Metallothionein Isoform 3 Gene Is Differentially Expressed in Corticotropin-Producing Pituitary Adenomas

2005 ◽  
Vol 82 (3-4) ◽  
pp. 208-214 ◽  
Author(s):  
R.R. Giorgi ◽  
M.L.C. Correa-Giannella ◽  
A.P.M. Casarini ◽  
M.C. Machado ◽  
M.D. Bronstein ◽  
...  
Keyword(s):  
Pituitary Adenomas ◽  
Differentially Expressed ◽  
Metallothionein Isoform 3

scholarly journals USP8 Status Affects the Immune Landscape of Corticotroph Pituitary Adenomas

2021 ◽  
Author(s):  
Dahlia Greidinger ◽  
Ronit Mor-Cohen ◽  
Roni Zemet ◽  
Nitzan Maixner ◽  
Amit Tirosh
Keyword(s):  
Pituitary Adenomas ◽  
Immune Cell ◽  
Cell Types ◽  
Differentially Expressed ◽  
Toll Like Receptor ◽  
M1 Macrophages ◽  
Mutation Status ◽  
Specific Protease ◽  
Signature Recognition ◽  
Ubiquitin Specific Protease

Abstract Purpose Activating somatic mutations in ubiquitin-specific protease-8 (USP8), encoding a deubiquitinating protein, are found in approximately 30% of corticotroph-derived pituitary adenomas (CPA). USP8 has immunomodulating properties that were demonstrated in non-tumoral diseases. Our study aims to assess the influence of USP8 mutation status on the immune tumor microenvironment (iTME) of CPAs. Methods We analyzed 20 PCAs by RNA sequencing. In six of them, USP8 mutations were detected. We assessed the immune landscape of tumors by quantifying 22 immune cell types based on the CIBERSORT transcriptome signature-recognition algorithms. Also, we performed a pathway analysis for genes that were differentially expressed between groups using the Wikipathways 2019 and Reactome 2016 databases and using the EnrichR platform results. Results CPA with activating USP8 mutations were associated with "cold" iTME compared with wild type USP8 CPA. This "cold" iTME was reflected by lower fractions of B cells, CD4, regulatory and gamma/delta T cells, natural killer cells, M0 and M1 macrophages, dendritic cells and eosinophils (p < 0.05 for all comparisons). Pathways altered by the presence of USP8 mutation, based on the most differentially expressed genes (3,061 genes) included Microglia Pathogen Phagocytosis and multiple toll-like receptor signaling pathways (p < 0.0001). Conclusion USP8 status affects the immune landscape of corticotroph pituitary adenomas, with USP8 mutation associated with "cold" iTME.

scholarly journals Identification of circular RNAs in the immature and mature rat anterior pituitary

2019 ◽  
Vol 240 (3) ◽  
pp. 393-402 ◽  
Author(s):  
Dong-Xu Han ◽  
Chang-Jiang Wang ◽  
Xu-Lei Sun ◽  
Jian-Bo Liu ◽  
Hao Jiang ◽  
...  
Keyword(s):  
Pituitary Adenomas ◽  
Anterior Pituitary ◽  
Differentially Expressed ◽  
Circular Rnas ◽  
Illumina Platform ◽  
Immature Rat ◽  
Kegg Pathways ◽  
New Class ◽  
Rat Pituitary ◽  
Mirna Sponges

Circular RNAs (circRNAs) are a new class of RNA that have a stable structure characterized by covalently closed circular molecules and are involved in invasive pituitary adenomas and recurrent clinically nonfunctioning pituitary adenomas. However, information on circRNAs in the normal pituitary, especially in rats, is limited. In this study, we identified 4123 circRNAs in the immature (D15) and mature (D120) rat anterior pituitary using the Illumina platform, and 32 differentially expressed circRNAs were found. A total of 150 Gene Ontology terms were significantly enriched, and 16 KEGG pathways were found to contain differentially expressed genes. Moreover, we randomly selected eight highly expressed circRNAs and detected their relative expression levels in the mature and immature rat pituitary by qPCR. In addition, we predicted 90 interactions between 53 circRNAs and 57 miRNAs using miRanda. Notably, circ_0000964 and circ_0001303 are potential miRNA sponges that may regulate the Fshb gene. The expression profile of circRNAs in the immature and mature rat anterior pituitary may provide more information about the roles of circRNAs in the development and reproduction in mammals.

scholarly journals Sexual Dimorphism in Cellular and Molecular Features in Human ACTH-Secreting Pituitary Adenomas

Cancers ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 669 ◽  
Author(s):  
Francesca Pecori Giraldi ◽  
Maria Francesca Cassarino ◽  
Antonella Sesta ◽  
Mariarosa Terreni ◽  
Giovanni Lasio ◽  
...  
Keyword(s):  
Sexual Dimorphism ◽  
Differentially Expressed Genes ◽  
Cushing’S Disease ◽  
Pituitary Adenomas ◽  
Differentially Expressed ◽  
Cushing's Disease ◽  
Female Patients ◽  
Molecular Features ◽  
Male Patients ◽  
Acth Secreting

