria were enlarged and the spaces between the myofibrils were slightly extended compared to control. The sarcomeres were irregular and lipid droplets occurred in the sarcoplasm. Further developmental changes occurred on day 28 of age. Mitochondria fused into the giant mitochondria which frequently exceeded the length of one sarcomere. Moreover, at 42 days of age, beside the above mentioned changes, sarcoplasmic reticulum was dilated and the number of mitochondrial cristae was reduced. However, the structure of m. gastrocnemius on day 56 was less damaged compared to the damage observed on day 42 of age. Presented results indicate that the continuous stay of male Japanese quail under simulated microgravity has a negative impact on the structure of m. gastrocnemius, but also the ability of muscle tissue to cope with these specific conditions.

scholarly journals Intermuscular Fat Content in Young Chinese Men With Newly Diagnosed Type 2 Diabetes: Based on MR mDIXON-Quant Quantitative Technique

2021 ◽  
Vol 12 ◽  
Author(s):  
Fuyao Yu ◽  
Bing He ◽  
Li Chen ◽  
Fengzhe Wang ◽  
Haidong Zhu ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Skeletal Muscle ◽  
Fat Content ◽  
Control Group ◽  
Diabetic Patients ◽  
Diabetes Group ◽  
Control Subjects ◽  
Intermuscular Fat ◽  
And Control

ObjectiveSkeletal muscle fat content is one of the important contributors to insulin resistance (IR), but its diagnostic value remains unknown, especially in the Chinese population. Therefore, we aimed to analyze differences in skeletal muscle fat content and various functional MRI parameters between diabetic patients and control subjects to evaluate the early indicators of diabetes. In addition, we aimed to investigate the associations among skeletal muscle fat content, magnetic resonance parameters of skeletal muscle function and IR in type 2 diabetic patients and control subjects.MethodsWe enrolled 12 patients (age:29-38 years, BMI: 25-28 kg/m2) who were newly diagnosed with type 2 diabetes (intravenous plasma glucose concentration≥11.1mmol/l or fasting blood glucose concentration≥7.0mmol/l) together with 12 control subjects as the control group (age: 26-33 years, BMI: 21-28 kg/m2). Fasting blood samples were collected for the measurement of glucose, insulin, 2-hour postprandial blood glucose (PBG2h), and glycated hemoglobin (HbAlc). The magnetic resonance scan of the lower extremity and abdomen was performed, which can evaluate visceral fat content as well as skeletal muscle metabolism and function through transverse relaxation times (T2), fraction anisotropy (FA) and apparent diffusion coefficient (ADC) values.ResultsWe found a significant difference in intermuscular fat (IMAT) between the diabetes group and the control group (p<0.05), the ratio of IMAT in thigh muscles of diabetes group was higher than that of control group. In the entire cohort, IMAT was positively correlated with HOMA-IR, HbAlc, T2, and FA, and the T2 value was correlated with HOMA-IR, PBG2h and HbAlc (p<0.05). There were also significant differences in T2 and FA values between the diabetes group and the control group (p<0.05). According to the ROC, assuming 8.85% of IMAT as the cutoff value, the sensitivity and specificity of IMAT were 100% and 83.3%, respectively. Assuming 39.25ms as the cutoff value, the sensitivity and specificity of T2 value were 66.7% and 91.7%, respectively. All the statistical analyses were adjusted for age, BMI and visceral fat content.ConclusionDeposition of IMAT in skeletal muscles seems to be an important determinant for IR in type 2 diabetes. The skeletal muscle IMAT value greater than 8.85% and the T2 value greater than 39.25ms are suggestive of IR.

scholarly journals PO-055 Changes of trace elements in skeletal muscle and serum of rats after exercise-induced injury

2018 ◽  
Vol 1 (3) ◽  
Author(s):  
Jingyun Liu ◽  
Qun Zuo
Keyword(s):  
Skeletal Muscle ◽  
Trace Elements ◽  
Nitric Acid ◽  
Vena Cava ◽  
The Body ◽  
Emission Spectrometry ◽  
Control Group ◽  
Exercise Induced ◽  
And Control ◽  
Cu And Zn

