Nup88 Expression in Normal Mucosa, Adenoma, Primary Adenocarcinoma and Lymph Node Metastasis in the Colorectum

Tumor Biology ◽  
2007 ◽  
Vol 28 (2) ◽  
pp. 93-99 ◽  
Author(s):  
Zhi-Yong Zhang ◽  
Zeng-Ren Zhao ◽  
Li Jiang ◽  
Jia-Chen Li ◽  
Yan-Min Gao ◽  
...  
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Normal Mucosa ◽  
Primary Adenocarcinoma ◽  
Node Metastasis

Proteomic analysis shows down-regulations of cytoplasmic carbonic anhydrases, CAI and CAII, are early events of colorectal carcinogenesis but are not correlated with lymph node metastasis

2012 ◽  
Vol 98 (6) ◽  
pp. 783-791 ◽  
Author(s):  
Ning Wang ◽  
Yang Chen ◽  
Yuchen Han ◽  
Yue Zhao ◽  
Yu Liu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Cancer Progression ◽  
Normal Mucosa ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Node Metastasis ◽  
Flight Mass Spectrometry ◽  
Differential Gel Electrophoresis

Aim The aim of the study was to screen the markedly down-regulated proteins in colorectal cancer and analyze their relationship to carcinogenesis, cancer progression and pathological aspects. Methods Proteomic analysis was preformed on six fresh colorectal cancer tissues and paired normal colorectal mucosa by two-dimensional differential gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Two markedly down-regulated proteins among the proteins, of which the expressions were significantly decreased in colorectal cancer compared to normal mucosa, were confirmed by Western Blot in 12 colorectal cancers. Their relationship to carcinogenesis, cancer progression and pathological aspects of colorectal cancer were analyzed in 64 colorectal cancer and paired normal mucosa, 27 benign polyps, and 20 lymph node metastases by immunohistochemistry. Results Two-dimensional differential gel electrophoresis analysis showed there were 2 protein spots, of which the average abundances decreased 3.62 and 3.76 fold in colorectal cancer compared to normal mucosa, respectively. They were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry as carbonic anhydrase I and II (CAI and CAII). Validation by Western Blot in 12 colorectal cancers showed there were significantly different expressions of CAI and CAII between colorectal cancer and normal mucosa (P = 0.002 and 0.027, respectively). Immunohistochemistry analysis indicated the expression of CAI and CAII was decreased from normal mucosa to benign polyps, and to colorectal cancer stepwise significantly (P <0.05). However, there were no differences in their expressions between lymph node metastasis and colorectal cancer (P >0.05). There were decreasing trends of CAI and CAII expressions from well to poor differentiation and from stage I or II to stage III or IV, but they were not statistically significant (P >0.05). Conclusions CAI and CAII are necessary enzymes of the colorectum for their normal function. Down-regulations of CAI and CAII are early events of colorectum carcinogenesis. They have no correlations with lymph node metastasis.

scholarly journals Significance of Glutathione Peroxidase 1 and Caudal-Related Homeodomain Transcription Factor in Human Gastric Adenocarcinoma

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Jing Jing Han ◽  
De Rong Xie ◽  
Li Li Wang ◽  
Ye Qing Liu ◽  
Gong Fa Wu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Glutathione Peroxidase ◽  
Normal Mucosa ◽  
Clinicopathological Features ◽  
Glutathione Peroxidase 1 ◽  
Node Metastasis ◽  
Adjacent Normal Mucosa ◽  
Extensive Lymph Node

Aim. To investigate the expressions of glutathione peroxidase 1 (GPX1) and caudal-related homeodomain transcription factor (CDX2) in GAC and their correlation with clinicopathological features and tumor cell proliferation.Methods. The expressions of GPX1, CDX2, and Ki67 were immunohistochemically evaluated in 172 GAC specimens. The association of GPX1 and CDX2 with patient’s clinicopathological features and Ki67 positive rate was analyzed statistically.Results. In 172 cases of GAC, the expression of GPX1 was weaker than that in adjacent normal mucosa, and the expression of CDX2 was higher than that in adjacent normal mucosa. High expression GPX1 strong-expression was associated with differentiation, Lauren type, WHO type and extensive lymph node metastasis of GAC. High expression of CDX2 was associated with differentiation, Lauren type, WHO type, extensive lymph node metastasis, and TNM of GAC. Survival curves showed that expressions of GPX1 and CDX2 were factors of good outcome ( and .02, resp.). According to multivariate analysis, only lymph node metastasis, TNM stage, and CDX2 expression were independently associated with survival. In addition, a strong association of GPX1 expression was noted with Ki67 and CDX2.Conclusions. The expression of GPX1 and CDX2 may play a role in the carcinogenesis, differentiation, and progression of GAC, and CDX2 may be an independent prognostic factor.