(1) Background. Cushing’s disease presents gender disparities in prevalence and clinical course. Little is known, however, about sexual dimorphism at the level of the corticotrope adenoma itself. The aim of the present study was to evaluate molecular features of ACTH-secreting pituitary adenomas collected from female and male patients with Cushing’s disease. (2) Methods. We analyzed 153 ACTH-secreting adenomas collected from 31 men and 122 women. Adenomas were established in culture and ACTH synthesis and secretion assessed in basal conditions as well as during incubation with CRH or dexamethasone. Concurrently, microarray analysis was performed on formalin-fixed specimens and differences in the expression profiles between specimens from male and female patients identified. (3) Results. ACTH medium concentrations in adenomas obtained from male patients were significantly lower than those observed in adenomas from female patients. This could be observed for baseline as well as modulated secretion. Analysis of corticotrope transcriptomes revealed considerable similarities with few, selected differences in functional annotations. Differentially expressed genes comprised genes with known sexual dimorphism, genes involved in tumour development and genes relevant to pituitary pathophysiology. (4) Conclusions. Our study shows for the first time that human corticotrope adenomas present sexual dimorphism and underlines the need for a gender-dependent analysis of these tumours. Differentially expressed genes may represent the basis for gender-tailored target therapy.

scholarly journals Notch system is differentially expressed and activated in pituitary adenomas of distinct histotype, tumor cell lines and normal pituitaries

Oncotarget ◽  
2017 ◽  
Vol 8 (34) ◽  
pp. 57072-57088 ◽  
Author(s):  
Sofia Perrone ◽  
Lautaro Zubeldia-Brenner ◽  
Elias Gazza ◽  
Gianina Demarchi ◽  
Leticia Baccarini ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Pituitary Adenomas ◽  
Differentially Expressed ◽  
Tumor Cell Lines

scholarly journals LncRNA-mRNA Expression Pattern in Invasive Pituitary Adenomas: A Microarray Analysis

2021 ◽  
Author(s):  
Chao Peng ◽  
Shuaikai Wang ◽  
Jinxiu Yu ◽  
Xiaoyi Deng ◽  
Zhishan Chen ◽  
...  
Keyword(s):  
Pituitary Adenomas ◽  
Roc Analysis ◽  
Gene Prediction ◽  
Expression Patterns ◽  
Differentially Expressed ◽  
Significant Differential Expression ◽  
Expression Levels ◽  
Qrt Pcr ◽  
Go Enrichment ◽  
Diagnostic Values

Abstract Backgrounds: Long non-coding RNAs (lncRNAs) play important roles in tumorigenesis and progression of various cancer types; however, their roles in the development of invasive pituitary adenomas (PAs) remain to be investigated.Methods: lncRNA microarray was performed in three invasive and three noninvasive PAs. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed, and coexpression networks between lncRNA and mRNA were constructed. Furthermore, three differentially expressed lncRNAs were selected for validation by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) in PA samples. The diagnostic values of these three lncRNAs were further evaluated by receiver operating characteristic (ROC) analysis.Results: A total of 8872 lncRNAs were identified in invasive and paired noninvasive PAs using lncRNA microarray. Among these, the differentially expressed lncRNAs included 81 that were upregulated and 165 that were downregulated. GO enrichment and KEGG pathway analysis showed that these differentially expressed lncRNAs were associated with post-translational modifications of proteins. Furthermore, we performed target gene prediction and coexpression analysis. The interrelationships between the lncRNAs and mRNAs with significant differential expression were identified. Additionally, three differentially expressed lncRNAs were selected for validation in 41 PA samples by qRT-PCR. The expression levels of FAM182B, LOC105371531, and LOC105375785 in the invasive PAs were significantly (P < 0.05) lower than in the noninvasive PAs, and these results were consistent with the microarray data. ROC analysis suggested that FAM182B and LOC105375785 expression levels could be used to distinguish invasive PAs from noninvasive PAs.Conclusion: Our findings demonstrated the lncRNAs expression patterns in invasive PAs. Thus, FAM182B and LOC105375785 may be involved in the invasiveness of PAs and serve as new candidate biomarkers for the diagnosis of invasive PAs.

scholarly journals Identification of Differentially Expressed Genes in Pituitary Adenomas by Integrating Analysis of Microarray Data

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Peng Zhao ◽  
Wei Hu ◽  
Hongyun Wang ◽  
Shengyuan Yu ◽  
Chuzhong Li ◽  
...  
Keyword(s):  
Pituitary Adenoma ◽  
Differentially Expressed Genes ◽  
Microarray Data ◽  
Gene Function ◽  
Pituitary Adenomas ◽  
Function Analysis ◽  
Differentially Expressed ◽  
Integrated Analysis ◽  
Ppi Networks ◽  
Gene Function Analysis

Pituitary adenomas, monoclonal in origin, are the most common intracranial neoplasms. Altered gene expression as well as somatic mutations is detected frequently in pituitary adenomas. The purpose of this study was to detect differentially expressed genes (DEGs) and biological processes during tumor formation of pituitary adenomas. We performed an integrated analysis of publicly available GEO datasets of pituitary adenomas to identify DEGs between pituitary adenomas and normal control (NC) tissues. Gene function analysis including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and protein-protein interaction (PPI) networks analysis was conducted to interpret the biological role of those DEGs. In this study we detected 3994 DEGs (2043 upregulated and 1951 downregulated) in pituitary adenoma through an integrated analysis of 5 different microarray datasets. Gene function analysis revealed that the functions of those DEGs were highly correlated with the development of pituitary adenoma. This integrated analysis of microarray data identified some genes and pathways associated with pituitary adenoma, which may help to understand the pathology underlying pituitary adenoma and contribute to the successful identification of therapeutic targets for pituitary adenoma.

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