Objective This study is to investigate the changes of trace elements (Cu, Fe, Zn, Se, Mg) in serum and skeletal muscle of rats after skeletal muscle injury induced by downhill running, and to find out the change regularity of trace elements in the body after exercise injury. To provide experimental basis for how to use trace elements supplements reasonably. Methods Fifty-four healthy male Sprague-Dawley rats aged 8 weeks were randomly divided into two groups: control group (C, N=6) and exercise group (E, N=48, include: 0 h group, 6 h group, 12 h group, 24 h group, 48 h group, 72 h group, 1- week group and 2- week group). The rats in exercise groups run down a 16°incline at 16m/min for 90 minutes. At the end of the exercise, the rats were killed at 0 h, 6 h, 12 h, 24 h, 48 h, 72 h, 1 week and 2 weeks, respectively. The serum was got from the inferior vena cava blood and diluted by 1% nitric acid. The muscle was got from the right side of the rat's sural which were digested by concentrated nitric acid and 30% hydrogen peroxide in 75℃water bath for 20mins. The content of trace elements in muscle and serum were measured by inductively coupled plasma atomic emission spectrometry (ICP-MS). All the data are analyzed and processed by SPSS22.0 statistical software. Results (1) The contents of trace elements in serum showed: Cu, Zn, Mg, Se decreased immediately after exercise, but the Cu still increased to reach a peak at 24h after decreasing, and after 2 weeks the content of Cu was slightly lower than pre-exercise level. However, the content of Zn did not elevate again, it continued declined to the lowest at 24h which was significantly lower than control group (P < 0.05). And after 2 weeks, Zn did not return to the pre-exercise level. The changes of Mg, Se in serum was not statistically significant. There is no difference between 0h and control groups in content of Fe, after that Fe decreased continually and appeared the least value at 24h, the differences between immediate group and control group were statistically significant (P < 0.05). Fe returned to the pre-exercise level after 2 weeks. (2) The contents of trace elements in muscle showed: Most of trace elements increased to the maximum level at 6 h, after that Mg, Fe, Cu decreased to the lowest value at 72 h which were significant lower than 0h group or 6h group (P < 0. 05). ALL the trace elements were lower than pre-exercise level. There was no statistical difference in the content of Se in muscle. Conclusions (1) The different changes of trace elements in skeletal muscle and serum after exercise injury may be due to the redistribution of trace elements caused by the body adaptability. (2) The most obviously changes of trace element in serum and muscle are Cu and Zn. Both of them did not return to the pre-exercise level after 2 weeks, it suggests that the supplement include Cu and Zn may play an important role in recovering after exercise-induced injury.

Nur77 Increases Glucose Transport in Mouse Skeletal Muscle Cells by Activating p38MAPK Under Lipotoxicity

2020 ◽  
Vol 10 (12) ◽  
pp. 1832-1836
Author(s):  
Yan Xu ◽  
Ming Zhang ◽  
Jianying Wang ◽  
Tianwei Gao
Keyword(s):  
Skeletal Muscle ◽  
Signaling Pathway ◽  
Glucose Transport ◽  
Cell Morphology ◽  
Glucose Concentration ◽  
Cell Activity ◽  
Muscle Cells ◽  
Control Group ◽  
P38mapk Signaling ◽  
And Control

Diabetes Mellitus (DM) is a common disease in clinic and belongs to a metabolic disease. Related studies have shown that Nur77 regulates sugar metabolism in a variety of tissue cells. Nur77 (orphan nuclear receptor) is a transcription factor and involves in glucose metabolism are different in skeletal muscle cells via p38MAPK signaling pathway. In the study, an IR model was to explore the mechanism of skeletal muscle IR. L6 myocytes were cultured and control, Nur77 knockout and Nur77 overexpression group was set followed by analysis of L6 muscle cell morphology, activity by MTT assay, glucose concentration by glucose oxidase-peroxidase (GOD-POD) p-p38MAPK and skeletal muscle glucose transporter-4 (GLUT4) level by western blot. After 8 to 12 hours of culture, the boundaries between cells were unknown with irregular order and no muscle-shaped structure. The cell morphology was mainly spindle-shaped and triangular. After 2 to 3 days, cell arrangement was regular and morphology was mainly spindle-shaped. The cell activity and glucose concentration in control group at different time points showed no differences which were significantly different in knockout and overexpression group with significantly higher cell activity and glucose concentration for overexpression group than knockout and control group (P < 0.05). In addition, overexpression group also showed significantly increased expression of p-p38MAPK4 and GLUT4. Nur77 can increase glucose transport under lipotoxic state by activating p38MAPK signaling pathway and increasing the protein expression of p-p38MAPK and GLUT4 in muscle cells.

scholarly journals EFFECTIVENESS OF VAGINAL CONE THERAPY ON URINARY INCONTINENCE AMONG WOMEN IN SAVEETHA MEDICAL COLLEGE HOSPITAL, THANDALAM, CHENNAI

