Allergic and Anaphylactic Response to Sesame Seeds in Mice: Identification of Ses i 3 and Basic Subunit of 11s Globulins as Allergens

2006 ◽  
Vol 140 (3) ◽  
pp. 270-276 ◽  
Author(s):  
Lalitha Navuluri ◽  
Sitaram Parvataneni ◽  
Hanem Hassan ◽  
Neil P. Birmingham ◽  
Caleb Kelly ◽  
...  
Keyword(s):  
Sesame Seeds ◽  
Basic Subunit ◽  
Anaphylactic Response ◽  
11S Globulins

Crystalline order to a resolution of 4.7 A in the sheath of Methanospirilium hungatii: A cross-beta structure

1984 ◽  
Vol 42 ◽  
pp. 214-215
Author(s):  
Murray Stewart ◽  
T.J. Beveridge ◽  
D. Sprott
Keyword(s):  
Cell Wall ◽  
Single Layer ◽  
Uranyl Acetate ◽  
Optical Diffraction ◽  
Tube Axis ◽  
Basic Subunit ◽  
Beta Structure ◽  
Diffraction Patterns ◽  
Protein Treatment ◽  
General Appearance

The archaebacterium Methanospirillum hungatii has a sheath as part of its cell wall which is composed mainly of protein. Treatment with dithiothreitol or NaOH released the intact sheaths and electron micrographs of this material negatively stained with uranyl acetate showed flattened hollow tubes, about 0.5 μm diameter and several microns long, in which the patterns from the top and bottom were superimposed. Single layers, derived from broken tubes, were also seen and were more simply analysed. Figure 1 shows the general appearance of a single layer. There was a faint axial periodicity at 28.5 A, which was stronger at irregular multiples of 28.5 A (3 and 4 times were most common), and fine striations were also seen at about 3° to the tube axis. Low angle electron diffraction patterns (not shown) and optical diffraction patterns (Fig. 2) from these layers showed a complex meridian (as a result of the irregular nature of the repeat along the tube axis) which showed a clear maximum at 28.5 A, consistent with the basic subunit spacing.

Cryomicroscopy of crotoxin complex crystals at 400 KV

1989 ◽  
Vol 47 ◽  
pp. 826-827
Author(s):  
Jaap Brink ◽  
Wah Chiu
Keyword(s):  
Signal To Noise Ratio ◽  
3D Structure ◽  
Three Dimensional ◽  
Carbon Films ◽  
Depth Of Field ◽  
Basic Subunit ◽  
Ewald Sphere ◽  
Diffraction Patterns ◽  
Complex Crystals ◽  
Acidic Subunit

The crotoxin complex is a potent neurotoxin composed of a basic subunit (Mr = 12,000) and an acidic subunit (M = 10,000). The basic subunit possesses phospholipase activity whereas the acidic subunit shows no enzymatic activity at all. The complex's toxocity is expressed both pre- and post-synaptically. The crotoxin complex forms thin crystals suitable for electron crystallography. The crystals diffract up to 0.16 nm in the microscope, whereas images show reflections out to 0.39 nm2. Ultimate goal in this study is to obtain a three-dimensional (3D-) structure map of the protein around 0.3 nm resolution. Use of 100 keV electrons in this is limited; the unit cell's height c of 25.6 nm causes problems associated with multiple scattering, radiation damage, limited depth of field and a more pronounced Ewald sphere curvature. In general, they lead to projections of the unit cell, which at the desired resolution, cannot be interpreted following the weak-phase approximation. Circumventing this problem is possible through the use of 400 keV electrons. Although the overall contrast is lowered due to a smaller scattering cross-section, the signal-to-noise ratio of especially higher order reflections will improve due to a smaller contribution of inelastic scattering. We report here our preliminary results demonstrating the feasability of the data collection procedure at 400 kV.Crystals of crotoxin complex were prepared on carbon-covered holey-carbon films, quench frozen in liquid ethane, inserted into a Gatan 626 holder, transferred into a JEOL 4000EX electron microscope equipped with a pair of anticontaminators operating at −184°C and examined under low-dose conditions. Selected area electron diffraction patterns (EDP's) and images of the crystals were recorded at 400 kV and −167°C with dose levels of 5 and 9.5 electrons/Å, respectively.

scholarly journals Lignans of Sesame (Sesamum indicum L.): A Comprehensive Review

