Reference Models for Mitral Valve Tissue Engineering Based on Valve Cell Phenotype and Extracellular Matrix Analysis

2006 ◽  
Vol 183 (1) ◽  
pp. 12-23 ◽  
Author(s):  
T.C. Flanagan ◽  
A. Black ◽  
M. O’Brien ◽  
T.J. Smith ◽  
A.S. Pandit
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Mitral Valve ◽  
Matrix Analysis ◽  
Cell Phenotype ◽  
Reference Models ◽  
Valve Tissue

The Cellular and Extracellular Matrix Structure of Human Pericardium for Heart Valve Tissue Engineering

2012 ◽  
Vol 2012 (4) ◽  
pp. 38 ◽  
Author(s):  
David Schornik ◽  
Frantisek Straka ◽  
Jaroslav Masin Jaroslav Masin ◽  
Elena F Filova ◽  
Zuzana Burdikova ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Heart Valve ◽  
Matrix Structure ◽  
Valve Tissue ◽  
Heart Valve Tissue ◽  
Heart Valve Tissue Engineering ◽  
Human Pericardium

Umbilical cord as human cell source for mitral valve tissue engineering – venous vs. arterial cells

2017 ◽  
Vol 62 (5) ◽  
Author(s):  
Axel Malischewski ◽  
Ricardo Moreira ◽  
Luis Hurtado ◽  
Valentine Gesché ◽  
Thomas Schmitz-Rode ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mitral Valve ◽  
Human Cell ◽  
Native Valve ◽  
Valve Tissue ◽  
Mitral Position ◽  
Cell Source ◽  
Tissue Engineered Heart Valve ◽  
Main Components ◽  
Developed Nations

AbstractAround 2% of the population in developed nations are affected by mitral valve disease and available valvular replacements are not designed for the atrioventricular position. Recently our group developed the first tissue-engineered heart valve (TEHV) specifically designed for the mitral position – the TexMi valve. The valve recapitulates the main components of the native valve, i.e. annulus, asymmetric leaflets and the crucial

scholarly journals Extracellular Matrix Analysis of Human Renal Arteries in Both Quiescent and Active Vascular State

2020 ◽  
Vol 21 (11) ◽  
pp. 3905 ◽  
Author(s):  
Christian G.M. van Dijk ◽  
Laura Louzao-Martinez ◽  
Elise van Mulligen ◽  
Bart Boermans ◽  
Jeroen A.A. Demmers ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Renal Artery ◽  
Vascular Tissue ◽  
Proteome Analysis ◽  
Tissue Growth ◽  
Renal Arteries ◽  
Vascular Tissue Engineering ◽  
Matrix Analysis ◽  
Ecm Proteins

In vascular tissue engineering strategies, the addition of vascular-specific extracellular matrix (ECM) components may better mimic the in vivo microenvironment and potentially enhance cell–matrix interactions and subsequent tissue growth. For this purpose, the exact composition of the human vascular ECM first needs to be fully characterized. Most research has focused on characterizing ECM components in mature vascular tissue; however, the developing fetal ECM matches the active environment required in vascular tissue engineering more closely. Consequently, we characterized the ECM protein composition of active (fetal) and quiescent (mature) renal arteries using a proteome analysis of decellularized tissue. The obtained human fetal renal artery ECM proteome dataset contains higher levels of 15 ECM proteins versus the mature renal artery ECM proteome, whereas 16 ECM proteins showed higher levels in the mature tissue compared to fetal. Elastic ECM proteins EMILIN1 and FBN1 are significantly enriched in fetal renal arteries and are mainly produced by cells of mesenchymal origin. We functionally tested the role of EMILIN1 and FBN1 by anchoring the ECM secreted by vascular smooth muscle cells (SMCs) to glass coverslips. This ECM layer was depleted from either EMILIN1 or FBN1 by using siRNA targeting of the SMCs. Cultured endothelial cells (ECs) on this modified ECM layer showed alterations on the transcriptome level of multiple pathways, especially the Rho GTPase controlled pathways. However, no significant alterations in adhesion, migration or proliferation were observed when ECs were cultured on EMILIN1- or FNB1-deficient ECM. To conclude, the proteome analysis identified unique ECM proteins involved in the embryonic development of renal arteries. Alterations in transcriptome levels of ECs cultured on EMILIN1- or FBN1-deficient ECM showed that these candidate proteins could affect the endothelial (regenerative) response.

scholarly journals Stabilized Collagen and Elastin-Based Scaffolds for Mitral Valve Tissue Engineering

2016 ◽  
Vol 22 (21-22) ◽  
pp. 1241-1251 ◽  
Author(s):  
Christopher Deborde ◽  
Dan Teodor Simionescu ◽  
Cristopher Wright ◽  
Jun Liao ◽  
Leslie Neil Sierad ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mitral Valve ◽  
Valve Tissue

Extracellular matrix production by adipose-derived stem cells: Implications for heart valve tissue engineering

Biomaterials ◽  
2011 ◽  
Vol 32 (1) ◽  
pp. 119-127 ◽  
Author(s):  
Francesca Colazzo ◽  
Padmini Sarathchandra ◽  
Ryszard T. Smolenski ◽  
Adrian H. Chester ◽  
Yuan-Tsan Tseng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Extracellular Matrix ◽  
Heart Valve ◽  
Adipose Derived Stem Cells ◽  
Valve Tissue ◽  
Extracellular Matrix Production ◽  
Heart Valve Tissue ◽  
Matrix Production ◽  
Heart Valve Tissue Engineering
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