2019 ◽  
pp. 133-136
Author(s):  
JAGADEESWARI J ◽  
KALABARATHI S ◽  
MANGALAGOWRI P
Keyword(s):  
Urinary Incontinence ◽  
Muscle Tone ◽  
Sampling Technique ◽  
Structured Interview ◽  
Moderate Level ◽  
Control Group ◽  
College Hospital ◽  
Study Results ◽  
And Control ◽  
Experimental Group

 Objectives: The present aims to assess the level of urinary incontinence in experimental and control group, to assess the effectiveness of vaginal cone therapy on urinary incontinence in experimental group and control group and to associate the post-test level of urinary incontinence with the selected demographic variables among women in the experimental group. Methods: A quantitative quasi-experimental research design was conducted among 60 women with urinary incontinence. Convenience sampling technique was used. Semi-structured interview method was used to collect the demographical data and level of urinary incontinence among women was assessed by urinary incontinence diagnosis questionnaire. Results: Among 60 samples in experimental group 26 women had a moderate level of urinary incontinence, and 4 women had slight urinary incontinence whereas in control group 24 women had moderate level of urinary incontinence and 6 women had slight urinary incontinence. The study results show significant improvement in level of urinary incontinence among experimental group than the control group after the intervention at the level of p<0.05. This reveals that vaginal cone therapy is highly significant in the experimental group because pelvic muscle strength had improved and allows the patient to increase the physiological consciousness and promotes the muscle tone after the use of a vaginal cone. Conclusion: This study proves that vaginal cone therapy is the effective non-pharmacological method, cost-effective method and had no side effects which can be used to treat female urinary incontinence.

scholarly journals Increased pyruvate dehydrogenase activity in skeletal muscle of growth-restricted ovine fetuses

2019 ◽  
Vol 317 (4) ◽  
pp. R513-R520 ◽  
Author(s):  
Alexander L. Pendleton ◽  
Laurel R. Humphreys ◽  
Melissa A. Davis ◽  
Leticia E. Camacho ◽  
Miranda J. Anderson ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Pyruvate Dehydrogenase ◽  
Intrauterine Growth Restriction ◽  
Citrate Synthase ◽  
Pyruvate Dehydrogenase Kinase ◽  
Semitendinosus Muscle ◽  
Fetal Sheep ◽  
Pyruvate Dehydrogenase Phosphatase ◽  
And Control ◽  
Fetal Muscle

Fetal sheep with placental insufficiency-induced intrauterine growth restriction (IUGR) have lower fractional rates of glucose oxidation and greater gluconeogenesis, indicating lactate shuttling between skeletal muscle and liver. Suppression of pyruvate dehydrogenase ( PDH) activity was proposed because of greater pyruvate dehydrogenase kinase (PDK) 4 and PDK1 mRNA concentrations in IUGR muscle. Although PDK1 and PDK4 inhibit PDH activity to reduce pyruvate metabolism, PDH protein concentrations and activity have not been examined in skeletal muscle from IUGR fetuses. Therefore, we evaluated the protein concentrations and activity of PDH and the kinases and phosphatases that regulate PDH phosphorylation status in the semitendinosus muscle from placenta insufficiency-induced IUGR sheep fetuses and control fetuses. Immunoblots were performed for PDH, phosphorylated PDH (E1α), PDK1, PDK4, and pyruvate dehydrogenase phosphatase 1 and 2 (PDP1 and PDP2, respectively). Additionally, the PDH, lactate dehydrogenase (LDH), and citrate synthase (CS) enzymatic activities were measured. Phosphorylated PDH concentrations were 28% lower (P < 0.01) and PDH activity was 67% greater (P < 0.01) in IUGR fetal muscle compared with control. PDK1, PDK4, PDP1, PDP2, and PDH concentrations were not different between groups. CS and LDH activities were also unaffected. Contrary to the previous speculation, PDH activity was greater in skeletal muscle from IUGR fetuses, which parallels lower phosphorylated PDH. Therefore, greater expression of PDK1 and PDK4 mRNA did not translate to greater PDK1 or PDK4 protein concentrations or inhibition of PDH as proposed. Instead, these findings show greater PDH activity in IUGR fetal muscle, which indicates that alternative regulatory mechanisms are responsible for lower pyruvate catabolism.

THE INFLUENCE OF HEMORRHAGE ON SKELETAL MUSCLE TONE

1942 ◽  
Vol 137 (2) ◽  
pp. 251-255 ◽  
Author(s):  
W. F. Kiely ◽  
S. L. Hamilton ◽  
E. Gellhorn
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tone
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