Reliable Generation of Stable High Titer Producer Cell Lines for Gene Therapy

Intervirology ◽  
2007 ◽  
Vol 50 (3) ◽  
pp. 197-203 ◽  
Author(s):  
Ina Rattmann ◽  
Veronika Kleff ◽  
Anja Feldmann ◽  
Carsten Ludwig ◽  
Ursula Regina Sorg ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Cell Lines ◽  
High Titer ◽  
Producer Cell

scholarly journals High-Throughput Selection of Retrovirus Producer Cell Lines Leads to Markedly Improved Efficiency of Germ Line-Transmissible Insertions in Zebra Fish

2002 ◽  
Vol 76 (5) ◽  
pp. 2192-2198 ◽  
Author(s):  
Wenbiao Chen ◽  
Shawn Burgess ◽  
Greg Golling ◽  
Adam Amsterdam ◽  
Nancy Hopkins
Keyword(s):  
Cell Lines ◽  
Large Scale ◽  
Insertional Mutagenesis ◽  
Germ Line ◽  
High Titer ◽  
Retroviral Vectors ◽  
Embryonic Cells ◽  
Zebra Fish ◽  
Glycoprotein G ◽  
Producer Cell

ABSTRACT Vesicular stomatitis virus glycoprotein G-pseudotyped mouse retroviral vectors have been used as mutagens for a large-scale insertional mutagenesis screen in the zebra fish. To reproducibly generate high-titer virus stocks, we devised a method for rapidly selecting cell lines that can yield high-titer viruses and isolated a producer cell line that yields virus at a high titer on zebra fish embryos. Virus produced from this line, designated GT virus, is nontoxic following injection of zebra fish blastulae and efficiently infects embryonic cells that give rise to the future germ line. Using GT virus preparations we generated roughly 500,000 germ line-transmissible proviral insertions in a population of 25,000 founder fish in about 2 months. The GT virus contains a gene trap, and trap events can be detected in the offspring of almost every founder fish. We discuss potential applications of this highly efficient method for generating germ line-transmissible insertions in a vertebrate

scholarly journals Ross River Virus Glycoprotein-Pseudotyped Retroviruses and Stable Cell Lines for Their Production

2001 ◽  
Vol 75 (6) ◽  
pp. 2653-2659 ◽  
Author(s):  
C. Matthew Sharkey ◽  
Cynthia L. North ◽  
Richard J. Kuhn ◽  
David Avram Sanders
Keyword(s):  
Gene Therapy ◽  
Gene Transfer ◽  
Cell Lines ◽  
Viral Entry ◽  
High Titer ◽  
Leukemia Virus ◽  
Ross River Virus ◽  
Stable Gene ◽  
Viral Glycoprotein ◽  
Stable Cell Lines

ABSTRACT Pseudotyped retroviruses have important applications as vectors for gene transfer and gene therapy and as tools for the study of viral glycoprotein function. Recombinant Moloney murine leukemia virus (Mo-MuLV)-based retrovirus particles efficiently incorporate the glycoproteins of the alphavirus Ross River virus (RRV) and utilize them for entry into cells. Stable cell lines that produce the RRV glycoprotein-pseudotyped retroviruses for prolonged periods of time have been constructed. The pseudotyped viruses have a broadened host range, can be concentrated to high titer, and mediate stable transduction of genes into cells. The RRV glycoprotein-pseudotyped retroviruses and the cells that produce them have been employed to demonstrate that RRV glycoprotein-mediated viral entry occurs through endocytosis and that membrane fusion requires acidic pH. Alphavirus glycoprotein-pseudotyped retroviruses have significant advantages as reagents for the study of the biochemistry and prevention of alphavirus entry and as preferred vectors for stable gene transfer and gene therapy protocols.

scholarly journals Generation of Retroviral Packaging and Producer Cell Lines for Large-Scale Vector Production and Clinical Application: Improved Safety and High Titer

2000 ◽  
Vol 2 (3) ◽  
pp. 262-275 ◽  
Author(s):  
Philip L. Sheridan ◽  
Mordechai Bodner ◽  
Andrea Lynn ◽  
Trung K. Phuong ◽  
Nicholas J. DePolo ◽  
...  
Keyword(s):  
Cell Lines ◽  
Clinical Application ◽  
Large Scale ◽  
High Titer ◽  
Producer Cell

scholarly journals Development of high-titer retroviral producer cell lines by using Cre-mediated recombination.

1997 ◽  
Vol 71 (10) ◽  
pp. 7820-7826 ◽  
Author(s):  
E F Vanin ◽  
L Cerruti ◽  
N Tran ◽  
G Grosveld ◽  
J M Cunningham ◽  
...  
Keyword(s):  
Cell Lines ◽  
High Titer ◽  
Producer Cell

scholarly journals Novel approaches to render stable producer cell lines viable for the commercial manufacturing of rAAV-based gene therapy vectors

2013 ◽  
Vol 7 (S6) ◽  
Author(s):  
Verena V Emmerling ◽  
Karlheinz Holzmann ◽  
Karin Lanz ◽  
Stefan Kochanek ◽  
Markus Hörer
Keyword(s):  
Gene Therapy ◽  
Cell Lines ◽  
Gene Therapy Vectors ◽  
Producer Cell ◽  
Novel Approaches

scholarly journals 423. The Combination of p53 Dendritic Cell Vaccine and Ad-p53 Gene Therapy Against p53 Overexpressing and p53 Deleted Tumor Cell Lines

2015 ◽  
Vol 23 ◽  
pp. S167
Author(s):  
Hiroki Saito ◽  
Koichi Kitagawa ◽  
Risa Yamasaki ◽  
Nami Katai ◽  
Naoya Morishita ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Dendritic Cell ◽  
Tumor Cell ◽  
Cell Lines ◽  
P53 Gene ◽  
Dendritic Cell Vaccine ◽  
Cell Vaccine ◽  
Tumor Cell Lines
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