Engineering Transport Protein Function: Theoretical and Technical Considerations Using the Sugar-Transporting Phosphotransferase System of Escherichia coli as a Model System

2006 ◽  
Vol 11 (6) ◽  
pp. 302-307 ◽  
Author(s):  
Xavier Soberón ◽  
Milton H. Saier Jr.
Keyword(s):  
Escherichia Coli ◽  
Protein Function ◽  
Model System ◽  
Transport Protein ◽  
Phosphotransferase System ◽  
Technical Considerations

scholarly journals Biochemical Genetics of the Cryptic Gene System for Cellobiose Utilization in Escherichia coli K12

Genetics ◽  
1987 ◽  
Vol 115 (3) ◽  
pp. 419-429
Author(s):  
Maja Kricker ◽  
Barry G Hall
Keyword(s):  
Escherichia Coli ◽  
Microbial Evolution ◽  
Transport Protein ◽  
Wild Type ◽  
Phosphotransferase System ◽  
E Coli ◽  
Escherichia Coli K12 ◽  
Biochemical Assays ◽  
In The Wild

ABSTRACT The cellobiose catabolic system of Escherichia coli K12 is being used to study the role of cryptic genes in microbial evolution. Wild-type E. coli K12 do not utilize the β-glucoside sugars, arbutin, salicin and cellobiose. A Cel+ (cellobiose utilizing) mutant which grows on cellobiose, arbutin, and salicin was isolated previously from wild-type E. coli K12. Biochemical assays indicate that a cel structural gene (celT) specifies a single transport protein that is a β-glucoside specific enzyme of the phosphoenolpyruvate-dependent phosphotransferase system. The transport protein phosphorylates β-glucosides at the expense of phosphoenolpyruvate. A single phosphoglucosidase, specified by celH, hydrolyzes phosphorylated cellobiose, arbutin, and salicin. The genes of the cel system are expressed constitutively in the Cel+ mutant, whereas they are not expressed at a detectable level in the wild-type strain. The transport and hydrolase genes are simultaneously silenced or simultaneously expressed and thus constitute an operon. Cel+ strains which fail to utilize one or more β-glucosides express the transport system at a lower level than do Cel+ strains which grow on all three β-glucosides. Other strains inducibly express a gene which specifies transport of arbutin but not the other β-glucosides. The arbutin transport gene, arbT, maps outside of the cel locus.

scholarly journals Coexistence of the genes for putrescine transport protein and ornithine decarboxylase at 16 min on Escherichia coli chromosome.

1991 ◽  
Vol 266 (31) ◽  
pp. 20922-20927 ◽  
Author(s):  
K. Kashiwagi ◽  
T. Suzuki ◽  
F. Suzuki ◽  
T. Furuchi ◽  
H. Kobayashi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ornithine Decarboxylase ◽  
Transport Protein

scholarly journals 31phospho-NMR demonstration of phosphocysteine as a catalytic intermediate on the Escherichia coli phosphotransferase system EIIMtl.

1991 ◽  
Vol 266 (11) ◽  
pp. 6690-6692
Author(s):  
H H Pas ◽  
G H Meyer ◽  
W H Kruizinga ◽  
K S Tamminga ◽  
R P van Weeghel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Phosphotransferase System
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