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 883
Author(s):  
Mebeaselassie Andargie ◽  
Maria Vinas ◽  
Anna Rathgeb ◽  
Evelyn Möller ◽  
Petr Karlovsky
Keyword(s):  
Biological Activities ◽  
Chemical Properties ◽  
Transformation Products ◽  
Extensive Literature ◽  
Controversial Issues ◽  
Sesame Seeds ◽  
Historical Texts ◽  
Health Promoting ◽  
Processed Products

Major lignans of sesame sesamin and sesamolin are benzodioxol--substituted furofurans. Sesamol, sesaminol, its epimers, and episesamin are transformation products found in processed products. Synthetic routes to all lignans are known but only sesamol is synthesized industrially. Biosynthesis of furofuran lignans begins with the dimerization of coniferyl alcohol, followed by the formation of dioxoles, oxidation, and glycosylation. Most genes of the lignan pathway in sesame have been identified but the inheritance of lignan content is poorly understood. Health-promoting properties make lignans attractive components of functional food. Lignans enhance the efficiency of insecticides and possess antifeedant activity, but their biological function in plants remains hypothetical. In this work, extensive literature including historical texts is reviewed, controversial issues are critically examined, and errors perpetuated in literature are corrected. The following aspects are covered: chemical properties and transformations of lignans; analysis, purification, and total synthesis; occurrence in Seseamum indicum and related plants; biosynthesis and genetics; biological activities; health-promoting properties; and biological functions. Finally, the improvement of lignan content in sesame seeds by breeding and biotechnology and the potential of hairy roots for manufacturing lignans in vitro are outlined.

Anaphylactic response to blue dye during sentinel lymph node biopsy

2011 ◽  
Vol 20 (1) ◽  
pp. e55-e59 ◽  
Author(s):  
Corinne Bézu ◽  
Charles Coutant ◽  
Anne Salengro ◽  
Emile Daraï ◽  
Roman Rouzier ◽  
...  
Keyword(s):  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Sentinel Lymph Node Biopsy ◽  
Lymph Node Biopsy ◽  
Blue Dye ◽  
Node Biopsy ◽  
Anaphylactic Response

Cephalosporins Can Be Given to Penicillin-allergic Patients Who Do Not Exhibit an Anaphylactic Response

2001 ◽  
Vol 13 (8) ◽  
pp. 561-564 ◽  
Author(s):  
Evan J Goodman ◽  
Michelle J Morgan ◽  
Pat A Johnson ◽  
Brenda A Nichols ◽  
Nevia Denk ◽  
...  
Keyword(s):  
Anaphylactic Response

Active Bronchial Anaphylaxis in the Rat

1974 ◽  
Vol 52 (6) ◽  
pp. 1114-1118 ◽  
Author(s):  
L. M. Stotland ◽  
N. N. Share
Keyword(s):  
Allergic Asthma ◽  
Specific Antigen ◽  
Female Rats ◽  
Sprague Dawley ◽  
Egg Albumin ◽  
Tracheal Pressure ◽  
Resultant Increase ◽  
Dose Responses ◽  
Reaginic Antibodies ◽  
Anaphylactic Response

Sprague–Dawley female rats were immunized with egg albumin (EA) in aluminum hydroxide gel (AHG) and with or without Bacillus pertussis vaccine (BPV). Fourteen days later, the animals were anesthetized and challenged with EA intravenously. The resultant increase in tracheal pressure was recorded as an index of anaphylactic bronchoconstriction. Ventilation with tracheal pressures of 6 cm H2O (588 N/m2) allowed maximal development of bronchoconstrictor responses to specific antigen challenge that were similar in both pithed and pentobarbital-anesthetized preparations. Forced reinflation of the lungs did not affect the magnitude of the response but did facilitate its recovery. Serum titers evaluated by 3-h and 72-h passive cutaneous anaphylactic reactions indicated that reaginic antibodies were primarily involved, although other immunoglobulins may have played a contributory role. Antigen dose-responses were similar for both the EA-AHG and EA-AHG-BPV immunized groups of animals despite lower reaginic serum titers for the former group. Thus, an immediate-type bronchial anaphylactic response mediated primarily by reaginic antibodies can be elicited in rats and quantitatively assessed. The potential immunologic similarity of these animals to human allergic asthma suggests their utility for further investigation.

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