scholarly journals Prognostic Value of CD10 among Tunisian Patients with Nasopharyngeal Carcinoma

2021 ◽  
Vol 8 (11) ◽  
Author(s):  
Mokni Baizig N ◽  
◽  
Mhamdi H ◽  
El Amine El Hadj O ◽  
Goucha A ◽  
...  
Keyword(s):  
Lymph Node ◽  
Nasopharyngeal Carcinoma ◽  
Lymph Node Metastasis ◽  
Prognostic Value ◽  
Strong Association ◽  
Normal Mucosa ◽  
Control Group ◽  
Node Metastasis ◽  
Cd10 Expression ◽  
Cox Proportional Hazard Regression

Introduction: CD10 expression was identified as a marker of poor prognosis in several types of cancer. However, its impact on the survival of Tunisian NPC patients has not been discussed. So, our objective was to confirm the prognostic value of this marker, in addition to its relationship to clinicopathologic parameters. Materials and Methods: Imunohistochemistry method was performed on formalin-fixed paraffin-embedded sections of 66 cases of NPC, 6 patients with inflammatory nasopharynx and 20 normal mucosa tissues. Results: CD10 expression was detected in 66.66 % of the NPC with predominant staining in stromal cells (81.81%). While, no expression of CD10 was noted in control group (inflammatory nasopharyngeal lesions and normal nasopharynx mucosa). CD10 positive cases were correlated with increasing tumor size (p=0.001) and lymph node metastasis (p=0.034). Furthermore, with the Kaplan-Meier test, a strong association was observed between the expression of CD10 and the recurrence status (p = 0.003). Multivariate Cox proportional hazard regression analysis showed that CD10 and lymph node metastasis factors were independent predictors of time to recurrence among NPC patients with respectively p=0.001 and p=0.044. Conclusion: The present study confirms the prognostic value of CD10 expression and suggests its strong effect on aggressive behavior of nasopharyngeal carcinoma.

Upregulated CNTN1 is associated with lymph node metastasis and poor prognosis of colorectal cancer

2020 ◽  
pp. 1-9
Author(s):  
Guangyao Li ◽  
Zhengjun Zhang ◽  
Guochao Ge ◽  
Ke Fang ◽  
Jianyu Zhu
Keyword(s):  
Colorectal Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Tumor Development ◽  
Normal Mucosa ◽  
Clinical Analysis ◽  
Immunoglobulin Superfamily ◽  
Node Metastasis ◽  
Adjacent Normal Mucosa ◽  
Potential Biomarker

BACKGROUND: Contactin1 (CNTN1), a member of the immunoglobulin superfamily, is known to correlate with tumor development and progression. Although recent studies have found that elevated CNTN1 has been demonstrated in some types of cancers, the expression and prognosis of CNTN1 in colorectal cancer (CRC) are unclear. Here, we aimed to determine the clinicopathological characteristics and prognostic role of CNTN1 in CRC patients. METHODS: The protein expression of CNTN1 in tumor tissues was evaluated by immunohistochemistry. In addition, the mRNA and protein expressions of CNTN1 were examined by qRT-PCR and Western blotting analysis in 40 matched adjacent normal mucosa samples. The relationships of CNTN1 with clinicopathological data and prognosis significance were analyzed. RESULTS: Immunohistochemical consequence suggested that the protein level of CNTN1 was obviously raised in CRC compared with adjacent normal mucosa tissues (56.9% vs 10.3%, P< 0.05). In addition, we detected a significant increase in CNTN1 mRNA and protein levels in CRC tissues compared with the matched adjacent normal mucosa tissues. Moreover, increased CNTN1 exprssion was significantly associated with tumor size, lymph node metastasis (LNM), tumor node-metastasis (TNM) stage and carcino-embryonic antigen (CEA) in clinical analysis. Kaplan-Meier analysis suggested that patients with CNTN1 over-expression showed worse overall survival (OS) (P= 0.001). Multivariate analysis indicated that high CNTN1 expression was an independent predictor for poor OS in CRC patients (P= 0.028). Further analysis revealed that patients with high CNTN1 combined with LNM present accurately predicted poorer outcome. CONCLUSION: Taken together, the findingsindicate that CNTN1 plays a significant role and serve as a potential biomarker for the prediction of adverse prognosis in CRC. Intriguingly, high express of CNTN1 + LNM-present combination may improve the accuracy of prognosis.

Lymph Node Metastasis in the Biliary Tract Carcinoma: CT Evaluation

1994 ◽  
Vol 31 (1) ◽  
pp. 119
Author(s):  
Young Soo Do ◽  
Byung Hee Lee ◽  
Kie Hwan Kim ◽  
Soo Yil Chin ◽  
Ah Ra Lee
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Biliary Tract ◽  
Biliary Tract Carcinoma ◽  
Node Metastasis ◽  
Ct Evaluation
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